Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS09225 and AMB_RS09230 are separated by 49 nucleotides AMB_RS09230 and AMB_RS09235 are separated by 2 nucleotides AMB_RS09235 and AMB_RS23355 overlap by 4 nucleotides
AMB_RS09225: AMB_RS09225 - enolase, at 1,963,051 to 1,964,328
_RS09225
AMB_RS09230: AMB_RS09230 - class I SAM-dependent methyltransferase, at 1,964,378 to 1,965,085
_RS09230
AMB_RS09235: AMB_RS09235 - hypothetical protein, at 1,965,088 to 1,965,948
_RS09235
AMB_RS23355: AMB_RS23355 - two-component sensor histidine kinase, at 1,965,945 to 1,967,540
_RS23355
Position (kb)
1964
1965
1966 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1964.581 kb on - strand, within AMB_RS09230 at 1964.581 kb on - strand, within AMB_RS09230 at 1964.581 kb on - strand, within AMB_RS09230 at 1964.641 kb on - strand, within AMB_RS09230 at 1964.641 kb on - strand, within AMB_RS09230 at 1964.646 kb on + strand, within AMB_RS09230 at 1964.661 kb on + strand, within AMB_RS09230 at 1964.661 kb on + strand, within AMB_RS09230 at 1964.661 kb on + strand, within AMB_RS09230 at 1964.661 kb on + strand, within AMB_RS09230 at 1964.661 kb on + strand, within AMB_RS09230 at 1964.661 kb on + strand, within AMB_RS09230 at 1964.662 kb on - strand, within AMB_RS09230 at 1964.662 kb on - strand, within AMB_RS09230 at 1964.662 kb on - strand, within AMB_RS09230 at 1964.664 kb on + strand, within AMB_RS09230 at 1964.664 kb on + strand, within AMB_RS09230 at 1964.664 kb on + strand, within AMB_RS09230 at 1964.665 kb on - strand, within AMB_RS09230 at 1965.007 kb on - strand, within AMB_RS09230 at 1965.123 kb on - strand at 1965.135 kb on - strand at 1965.147 kb on + strand at 1965.182 kb on + strand, within AMB_RS09235 at 1965.183 kb on - strand, within AMB_RS09235 at 1965.188 kb on + strand, within AMB_RS09235 at 1965.188 kb on + strand, within AMB_RS09235 at 1965.188 kb on + strand, within AMB_RS09235 at 1965.189 kb on - strand, within AMB_RS09235 at 1965.267 kb on + strand, within AMB_RS09235 at 1965.268 kb on - strand, within AMB_RS09235 at 1965.268 kb on - strand, within AMB_RS09235 at 1965.274 kb on + strand, within AMB_RS09235 at 1965.275 kb on - strand, within AMB_RS09235 at 1965.275 kb on - strand, within AMB_RS09235 at 1965.275 kb on - strand, within AMB_RS09235 at 1965.285 kb on + strand, within AMB_RS09235 at 1965.397 kb on + strand, within AMB_RS09235 at 1965.397 kb on + strand, within AMB_RS09235 at 1965.398 kb on - strand, within AMB_RS09235 at 1965.463 kb on + strand, within AMB_RS09235 at 1965.514 kb on + strand, within AMB_RS09235 at 1965.514 kb on + strand, within AMB_RS09235 at 1965.514 kb on + strand, within AMB_RS09235 at 1965.514 kb on + strand, within AMB_RS09235 at 1965.527 kb on - strand, within AMB_RS09235 at 1965.578 kb on + strand, within AMB_RS09235 at 1965.606 kb on - strand, within AMB_RS09235 at 1965.718 kb on + strand, within AMB_RS09235 at 1965.719 kb on - strand, within AMB_RS09235 at 1965.719 kb on - strand, within AMB_RS09235 at 1965.817 kb on + strand, within AMB_RS09235 at 1965.820 kb on + strand, within AMB_RS09235 at 1965.821 kb on - strand, within AMB_RS09235 at 1965.821 kb on - strand, within AMB_RS09235 at 1965.821 kb on - strand, within AMB_RS09235 at 1965.821 kb on - strand, within AMB_RS09235 at 1965.938 kb on - strand at 1966.008 kb on + strand at 1966.008 kb on + strand at 1966.008 kb on + strand at 1966.008 kb on + strand at 1966.008 kb on + strand at 1966.008 kb on + strand at 1966.008 kb on + strand at 1966.009 kb on - strand at 1966.009 kb on - strand at 1966.009 kb on - strand at 1966.009 kb on - strand at 1966.009 kb on - strand at 1966.009 kb on - strand at 1966.009 kb on - strand at 1966.009 kb on - strand at 1966.009 kb on - strand at 1966.009 kb on - strand at 1966.060 kb on + strand at 1966.060 kb on + strand at 1966.061 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 1,964,581 - AMB_RS09230 0.29 +1.4 1,964,581 - AMB_RS09230 0.29 -0.4 1,964,581 - AMB_RS09230 0.29 -0.2 1,964,641 - AMB_RS09230 0.37 +1.2 1,964,641 - AMB_RS09230 0.37 -0.2 1,964,646 + AMB_RS09230 0.38 -2.2 1,964,661 + AMB_RS09230 0.40 -1.2 1,964,661 + AMB_RS09230 0.40 -0.6 1,964,661 + AMB_RS09230 0.40 -0.2 1,964,661 + AMB_RS09230 0.40 +0.4 1,964,661 + AMB_RS09230 0.40 +0.4 1,964,661 + AMB_RS09230 0.40 +0.1 1,964,662 - AMB_RS09230 0.40 +0.3 1,964,662 - AMB_RS09230 0.40 -2.8 1,964,662 - AMB_RS09230 0.40 -0.2 1,964,664 + AMB_RS09230 0.40 +0.4 1,964,664 + AMB_RS09230 0.40 -1.9 1,964,664 + AMB_RS09230 0.40 -0.3 1,964,665 - AMB_RS09230 0.41 +0.4 1,965,007 - AMB_RS09230 0.89 -1.4 1,965,123 - -0.3 1,965,135 - -0.2 1,965,147 + +0.1 1,965,182 + AMB_RS09235 0.11 -1.8 1,965,183 - AMB_RS09235 0.11 -1.1 1,965,188 + AMB_RS09235 0.12 +0.3 1,965,188 + AMB_RS09235 0.12 -0.0 1,965,188 + AMB_RS09235 0.12 -0.1 1,965,189 - AMB_RS09235 0.12 -0.2 1,965,267 + AMB_RS09235 0.21 -1.2 1,965,268 - AMB_RS09235 0.21 -1.2 1,965,268 - AMB_RS09235 0.21 -0.2 1,965,274 + AMB_RS09235 0.22 +0.2 1,965,275 - AMB_RS09235 0.22 +0.0 1,965,275 - AMB_RS09235 0.22 -0.2 1,965,275 - AMB_RS09235 0.22 -0.2 1,965,285 + AMB_RS09235 0.23 -0.2 1,965,397 + AMB_RS09235 0.36 -0.6 1,965,397 + AMB_RS09235 0.36 -0.9 1,965,398 - AMB_RS09235 0.36 -2.5 1,965,463 + AMB_RS09235 0.44 -1.8 1,965,514 + AMB_RS09235 0.49 +0.4 1,965,514 + AMB_RS09235 0.49 +0.3 1,965,514 + AMB_RS09235 0.49 -0.7 1,965,514 + AMB_RS09235 0.49 -1.2 1,965,527 - AMB_RS09235 0.51 -0.5 1,965,578 + AMB_RS09235 0.57 +0.0 1,965,606 - AMB_RS09235 0.60 -0.6 1,965,718 + AMB_RS09235 0.73 +0.0 1,965,719 - AMB_RS09235 0.73 -0.2 1,965,719 - AMB_RS09235 0.73 +0.4 1,965,817 + AMB_RS09235 0.85 -0.3 1,965,820 + AMB_RS09235 0.85 -0.3 1,965,821 - AMB_RS09235 0.85 +0.3 1,965,821 - AMB_RS09235 0.85 -1.4 1,965,821 - AMB_RS09235 0.85 -0.6 1,965,821 - AMB_RS09235 0.85 -0.6 1,965,938 - +0.8 1,966,008 + +0.3 1,966,008 + +0.4 1,966,008 + -0.2 1,966,008 + -0.6 1,966,008 + -0.2 1,966,008 + -2.2 1,966,008 + -0.2 1,966,009 - -0.2 1,966,009 - -1.8 1,966,009 - -0.9 1,966,009 - -1.2 1,966,009 - -2.8 1,966,009 - +0.4 1,966,009 - -1.4 1,966,009 - -3.3 1,966,009 - +0.8 1,966,009 - +0.4 1,966,060 + -0.5 1,966,060 + +0.9 1,966,061 - +0.4
Or see this region's nucleotide sequence