Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04330 and AMB_RS04335 are separated by 87 nucleotides AMB_RS04335 and AMB_RS04340 are separated by 70 nucleotides AMB_RS04340 and AMB_RS04345 are separated by 11 nucleotides AMB_RS04345 and AMB_RS04350 are separated by 67 nucleotides
AMB_RS04330: AMB_RS04330 - xanthine and CO dehydrogenase maturation factor, at 911,130 to 911,909
_RS04330
AMB_RS04335: AMB_RS04335 - PaaI family thioesterase, at 911,997 to 912,407
_RS04335
AMB_RS04340: AMB_RS04340 - enoyl-CoA hydratase, at 912,478 to 913,263
_RS04340
AMB_RS04345: AMB_RS04345 - anti-sigma factor antagonist, at 913,275 to 913,577
_RS04345
AMB_RS04350: AMB_RS04350 - HAMP domain-containing protein, at 913,645 to 915,693
_RS04350
Position (kb)
912
913
914 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 911.643 kb on + strand, within AMB_RS04330 at 911.644 kb on - strand, within AMB_RS04330 at 911.908 kb on - strand at 911.908 kb on - strand at 911.986 kb on - strand at 911.986 kb on - strand at 911.986 kb on - strand at 911.986 kb on - strand at 912.010 kb on + strand at 912.010 kb on + strand at 912.010 kb on + strand at 912.010 kb on + strand at 912.011 kb on - strand at 912.211 kb on + strand, within AMB_RS04335 at 912.353 kb on + strand, within AMB_RS04335 at 912.421 kb on + strand at 912.477 kb on + strand at 912.477 kb on + strand at 912.477 kb on + strand at 912.477 kb on + strand at 912.478 kb on - strand at 913.123 kb on + strand, within AMB_RS04340 at 913.123 kb on + strand, within AMB_RS04340 at 913.124 kb on - strand, within AMB_RS04340 at 913.124 kb on - strand, within AMB_RS04340 at 913.124 kb on - strand, within AMB_RS04340 at 913.156 kb on + strand, within AMB_RS04340 at 913.188 kb on + strand at 913.188 kb on + strand at 913.189 kb on - strand at 913.189 kb on - strand at 913.276 kb on + strand at 913.384 kb on + strand, within AMB_RS04345 at 913.384 kb on + strand, within AMB_RS04345 at 913.384 kb on + strand, within AMB_RS04345 at 913.384 kb on + strand, within AMB_RS04345 at 913.384 kb on + strand, within AMB_RS04345 at 913.384 kb on + strand, within AMB_RS04345 at 913.385 kb on - strand, within AMB_RS04345 at 913.385 kb on - strand, within AMB_RS04345 at 913.385 kb on - strand, within AMB_RS04345 at 913.385 kb on - strand, within AMB_RS04345 at 913.385 kb on - strand, within AMB_RS04345 at 913.385 kb on - strand, within AMB_RS04345 at 913.385 kb on - strand, within AMB_RS04345 at 913.385 kb on - strand, within AMB_RS04345 at 913.570 kb on + strand at 913.570 kb on + strand at 913.570 kb on + strand at 913.571 kb on - strand at 913.571 kb on - strand at 913.574 kb on - strand at 913.639 kb on + strand at 913.639 kb on + strand at 913.639 kb on + strand at 913.639 kb on + strand at 913.639 kb on + strand at 913.639 kb on + strand at 913.639 kb on + strand at 913.639 kb on + strand at 913.640 kb on - strand at 913.640 kb on - strand at 913.825 kb on + strand at 913.826 kb on - strand at 913.826 kb on - strand at 913.891 kb on + strand, within AMB_RS04350 at 913.891 kb on + strand, within AMB_RS04350 at 914.122 kb on + strand, within AMB_RS04350 at 914.122 kb on + strand, within AMB_RS04350 at 914.123 kb on - strand, within AMB_RS04350
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 911,643 + AMB_RS04330 0.66 -1.3 911,644 - AMB_RS04330 0.66 -0.5 911,908 - +0.2 911,908 - -2.1 911,986 - -1.1 911,986 - +0.1 911,986 - -0.1 911,986 - -1.7 912,010 + +0.3 912,010 + -0.1 912,010 + -1.7 912,010 + -0.2 912,011 - -3.7 912,211 + AMB_RS04335 0.52 +0.5 912,353 + AMB_RS04335 0.87 +1.5 912,421 + +0.4 912,477 + -0.0 912,477 + -0.8 912,477 + +0.9 912,477 + -0.6 912,478 - -0.9 913,123 + AMB_RS04340 0.82 -0.0 913,123 + AMB_RS04340 0.82 -0.8 913,124 - AMB_RS04340 0.82 -1.7 913,124 - AMB_RS04340 0.82 -1.1 913,124 - AMB_RS04340 0.82 +0.0 913,156 + AMB_RS04340 0.86 +0.1 913,188 + -1.2 913,188 + -1.2 913,189 - +0.2 913,189 - -2.0 913,276 + -2.2 913,384 + AMB_RS04345 0.36 +0.8 913,384 + AMB_RS04345 0.36 -2.3 913,384 + AMB_RS04345 0.36 -1.5 913,384 + AMB_RS04345 0.36 -2.7 913,384 + AMB_RS04345 0.36 +0.3 913,384 + AMB_RS04345 0.36 -2.2 913,385 - AMB_RS04345 0.36 +1.5 913,385 - AMB_RS04345 0.36 -0.2 913,385 - AMB_RS04345 0.36 -1.0 913,385 - AMB_RS04345 0.36 -0.5 913,385 - AMB_RS04345 0.36 -0.4 913,385 - AMB_RS04345 0.36 -2.3 913,385 - AMB_RS04345 0.36 -2.1 913,385 - AMB_RS04345 0.36 -0.1 913,570 + +0.4 913,570 + +0.1 913,570 + -1.1 913,571 - -0.9 913,571 - -2.6 913,574 - +0.5 913,639 + +0.3 913,639 + +0.0 913,639 + -0.1 913,639 + -0.2 913,639 + -1.8 913,639 + -0.1 913,639 + -2.1 913,639 + -1.1 913,640 - -2.5 913,640 - +0.5 913,825 + +0.1 913,826 - -2.3 913,826 - -1.1 913,891 + AMB_RS04350 0.12 +0.1 913,891 + AMB_RS04350 0.12 -0.1 914,122 + AMB_RS04350 0.23 -0.1 914,122 + AMB_RS04350 0.23 +0.1 914,123 - AMB_RS04350 0.23 -2.0
Or see this region's nucleotide sequence