Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS06000 and AMB_RS24010 are separated by 3 nucleotides AMB_RS24010 and AMB_RS06005 are separated by 38 nucleotides AMB_RS06005 and AMB_RS06010 are separated by 6 nucleotides AMB_RS06010 and AMB_RS06015 are separated by 11 nucleotides
AMB_RS06000: AMB_RS06000 - replication protein, at 1,256,618 to 1,259,608
_RS06000
AMB_RS24010: AMB_RS24010 - hypothetical protein, at 1,259,612 to 1,259,851
_RS24010
AMB_RS06005: AMB_RS06005 - hypothetical protein, at 1,259,890 to 1,260,387
_RS06005
AMB_RS06010: AMB_RS06010 - hypothetical protein, at 1,260,394 to 1,261,263
_RS06010
AMB_RS06015: AMB_RS06015 - hypothetical protein, at 1,261,275 to 1,261,685
_RS06015
Position (kb)
1259
1260
1261 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1259.270 kb on + strand, within AMB_RS06000 at 1259.600 kb on + strand at 1259.606 kb on + strand at 1259.682 kb on + strand, within AMB_RS24010 at 1259.683 kb on - strand, within AMB_RS24010 at 1259.748 kb on + strand, within AMB_RS24010 at 1259.748 kb on + strand, within AMB_RS24010 at 1259.748 kb on + strand, within AMB_RS24010 at 1259.862 kb on + strand at 1259.862 kb on + strand at 1259.862 kb on + strand at 1259.863 kb on - strand at 1259.936 kb on - strand at 1259.936 kb on - strand at 1260.200 kb on - strand, within AMB_RS06005 at 1260.200 kb on - strand, within AMB_RS06005 at 1260.200 kb on - strand, within AMB_RS06005 at 1260.200 kb on - strand, within AMB_RS06005 at 1260.968 kb on + strand, within AMB_RS06010
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 1,259,270 + AMB_RS06000 0.89 -0.9 1,259,600 + -1.1 1,259,606 + -0.1 1,259,682 + AMB_RS24010 0.29 -0.4 1,259,683 - AMB_RS24010 0.30 +0.0 1,259,748 + AMB_RS24010 0.57 +0.5 1,259,748 + AMB_RS24010 0.57 +0.9 1,259,748 + AMB_RS24010 0.57 -1.9 1,259,862 + -1.5 1,259,862 + +0.3 1,259,862 + -3.7 1,259,863 - -0.5 1,259,936 - -0.1 1,259,936 - -2.2 1,260,200 - AMB_RS06005 0.62 -2.5 1,260,200 - AMB_RS06005 0.62 -2.1 1,260,200 - AMB_RS06005 0.62 -1.7 1,260,200 - AMB_RS06005 0.62 -0.3 1,260,968 + AMB_RS06010 0.66 -2.9
Or see this region's nucleotide sequence