Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS06335 and AMB_RS06340 are separated by 5 nucleotides AMB_RS06340 and AMB_RS06345 are separated by 51 nucleotides AMB_RS06345 and AMB_RS06350 are separated by 101 nucleotides AMB_RS06350 and AMB_RS06355 are separated by 76 nucleotides
AMB_RS06335: AMB_RS06335 - CusA/CzcA family heavy metal efflux RND transporter, at 1,329,527 to 1,332,628
_RS06335
AMB_RS06340: AMB_RS06340 - efflux RND transporter periplasmic adaptor subunit, at 1,332,634 to 1,334,190
_RS06340
AMB_RS06345: AMB_RS06345 - hypothetical protein, at 1,334,242 to 1,334,616
_RS06345
AMB_RS06350: AMB_RS06350 - PilZ domain-containing protein, at 1,334,718 to 1,335,104
_RS06350
AMB_RS06355: AMB_RS06355 - PAS domain-containing sensor histidine kinase, at 1,335,181 to 1,336,341
_RS06355
Position (kb)
1332
1333
1334
1335 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1332.021 kb on + strand, within AMB_RS06335 at 1332.021 kb on + strand, within AMB_RS06335 at 1332.021 kb on + strand, within AMB_RS06335 at 1332.137 kb on + strand, within AMB_RS06335 at 1332.137 kb on + strand, within AMB_RS06335 at 1332.137 kb on + strand, within AMB_RS06335 at 1332.137 kb on + strand, within AMB_RS06335 at 1332.138 kb on - strand, within AMB_RS06335 at 1332.138 kb on - strand, within AMB_RS06335 at 1332.138 kb on - strand, within AMB_RS06335 at 1332.318 kb on + strand at 1332.319 kb on - strand at 1332.319 kb on - strand at 1332.319 kb on - strand at 1332.552 kb on + strand at 1332.553 kb on - strand at 1332.635 kb on + strand at 1332.997 kb on + strand, within AMB_RS06340 at 1332.997 kb on + strand, within AMB_RS06340 at 1332.998 kb on - strand, within AMB_RS06340 at 1332.998 kb on - strand, within AMB_RS06340 at 1333.938 kb on - strand, within AMB_RS06340 at 1334.043 kb on - strand at 1334.043 kb on - strand at 1334.698 kb on + strand at 1334.698 kb on + strand at 1334.722 kb on + strand at 1334.928 kb on + strand, within AMB_RS06350 at 1334.929 kb on - strand, within AMB_RS06350 at 1334.929 kb on - strand, within AMB_RS06350 at 1334.929 kb on - strand, within AMB_RS06350 at 1335.036 kb on + strand, within AMB_RS06350 at 1335.037 kb on - strand, within AMB_RS06350 at 1335.037 kb on - strand, within AMB_RS06350 at 1335.093 kb on + strand at 1335.093 kb on + strand at 1335.093 kb on + strand at 1335.093 kb on + strand at 1335.094 kb on - strand at 1335.102 kb on + strand at 1335.169 kb on + strand at 1335.169 kb on + strand at 1335.169 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 1,332,021 + AMB_RS06335 0.80 +0.5 1,332,021 + AMB_RS06335 0.80 -1.4 1,332,021 + AMB_RS06335 0.80 +0.3 1,332,137 + AMB_RS06335 0.84 -0.6 1,332,137 + AMB_RS06335 0.84 +0.8 1,332,137 + AMB_RS06335 0.84 -0.2 1,332,137 + AMB_RS06335 0.84 -1.4 1,332,138 - AMB_RS06335 0.84 -0.0 1,332,138 - AMB_RS06335 0.84 +0.4 1,332,138 - AMB_RS06335 0.84 +0.1 1,332,318 + -0.4 1,332,319 - +0.1 1,332,319 - -0.6 1,332,319 - +0.6 1,332,552 + -1.7 1,332,553 - -1.6 1,332,635 + -1.1 1,332,997 + AMB_RS06340 0.23 -0.1 1,332,997 + AMB_RS06340 0.23 -1.1 1,332,998 - AMB_RS06340 0.23 -0.4 1,332,998 - AMB_RS06340 0.23 +0.1 1,333,938 - AMB_RS06340 0.84 +0.2 1,334,043 - -0.1 1,334,043 - +0.3 1,334,698 + +0.2 1,334,698 + +0.3 1,334,722 + +0.8 1,334,928 + AMB_RS06350 0.54 +0.3 1,334,929 - AMB_RS06350 0.55 -1.7 1,334,929 - AMB_RS06350 0.55 +0.4 1,334,929 - AMB_RS06350 0.55 -3.7 1,335,036 + AMB_RS06350 0.82 +0.1 1,335,037 - AMB_RS06350 0.82 +0.4 1,335,037 - AMB_RS06350 0.82 -1.4 1,335,093 + -0.5 1,335,093 + +0.3 1,335,093 + +0.1 1,335,093 + +1.4 1,335,094 - -1.4 1,335,102 + -0.3 1,335,169 + -2.1 1,335,169 + -0.3 1,335,169 + -0.9
Or see this region's nucleotide sequence