Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14380 and AMB_RS14385 are separated by 186 nucleotides AMB_RS14385 and AMB_RS14390 are separated by 1 nucleotides AMB_RS14390 and AMB_RS14395 are separated by 82 nucleotides AMB_RS14395 and AMB_RS14400 are separated by 11 nucleotides AMB_RS14400 and AMB_RS14405 are separated by 134 nucleotides
AMB_RS14380: AMB_RS14380 - hypothetical protein, at 3,059,684 to 3,060,004
_RS14380
AMB_RS14385: AMB_RS14385 - thiamine phosphate synthase, at 3,060,191 to 3,060,829
_RS14385
AMB_RS14390: AMB_RS14390 - TIGR00730 family Rossman fold protein, at 3,060,831 to 3,061,412
_RS14390
AMB_RS14395: AMB_RS14395 - tRNA-Val, at 3,061,495 to 3,061,569
_RS14395
AMB_RS14400: AMB_RS14400 - hypothetical protein, at 3,061,581 to 3,062,192
_RS14400
AMB_RS14405: AMB_RS14405 - type 1 glutamine amidotransferase, at 3,062,327 to 3,062,917
_RS14405
Position (kb)
3060
3061
3062 Strain fitness (log2 ratio)
-2
-1
0
1 at 3060.063 kb on + strand at 3060.063 kb on + strand at 3060.063 kb on + strand at 3060.064 kb on - strand at 3060.064 kb on - strand at 3060.064 kb on - strand at 3060.064 kb on - strand at 3060.064 kb on - strand at 3060.089 kb on + strand at 3060.089 kb on + strand at 3060.090 kb on - strand at 3060.883 kb on - strand at 3060.883 kb on - strand at 3060.942 kb on + strand, within AMB_RS14390 at 3061.365 kb on + strand at 3061.366 kb on - strand at 3061.671 kb on + strand, within AMB_RS14400 at 3061.671 kb on + strand, within AMB_RS14400 at 3061.671 kb on + strand, within AMB_RS14400 at 3061.672 kb on - strand, within AMB_RS14400 at 3061.672 kb on - strand, within AMB_RS14400 at 3061.672 kb on - strand, within AMB_RS14400 at 3061.793 kb on - strand, within AMB_RS14400 at 3061.924 kb on + strand, within AMB_RS14400 at 3061.925 kb on - strand, within AMB_RS14400 at 3061.958 kb on - strand, within AMB_RS14400 at 3061.984 kb on + strand, within AMB_RS14400 at 3062.183 kb on + strand at 3062.184 kb on - strand at 3062.184 kb on - strand at 3062.226 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 3,060,063 + -1.4 3,060,063 + -2.7 3,060,063 + +0.9 3,060,064 - -1.1 3,060,064 - +0.0 3,060,064 - +0.5 3,060,064 - -0.2 3,060,064 - -1.1 3,060,089 + +0.1 3,060,089 + -0.1 3,060,090 - +0.1 3,060,883 - +1.2 3,060,883 - +0.1 3,060,942 + AMB_RS14390 0.19 -0.1 3,061,365 + +0.2 3,061,366 - +0.4 3,061,671 + AMB_RS14400 0.15 +0.2 3,061,671 + AMB_RS14400 0.15 -0.6 3,061,671 + AMB_RS14400 0.15 -1.4 3,061,672 - AMB_RS14400 0.15 +0.5 3,061,672 - AMB_RS14400 0.15 -2.7 3,061,672 - AMB_RS14400 0.15 +0.3 3,061,793 - AMB_RS14400 0.35 -1.4 3,061,924 + AMB_RS14400 0.56 -1.7 3,061,925 - AMB_RS14400 0.56 -0.3 3,061,958 - AMB_RS14400 0.62 -1.4 3,061,984 + AMB_RS14400 0.66 +0.8 3,062,183 + -0.9 3,062,184 - +0.1 3,062,184 - +1.0 3,062,226 + -0.6
Or see this region's nucleotide sequence