Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS08045 and AMB_RS08050 are separated by 155 nucleotides AMB_RS08050 and AMB_RS08055 are separated by 164 nucleotides AMB_RS08055 and AMB_RS08060 are separated by 6 nucleotides AMB_RS08060 and AMB_RS08065 are separated by 64 nucleotides
AMB_RS08045: AMB_RS08045 - hypothetical protein, at 1,722,274 to 1,722,780
_RS08045
AMB_RS08050: AMB_RS08050 - rhodanese-like domain-containing protein, at 1,722,936 to 1,723,271
_RS08050
AMB_RS08055: AMB_RS08055 - hypothetical protein, at 1,723,436 to 1,723,891
_RS08055
AMB_RS08060: AMB_RS08060 - RNA polymerase sigma factor, at 1,723,898 to 1,724,413
_RS08060
AMB_RS08065: AMB_RS08065 - serine protease, at 1,724,478 to 1,726,226
_RS08065
Position (kb)
1723
1724 Strain fitness (log2 ratio)
-2
-1
0
1 at 1722.581 kb on + strand, within AMB_RS08045 at 1722.581 kb on + strand, within AMB_RS08045 at 1722.581 kb on + strand, within AMB_RS08045 at 1722.581 kb on + strand, within AMB_RS08045 at 1722.582 kb on - strand, within AMB_RS08045 at 1722.582 kb on - strand, within AMB_RS08045 at 1722.584 kb on + strand, within AMB_RS08045 at 1722.584 kb on + strand, within AMB_RS08045 at 1722.585 kb on - strand, within AMB_RS08045 at 1722.585 kb on - strand, within AMB_RS08045 at 1722.771 kb on + strand at 1722.828 kb on - strand at 1722.916 kb on - strand at 1723.056 kb on - strand, within AMB_RS08050 at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.289 kb on + strand at 1723.290 kb on - strand at 1723.290 kb on - strand at 1723.358 kb on - strand at 1723.358 kb on - strand at 1723.358 kb on - strand at 1723.362 kb on - strand at 1723.362 kb on - strand at 1723.376 kb on + strand at 1723.376 kb on + strand at 1723.377 kb on - strand at 1723.377 kb on - strand at 1724.096 kb on + strand, within AMB_RS08060 at 1724.096 kb on + strand, within AMB_RS08060 at 1724.096 kb on + strand, within AMB_RS08060 at 1724.096 kb on + strand, within AMB_RS08060 at 1724.097 kb on - strand, within AMB_RS08060 at 1724.451 kb on + strand at 1724.484 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 1,722,581 + AMB_RS08045 0.61 -2.1 1,722,581 + AMB_RS08045 0.61 -1.1 1,722,581 + AMB_RS08045 0.61 -1.6 1,722,581 + AMB_RS08045 0.61 -0.1 1,722,582 - AMB_RS08045 0.61 -0.2 1,722,582 - AMB_RS08045 0.61 +0.3 1,722,584 + AMB_RS08045 0.61 -0.8 1,722,584 + AMB_RS08045 0.61 -0.6 1,722,585 - AMB_RS08045 0.61 -0.1 1,722,585 - AMB_RS08045 0.61 -1.4 1,722,771 + -1.6 1,722,828 - +0.0 1,722,916 - +0.2 1,723,056 - AMB_RS08050 0.36 +0.2 1,723,289 + -1.9 1,723,289 + -2.1 1,723,289 + +0.0 1,723,289 + +0.3 1,723,289 + -0.6 1,723,289 + +0.5 1,723,289 + -0.7 1,723,289 + +0.4 1,723,290 - -0.2 1,723,290 - +0.5 1,723,358 - +0.3 1,723,358 - -0.2 1,723,358 - +0.1 1,723,362 - -1.4 1,723,362 - -0.0 1,723,376 + -0.2 1,723,376 + -1.9 1,723,377 - -1.4 1,723,377 - -0.5 1,724,096 + AMB_RS08060 0.38 -0.8 1,724,096 + AMB_RS08060 0.38 +0.3 1,724,096 + AMB_RS08060 0.38 -1.3 1,724,096 + AMB_RS08060 0.38 -1.2 1,724,097 - AMB_RS08060 0.39 -0.4 1,724,451 + -1.6 1,724,484 - -2.1
Or see this region's nucleotide sequence