Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04220 and AMB_RS04225 are separated by 105 nucleotides AMB_RS04225 and AMB_RS04230 are separated by 20 nucleotides AMB_RS04230 and AMB_RS04235 are separated by 109 nucleotides
AMB_RS04220: AMB_RS04220 - transcriptional regulator GcvA, at 887,667 to 888,557
_RS04220
AMB_RS04225: AMB_RS04225 - EamA/RhaT family transporter, at 888,663 to 889,553
_RS04225
AMB_RS04230: AMB_RS04230 - acyl-CoA thioesterase, at 889,574 to 890,011
_RS04230
AMB_RS04235: AMB_RS04235 - hypothetical protein, at 890,121 to 890,567
_RS04235
Position (kb) 888
889
890 Strain fitness (log2 ratio) -2
-1
0
1 at 887.755 kb on + strand at 887.755 kb on + strand at 887.756 kb on - strand, within AMB_RS04220 at 888.081 kb on + strand, within AMB_RS04220 at 888.681 kb on + strand at 888.681 kb on + strand at 888.681 kb on + strand at 889.164 kb on + strand, within AMB_RS04225 at 889.350 kb on + strand, within AMB_RS04225 at 889.350 kb on + strand, within AMB_RS04225 at 889.350 kb on + strand, within AMB_RS04225 at 889.351 kb on - strand, within AMB_RS04225 at 889.351 kb on - strand, within AMB_RS04225 at 889.351 kb on - strand, within AMB_RS04225 at 889.510 kb on + strand at 889.510 kb on + strand at 889.510 kb on + strand at 889.510 kb on + strand at 889.511 kb on - strand at 889.511 kb on - strand at 889.607 kb on + strand at 889.608 kb on - strand at 889.608 kb on - strand at 889.637 kb on + strand, within AMB_RS04230 at 889.638 kb on - strand, within AMB_RS04230 at 889.664 kb on - strand, within AMB_RS04230 at 889.835 kb on - strand, within AMB_RS04230 at 890.024 kb on + strand at 890.024 kb on + strand at 890.024 kb on + strand at 890.024 kb on + strand at 890.024 kb on + strand at 890.024 kb on + strand at 890.025 kb on - strand at 890.068 kb on + strand at 890.069 kb on - strand at 890.176 kb on + strand, within AMB_RS04235 at 890.177 kb on - strand, within AMB_RS04235 at 890.182 kb on + strand, within AMB_RS04235 at 890.182 kb on + strand, within AMB_RS04235 at 890.182 kb on + strand, within AMB_RS04235 at 890.183 kb on - strand, within AMB_RS04235 at 890.183 kb on - strand, within AMB_RS04235 at 890.183 kb on - strand, within AMB_RS04235 at 890.312 kb on - strand, within AMB_RS04235 at 890.396 kb on - strand, within AMB_RS04235
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 887,755 + -1.8 887,755 + -1.5 887,756 - AMB_RS04220 0.10 -0.1 888,081 + AMB_RS04220 0.46 -1.2 888,681 + -1.0 888,681 + +0.5 888,681 + +0.2 889,164 + AMB_RS04225 0.56 -1.5 889,350 + AMB_RS04225 0.77 +0.3 889,350 + AMB_RS04225 0.77 -0.6 889,350 + AMB_RS04225 0.77 +0.9 889,351 - AMB_RS04225 0.77 -2.1 889,351 - AMB_RS04225 0.77 -0.1 889,351 - AMB_RS04225 0.77 -0.1 889,510 + +0.9 889,510 + +0.5 889,510 + +0.4 889,510 + +0.5 889,511 - +0.5 889,511 - +0.2 889,607 + -2.0 889,608 - -1.3 889,608 - +0.6 889,637 + AMB_RS04230 0.14 +0.9 889,638 - AMB_RS04230 0.15 -0.1 889,664 - AMB_RS04230 0.21 +0.3 889,835 - AMB_RS04230 0.60 +0.2 890,024 + -0.5 890,024 + +0.3 890,024 + -0.1 890,024 + +0.4 890,024 + -0.5 890,024 + -0.8 890,025 - -0.1 890,068 + -0.0 890,069 - +0.2 890,176 + AMB_RS04235 0.12 -0.8 890,177 - AMB_RS04235 0.13 +1.5 890,182 + AMB_RS04235 0.14 +0.1 890,182 + AMB_RS04235 0.14 -0.1 890,182 + AMB_RS04235 0.14 -1.1 890,183 - AMB_RS04235 0.14 -1.7 890,183 - AMB_RS04235 0.14 -1.5 890,183 - AMB_RS04235 0.14 +0.5 890,312 - AMB_RS04235 0.43 +1.5 890,396 - AMB_RS04235 0.62 +0.0
Or see this region's nucleotide sequence