Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS08660 and AMB_RS08665 are separated by 36 nucleotides AMB_RS08665 and AMB_RS08670 are separated by 35 nucleotides AMB_RS08670 and AMB_RS08675 are separated by 25 nucleotides AMB_RS08675 and AMB_RS08680 are separated by 274 nucleotides
AMB_RS08660: AMB_RS08660 - serine protease, at 1,856,263 to 1,857,816
_RS08660
AMB_RS08665: AMB_RS08665 - hypothetical protein, at 1,857,853 to 1,858,074
_RS08665
AMB_RS08670: AMB_RS08670 - hypothetical protein, at 1,858,110 to 1,858,787
_RS08670
AMB_RS08675: AMB_RS08675 - hypothetical protein, at 1,858,813 to 1,859,874
_RS08675
AMB_RS08680: AMB_RS08680 - hypothetical protein, at 1,860,149 to 1,860,901
_RS08680
Position (kb)
1858
1859
1860 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1857.814 kb on + strand at 1857.814 kb on + strand at 1857.814 kb on + strand at 1857.814 kb on + strand at 1857.814 kb on + strand at 1857.814 kb on + strand at 1857.815 kb on - strand at 1857.815 kb on - strand at 1857.815 kb on - strand at 1857.815 kb on - strand at 1857.815 kb on - strand at 1857.815 kb on - strand at 1857.855 kb on - strand at 1857.902 kb on + strand, within AMB_RS08665 at 1857.903 kb on - strand, within AMB_RS08665 at 1857.903 kb on - strand, within AMB_RS08665 at 1858.090 kb on + strand at 1858.091 kb on - strand at 1858.540 kb on - strand, within AMB_RS08670 at 1858.708 kb on + strand, within AMB_RS08670 at 1858.709 kb on - strand, within AMB_RS08670 at 1858.709 kb on - strand, within AMB_RS08670 at 1858.814 kb on + strand at 1858.896 kb on + strand at 1858.897 kb on - strand at 1858.898 kb on + strand at 1858.898 kb on + strand at 1858.898 kb on + strand at 1858.898 kb on + strand at 1858.898 kb on + strand at 1858.898 kb on + strand at 1858.899 kb on - strand at 1858.899 kb on - strand at 1858.899 kb on - strand at 1858.899 kb on - strand at 1858.899 kb on - strand at 1858.899 kb on - strand at 1858.982 kb on + strand, within AMB_RS08675 at 1858.982 kb on + strand, within AMB_RS08675 at 1858.983 kb on - strand, within AMB_RS08675 at 1859.163 kb on - strand, within AMB_RS08675 at 1859.297 kb on + strand, within AMB_RS08675 at 1859.297 kb on + strand, within AMB_RS08675 at 1859.297 kb on + strand, within AMB_RS08675 at 1859.297 kb on + strand, within AMB_RS08675 at 1859.298 kb on - strand, within AMB_RS08675 at 1859.441 kb on + strand, within AMB_RS08675 at 1859.441 kb on + strand, within AMB_RS08675 at 1859.441 kb on + strand, within AMB_RS08675 at 1859.442 kb on - strand, within AMB_RS08675 at 1859.442 kb on - strand, within AMB_RS08675 at 1859.442 kb on - strand, within AMB_RS08675 at 1859.459 kb on + strand, within AMB_RS08675 at 1859.460 kb on - strand, within AMB_RS08675 at 1859.496 kb on - strand, within AMB_RS08675 at 1859.696 kb on + strand, within AMB_RS08675 at 1859.696 kb on + strand, within AMB_RS08675 at 1859.939 kb on + strand at 1859.945 kb on - strand at 1859.953 kb on + strand at 1859.954 kb on - strand at 1859.954 kb on - strand at 1859.954 kb on - strand at 1860.126 kb on - strand at 1860.156 kb on + strand at 1860.331 kb on + strand, within AMB_RS08680 at 1860.331 kb on + strand, within AMB_RS08680 at 1860.375 kb on + strand, within AMB_RS08680 at 1860.376 kb on - strand, within AMB_RS08680 at 1860.505 kb on + strand, within AMB_RS08680 at 1860.590 kb on + strand, within AMB_RS08680 at 1860.620 kb on + strand, within AMB_RS08680 at 1860.621 kb on - strand, within AMB_RS08680 at 1860.662 kb on + strand, within AMB_RS08680 at 1860.663 kb on - strand, within AMB_RS08680 at 1860.663 kb on - strand, within AMB_RS08680 at 1860.704 kb on - strand, within AMB_RS08680 at 1860.704 kb on - strand, within AMB_RS08680 at 1860.809 kb on + strand, within AMB_RS08680 at 1860.869 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 1,857,814 + -1.4 1,857,814 + -1.2 1,857,814 + -0.2 1,857,814 + -0.8 1,857,814 + -0.5 1,857,814 + -2.5 1,857,815 - -2.3 1,857,815 - -0.6 1,857,815 - -0.6 1,857,815 - -0.0 1,857,815 - -1.4 1,857,815 - -2.8 1,857,855 - -0.2 1,857,902 + AMB_RS08665 0.22 +0.3 1,857,903 - AMB_RS08665 0.23 +0.2 1,857,903 - AMB_RS08665 0.23 -0.3 1,858,090 + -0.3 1,858,091 - +0.5 1,858,540 - AMB_RS08670 0.63 +0.0 1,858,708 + AMB_RS08670 0.88 +0.1 1,858,709 - AMB_RS08670 0.88 +0.2 1,858,709 - AMB_RS08670 0.88 -1.6 1,858,814 + -0.6 1,858,896 + -2.4 1,858,897 - -0.1 1,858,898 + +0.7 1,858,898 + +0.2 1,858,898 + -1.1 1,858,898 + +1.1 1,858,898 + +0.4 1,858,898 + -1.2 1,858,899 - -1.2 1,858,899 - +0.3 1,858,899 - -0.2 1,858,899 - -0.2 1,858,899 - +0.4 1,858,899 - -2.1 1,858,982 + AMB_RS08675 0.16 +0.4 1,858,982 + AMB_RS08675 0.16 +0.4 1,858,983 - AMB_RS08675 0.16 +0.5 1,859,163 - AMB_RS08675 0.33 -1.1 1,859,297 + AMB_RS08675 0.46 +0.4 1,859,297 + AMB_RS08675 0.46 +0.4 1,859,297 + AMB_RS08675 0.46 -2.8 1,859,297 + AMB_RS08675 0.46 +0.4 1,859,298 - AMB_RS08675 0.46 +0.4 1,859,441 + AMB_RS08675 0.59 +0.6 1,859,441 + AMB_RS08675 0.59 +1.1 1,859,441 + AMB_RS08675 0.59 -0.2 1,859,442 - AMB_RS08675 0.59 +0.7 1,859,442 - AMB_RS08675 0.59 -1.2 1,859,442 - AMB_RS08675 0.59 -0.9 1,859,459 + AMB_RS08675 0.61 +0.1 1,859,460 - AMB_RS08675 0.61 +0.2 1,859,496 - AMB_RS08675 0.64 +0.1 1,859,696 + AMB_RS08675 0.83 +0.3 1,859,696 + AMB_RS08675 0.83 -0.9 1,859,939 + +0.2 1,859,945 - -1.2 1,859,953 + -0.6 1,859,954 - +0.3 1,859,954 - -0.3 1,859,954 - -1.9 1,860,126 - -0.2 1,860,156 + +0.2 1,860,331 + AMB_RS08680 0.24 -1.4 1,860,331 + AMB_RS08680 0.24 +0.7 1,860,375 + AMB_RS08680 0.30 +0.3 1,860,376 - AMB_RS08680 0.30 -0.2 1,860,505 + AMB_RS08680 0.47 -0.9 1,860,590 + AMB_RS08680 0.59 -0.1 1,860,620 + AMB_RS08680 0.63 -2.2 1,860,621 - AMB_RS08680 0.63 -0.4 1,860,662 + AMB_RS08680 0.68 -0.1 1,860,663 - AMB_RS08680 0.68 +0.3 1,860,663 - AMB_RS08680 0.68 +0.1 1,860,704 - AMB_RS08680 0.74 -0.9 1,860,704 - AMB_RS08680 0.74 -0.2 1,860,809 + AMB_RS08680 0.88 -3.1 1,860,869 - -0.2
Or see this region's nucleotide sequence