Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS13575 and AMB_RS13580 are separated by 158 nucleotides AMB_RS13580 and AMB_RS13585 overlap by 9 nucleotides AMB_RS13585 and AMB_RS13590 are separated by 108 nucleotides
AMB_RS13575: AMB_RS13575 - hypothetical protein, at 2,892,026 to 2,892,799
_RS13575
AMB_RS13580: AMB_RS13580 - ribonuclease I, at 2,892,958 to 2,893,602
_RS13580
AMB_RS13585: AMB_RS13585 - alpha/beta hydrolase, at 2,893,594 to 2,894,289
_RS13585
AMB_RS13590: AMB_RS13590 - thiazole synthase, at 2,894,398 to 2,895,372
_RS13590
Position (kb)
2893
2894
2895 Strain fitness (log2 ratio)
-2
-1
0
1 at 2892.625 kb on - strand, within AMB_RS13575 at 2892.637 kb on - strand, within AMB_RS13575 at 2892.813 kb on - strand at 2892.813 kb on - strand at 2892.834 kb on - strand at 2892.847 kb on + strand at 2892.855 kb on + strand at 2892.855 kb on + strand at 2892.855 kb on + strand at 2892.855 kb on + strand at 2892.856 kb on - strand at 2893.069 kb on + strand, within AMB_RS13580 at 2893.069 kb on + strand, within AMB_RS13580 at 2893.102 kb on + strand, within AMB_RS13580 at 2893.102 kb on + strand, within AMB_RS13580 at 2893.160 kb on - strand, within AMB_RS13580 at 2893.160 kb on - strand, within AMB_RS13580 at 2893.160 kb on - strand, within AMB_RS13580 at 2893.192 kb on + strand, within AMB_RS13580 at 2893.193 kb on - strand, within AMB_RS13580 at 2893.193 kb on - strand, within AMB_RS13580 at 2893.193 kb on - strand, within AMB_RS13580 at 2893.348 kb on + strand, within AMB_RS13580 at 2893.349 kb on - strand, within AMB_RS13580 at 2893.512 kb on + strand, within AMB_RS13580 at 2893.512 kb on + strand, within AMB_RS13580 at 2893.512 kb on + strand, within AMB_RS13580 at 2893.512 kb on + strand, within AMB_RS13580 at 2893.513 kb on - strand, within AMB_RS13580 at 2893.513 kb on - strand, within AMB_RS13580 at 2893.595 kb on + strand at 2893.595 kb on + strand at 2893.596 kb on - strand at 2893.759 kb on + strand, within AMB_RS13585 at 2894.090 kb on + strand, within AMB_RS13585 at 2894.090 kb on + strand, within AMB_RS13585 at 2894.090 kb on + strand, within AMB_RS13585 at 2894.090 kb on + strand, within AMB_RS13585 at 2894.090 kb on + strand, within AMB_RS13585 at 2894.090 kb on + strand, within AMB_RS13585 at 2894.091 kb on - strand, within AMB_RS13585 at 2894.091 kb on - strand, within AMB_RS13585 at 2894.091 kb on - strand, within AMB_RS13585 at 2894.091 kb on - strand, within AMB_RS13585 at 2894.348 kb on - strand at 2894.378 kb on + strand at 2894.379 kb on - strand at 2894.379 kb on - strand at 2894.379 kb on - strand at 2894.379 kb on - strand at 2894.379 kb on - strand at 2894.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 2,892,625 - AMB_RS13575 0.77 -0.1 2,892,637 - AMB_RS13575 0.79 -0.2 2,892,813 - +0.1 2,892,813 - +0.0 2,892,834 - -0.1 2,892,847 + -0.5 2,892,855 + +1.5 2,892,855 + -0.5 2,892,855 + -1.8 2,892,855 + +0.5 2,892,856 - -1.5 2,893,069 + AMB_RS13580 0.17 +0.1 2,893,069 + AMB_RS13580 0.17 -0.1 2,893,102 + AMB_RS13580 0.22 -1.3 2,893,102 + AMB_RS13580 0.22 -1.7 2,893,160 - AMB_RS13580 0.31 +0.1 2,893,160 - AMB_RS13580 0.31 -0.9 2,893,160 - AMB_RS13580 0.31 -0.4 2,893,192 + AMB_RS13580 0.36 +1.5 2,893,193 - AMB_RS13580 0.36 +0.2 2,893,193 - AMB_RS13580 0.36 +0.1 2,893,193 - AMB_RS13580 0.36 -1.1 2,893,348 + AMB_RS13580 0.60 +1.5 2,893,349 - AMB_RS13580 0.61 -1.3 2,893,512 + AMB_RS13580 0.86 +0.4 2,893,512 + AMB_RS13580 0.86 -1.1 2,893,512 + AMB_RS13580 0.86 +0.5 2,893,512 + AMB_RS13580 0.86 +0.1 2,893,513 - AMB_RS13580 0.86 +0.3 2,893,513 - AMB_RS13580 0.86 -0.3 2,893,595 + -0.2 2,893,595 + -1.7 2,893,596 - +0.5 2,893,759 + AMB_RS13585 0.24 -1.7 2,894,090 + AMB_RS13585 0.71 -0.5 2,894,090 + AMB_RS13585 0.71 -1.2 2,894,090 + AMB_RS13585 0.71 -0.5 2,894,090 + AMB_RS13585 0.71 +0.3 2,894,090 + AMB_RS13585 0.71 -1.5 2,894,090 + AMB_RS13585 0.71 -0.5 2,894,091 - AMB_RS13585 0.71 -2.3 2,894,091 - AMB_RS13585 0.71 -1.7 2,894,091 - AMB_RS13585 0.71 -1.7 2,894,091 - AMB_RS13585 0.71 -0.9 2,894,348 - -2.6 2,894,378 + +0.0 2,894,379 - +0.6 2,894,379 - -1.7 2,894,379 - -1.9 2,894,379 - -1.7 2,894,379 - -0.9 2,894,379 - +0.9
Or see this region's nucleotide sequence