Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11840 and AMB_RS11845 overlap by 4 nucleotides AMB_RS11845 and AMB_RS11850 are separated by 1 nucleotides AMB_RS11850 and AMB_RS11855 are separated by 12 nucleotides AMB_RS11855 and AMB_RS11860 are separated by 277 nucleotides
AMB_RS11840: AMB_RS11840 - 6,7-dimethyl-8-ribityllumazine synthase, at 2,550,399 to 2,550,851
_RS11840
AMB_RS11845: AMB_RS11845 - N utilization substance protein B, at 2,550,848 to 2,551,318
_RS11845
AMB_RS11850: AMB_RS11850 - thiamine-phosphate kinase, at 2,551,320 to 2,552,331
_RS11850
AMB_RS11855: AMB_RS11855 - hypothetical protein, at 2,552,344 to 2,552,796
_RS11855
AMB_RS11860: AMB_RS11860 - sodium-translocating pyrophosphatase, at 2,553,074 to 2,555,155
_RS11860
Position (kb)
2551
2552
2553 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2550.337 kb on + strand at 2550.338 kb on - strand at 2550.378 kb on + strand at 2550.378 kb on + strand at 2550.382 kb on + strand at 2550.382 kb on + strand at 2550.382 kb on + strand at 2550.382 kb on + strand at 2550.382 kb on + strand at 2550.382 kb on + strand at 2552.330 kb on - strand at 2552.401 kb on + strand, within AMB_RS11855 at 2552.401 kb on + strand, within AMB_RS11855 at 2552.401 kb on + strand, within AMB_RS11855 at 2552.401 kb on + strand, within AMB_RS11855 at 2552.402 kb on - strand, within AMB_RS11855 at 2552.497 kb on + strand, within AMB_RS11855 at 2552.497 kb on + strand, within AMB_RS11855 at 2552.497 kb on + strand, within AMB_RS11855 at 2552.497 kb on + strand, within AMB_RS11855 at 2552.497 kb on + strand, within AMB_RS11855 at 2552.498 kb on - strand, within AMB_RS11855 at 2552.498 kb on - strand, within AMB_RS11855 at 2552.498 kb on - strand, within AMB_RS11855 at 2552.498 kb on - strand, within AMB_RS11855 at 2552.638 kb on + strand, within AMB_RS11855 at 2552.659 kb on + strand, within AMB_RS11855 at 2552.671 kb on - strand, within AMB_RS11855 at 2552.698 kb on - strand, within AMB_RS11855 at 2552.725 kb on - strand, within AMB_RS11855 at 2552.801 kb on + strand at 2552.926 kb on + strand at 2552.926 kb on + strand at 2553.025 kb on + strand at 2553.080 kb on + strand at 2553.080 kb on + strand at 2553.081 kb on - strand at 2553.081 kb on - strand at 2553.129 kb on - strand at 2553.216 kb on - strand at 2553.230 kb on + strand at 2553.231 kb on - strand at 2553.231 kb on - strand at 2553.231 kb on - strand at 2553.293 kb on + strand, within AMB_RS11860 at 2553.293 kb on + strand, within AMB_RS11860 at 2553.293 kb on + strand, within AMB_RS11860 at 2553.293 kb on + strand, within AMB_RS11860 at 2553.294 kb on - strand, within AMB_RS11860
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 2,550,337 + -0.2 2,550,338 - -0.1 2,550,378 + -0.0 2,550,378 + +0.1 2,550,382 + -0.1 2,550,382 + -0.8 2,550,382 + -0.9 2,550,382 + -1.1 2,550,382 + +0.5 2,550,382 + +1.5 2,552,330 - +0.5 2,552,401 + AMB_RS11855 0.13 -0.0 2,552,401 + AMB_RS11855 0.13 -1.5 2,552,401 + AMB_RS11855 0.13 +0.6 2,552,401 + AMB_RS11855 0.13 -0.1 2,552,402 - AMB_RS11855 0.13 +1.5 2,552,497 + AMB_RS11855 0.34 -0.2 2,552,497 + AMB_RS11855 0.34 +1.5 2,552,497 + AMB_RS11855 0.34 -0.3 2,552,497 + AMB_RS11855 0.34 -1.7 2,552,497 + AMB_RS11855 0.34 -0.4 2,552,498 - AMB_RS11855 0.34 -0.7 2,552,498 - AMB_RS11855 0.34 -1.1 2,552,498 - AMB_RS11855 0.34 -1.5 2,552,498 - AMB_RS11855 0.34 -0.5 2,552,638 + AMB_RS11855 0.65 -2.8 2,552,659 + AMB_RS11855 0.70 +0.3 2,552,671 - AMB_RS11855 0.72 +0.7 2,552,698 - AMB_RS11855 0.78 +0.0 2,552,725 - AMB_RS11855 0.84 -1.0 2,552,801 + -1.1 2,552,926 + +0.1 2,552,926 + -2.1 2,553,025 + -1.1 2,553,080 + -0.3 2,553,080 + +0.3 2,553,081 - -1.9 2,553,081 - -0.8 2,553,129 - -1.1 2,553,216 - +0.3 2,553,230 + -0.1 2,553,231 - +0.2 2,553,231 - -2.0 2,553,231 - -0.1 2,553,293 + AMB_RS11860 0.11 -1.7 2,553,293 + AMB_RS11860 0.11 +0.3 2,553,293 + AMB_RS11860 0.11 -0.1 2,553,293 + AMB_RS11860 0.11 -0.5 2,553,294 - AMB_RS11860 0.11 -0.1
Or see this region's nucleotide sequence