Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12450 and AMB_RS12455 are separated by 86 nucleotides AMB_RS12455 and AMB_RS12460 are separated by 41 nucleotides AMB_RS12460 and AMB_RS12465 are separated by 164 nucleotides AMB_RS12465 and AMB_RS12470 are separated by 201 nucleotides AMB_RS12470 and AMB_RS12475 are separated by 42 nucleotides
AMB_RS12450: AMB_RS12450 - class I SAM-dependent methyltransferase, at 2,656,956 to 2,657,981
_RS12450
AMB_RS12455: AMB_RS12455 - hypothetical protein, at 2,658,068 to 2,658,391
_RS12455
AMB_RS12460: AMB_RS12460 - ferredoxin, at 2,658,433 to 2,658,786
_RS12460
AMB_RS12465: AMB_RS12465 - DUF4170 domain-containing protein, at 2,658,951 to 2,659,358
_RS12465
AMB_RS12470: AMB_RS12470 - hypothetical protein, at 2,659,560 to 2,659,826
_RS12470
AMB_RS12475: AMB_RS12475 - FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO, at 2,659,869 to 2,661,212
_RS12475
Position (kb)
2658
2659
2660 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2657.993 kb on + strand at 2657.993 kb on + strand at 2657.993 kb on + strand at 2657.993 kb on + strand at 2657.994 kb on - strand at 2657.994 kb on - strand at 2657.994 kb on - strand at 2658.100 kb on + strand at 2658.101 kb on - strand, within AMB_RS12455 at 2658.250 kb on + strand, within AMB_RS12455 at 2658.746 kb on + strand, within AMB_RS12460 at 2658.746 kb on + strand, within AMB_RS12460 at 2658.747 kb on - strand, within AMB_RS12460 at 2658.747 kb on - strand, within AMB_RS12460 at 2658.759 kb on - strand at 2658.794 kb on - strand at 2658.836 kb on - strand at 2658.847 kb on - strand at 2658.920 kb on + strand at 2658.939 kb on + strand at 2659.403 kb on - strand at 2659.559 kb on + strand at 2659.560 kb on - strand at 2659.560 kb on - strand at 2659.560 kb on - strand at 2659.560 kb on - strand at 2659.665 kb on + strand, within AMB_RS12470 at 2659.665 kb on + strand, within AMB_RS12470 at 2659.665 kb on + strand, within AMB_RS12470 at 2659.666 kb on - strand, within AMB_RS12470 at 2659.666 kb on - strand, within AMB_RS12470 at 2659.856 kb on + strand at 2659.857 kb on - strand at 2660.269 kb on + strand, within AMB_RS12475 at 2660.270 kb on - strand, within AMB_RS12475 at 2660.270 kb on - strand, within AMB_RS12475
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 2,657,993 + -0.0 2,657,993 + -0.8 2,657,993 + -0.1 2,657,993 + -0.5 2,657,994 - -0.4 2,657,994 - -1.1 2,657,994 - +0.2 2,658,100 + -0.1 2,658,101 - AMB_RS12455 0.10 +0.1 2,658,250 + AMB_RS12455 0.56 -0.5 2,658,746 + AMB_RS12460 0.88 -2.8 2,658,746 + AMB_RS12460 0.88 +0.7 2,658,747 - AMB_RS12460 0.89 +0.2 2,658,747 - AMB_RS12460 0.89 -0.8 2,658,759 - +0.4 2,658,794 - -2.2 2,658,836 - -0.0 2,658,847 - +0.2 2,658,920 + +0.4 2,658,939 + +0.5 2,659,403 - -2.0 2,659,559 + -0.8 2,659,560 - -2.5 2,659,560 - -0.2 2,659,560 - -3.0 2,659,560 - -1.3 2,659,665 + AMB_RS12470 0.39 -1.1 2,659,665 + AMB_RS12470 0.39 -0.4 2,659,665 + AMB_RS12470 0.39 -1.7 2,659,666 - AMB_RS12470 0.40 -0.8 2,659,666 - AMB_RS12470 0.40 +0.5 2,659,856 + -1.1 2,659,857 - -2.0 2,660,269 + AMB_RS12475 0.30 -2.1 2,660,270 - AMB_RS12475 0.30 -0.1 2,660,270 - AMB_RS12475 0.30 -2.2
Or see this region's nucleotide sequence