Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS09960 and AMB_RS09965 are separated by 101 nucleotides AMB_RS09965 and AMB_RS09970 overlap by 11 nucleotides AMB_RS09970 and AMB_RS09975 overlap by 4 nucleotides AMB_RS09975 and AMB_RS09980 are separated by 38 nucleotides
AMB_RS09960: AMB_RS09960 - hypothetical protein, at 2,133,028 to 2,134,386
_RS09960
AMB_RS09965: AMB_RS09965 - universal stress protein, at 2,134,488 to 2,135,312
_RS09965
AMB_RS09970: AMB_RS09970 - NAD synthetase, at 2,135,302 to 2,136,143
_RS09970
AMB_RS09975: AMB_RS09975 - ABC transporter permease, at 2,136,140 to 2,137,039
_RS09975
AMB_RS09980: AMB_RS09980 - peptide ABC transporter, at 2,137,078 to 2,138,727
_RS09980
Position (kb)
2135
2136
2137 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2134.381 kb on + strand at 2134.381 kb on + strand at 2134.381 kb on + strand at 2134.382 kb on - strand at 2134.417 kb on - strand at 2134.417 kb on - strand at 2134.448 kb on + strand at 2134.448 kb on + strand at 2134.449 kb on - strand at 2134.449 kb on - strand at 2134.449 kb on - strand at 2134.465 kb on + strand at 2134.465 kb on + strand at 2134.466 kb on - strand at 2134.466 kb on - strand at 2134.494 kb on + strand at 2134.494 kb on + strand at 2134.494 kb on + strand at 2134.495 kb on - strand at 2134.495 kb on - strand at 2134.495 kb on - strand at 2134.495 kb on - strand at 2135.885 kb on - strand, within AMB_RS09970 at 2136.174 kb on + strand at 2136.174 kb on + strand at 2136.273 kb on + strand, within AMB_RS09975 at 2136.273 kb on + strand, within AMB_RS09975 at 2136.273 kb on + strand, within AMB_RS09975 at 2136.274 kb on - strand, within AMB_RS09975 at 2136.450 kb on + strand, within AMB_RS09975 at 2136.450 kb on + strand, within AMB_RS09975 at 2136.451 kb on - strand, within AMB_RS09975 at 2136.451 kb on - strand, within AMB_RS09975 at 2136.544 kb on + strand, within AMB_RS09975 at 2136.545 kb on - strand, within AMB_RS09975 at 2136.545 kb on - strand, within AMB_RS09975 at 2136.840 kb on + strand, within AMB_RS09975 at 2136.840 kb on + strand, within AMB_RS09975 at 2136.841 kb on - strand, within AMB_RS09975 at 2137.023 kb on + strand at 2137.024 kb on - strand at 2137.106 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 2,134,381 + +0.1 2,134,381 + +0.5 2,134,381 + +0.0 2,134,382 - -0.1 2,134,417 - +0.3 2,134,417 - -0.6 2,134,448 + -0.7 2,134,448 + -3.1 2,134,449 - -1.4 2,134,449 - -2.6 2,134,449 - +0.5 2,134,465 + +0.3 2,134,465 + -1.9 2,134,466 - -0.2 2,134,466 - -0.2 2,134,494 + +0.1 2,134,494 + -0.6 2,134,494 + +0.2 2,134,495 - -2.3 2,134,495 - -1.2 2,134,495 - +0.3 2,134,495 - -0.2 2,135,885 - AMB_RS09970 0.69 -0.6 2,136,174 + -0.2 2,136,174 + -1.0 2,136,273 + AMB_RS09975 0.15 -0.0 2,136,273 + AMB_RS09975 0.15 -2.5 2,136,273 + AMB_RS09975 0.15 +0.8 2,136,274 - AMB_RS09975 0.15 +0.2 2,136,450 + AMB_RS09975 0.34 -0.6 2,136,450 + AMB_RS09975 0.34 -1.2 2,136,451 - AMB_RS09975 0.35 +0.2 2,136,451 - AMB_RS09975 0.35 +0.0 2,136,544 + AMB_RS09975 0.45 +0.0 2,136,545 - AMB_RS09975 0.45 -0.4 2,136,545 - AMB_RS09975 0.45 -0.1 2,136,840 + AMB_RS09975 0.78 -1.8 2,136,840 + AMB_RS09975 0.78 -2.8 2,136,841 - AMB_RS09975 0.78 -0.6 2,137,023 + -0.9 2,137,024 - +0.2 2,137,106 + -3.0
Or see this region's nucleotide sequence