Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03390 and AMB_RS03395 are separated by 8 nucleotides AMB_RS03395 and AMB_RS03400 are separated by 9 nucleotides
AMB_RS03390: AMB_RS03390 - XRE family transcriptional regulator, at 712,256 to 712,492
_RS03390
AMB_RS03395: AMB_RS03395 - addiction module antidote protein, HigA family, at 712,501 to 712,788
_RS03395
AMB_RS03400: AMB_RS03400 - plasmid maintenance system killer protein, at 712,798 to 713,076
_RS03400
Position (kb) 712
713 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 711.296 kb on + strand at 711.296 kb on + strand at 711.297 kb on - strand at 711.297 kb on - strand at 711.297 kb on - strand at 711.297 kb on - strand at 711.330 kb on - strand at 711.370 kb on + strand at 711.658 kb on - strand at 711.659 kb on + strand at 711.660 kb on - strand at 711.660 kb on - strand at 711.692 kb on + strand at 711.770 kb on - strand at 711.770 kb on - strand at 711.906 kb on + strand at 711.939 kb on - strand at 712.000 kb on - strand at 712.112 kb on + strand at 712.129 kb on - strand at 712.129 kb on - strand at 712.129 kb on - strand at 712.161 kb on + strand at 712.162 kb on - strand at 712.237 kb on + strand at 712.237 kb on + strand at 712.333 kb on + strand, within AMB_RS03390 at 712.376 kb on + strand, within AMB_RS03390 at 712.377 kb on - strand, within AMB_RS03390 at 712.377 kb on - strand, within AMB_RS03390 at 712.388 kb on + strand, within AMB_RS03390 at 712.388 kb on + strand, within AMB_RS03390 at 712.388 kb on + strand, within AMB_RS03390 at 712.388 kb on + strand, within AMB_RS03390 at 712.389 kb on - strand, within AMB_RS03390 at 712.389 kb on - strand, within AMB_RS03390 at 712.389 kb on - strand, within AMB_RS03390 at 712.491 kb on - strand at 712.502 kb on + strand at 712.806 kb on - strand at 712.806 kb on - strand at 712.847 kb on - strand, within AMB_RS03400 at 712.847 kb on - strand, within AMB_RS03400 at 712.847 kb on - strand, within AMB_RS03400 at 712.849 kb on - strand, within AMB_RS03400 at 712.849 kb on - strand, within AMB_RS03400 at 713.037 kb on - strand, within AMB_RS03400 at 713.037 kb on - strand, within AMB_RS03400 at 713.039 kb on - strand, within AMB_RS03400 at 713.039 kb on - strand, within AMB_RS03400 at 713.088 kb on - strand at 713.104 kb on - strand at 713.168 kb on + strand at 713.169 kb on - strand at 713.298 kb on - strand at 713.393 kb on + strand at 713.402 kb on - strand at 713.468 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 711,296 + -0.6 711,296 + -0.5 711,297 - -0.1 711,297 - +0.6 711,297 - +0.0 711,297 - -1.1 711,330 - +0.1 711,370 + -2.8 711,658 - -0.3 711,659 + -0.1 711,660 - -0.5 711,660 - -1.5 711,692 + -0.5 711,770 - -0.3 711,770 - -0.1 711,906 + -0.2 711,939 - -0.5 712,000 - -1.8 712,112 + +0.7 712,129 - +1.5 712,129 - -0.4 712,129 - -0.1 712,161 + -1.1 712,162 - -0.3 712,237 + -2.1 712,237 + -1.9 712,333 + AMB_RS03390 0.32 -1.1 712,376 + AMB_RS03390 0.51 +0.2 712,377 - AMB_RS03390 0.51 -1.1 712,377 - AMB_RS03390 0.51 -0.8 712,388 + AMB_RS03390 0.56 +0.4 712,388 + AMB_RS03390 0.56 +0.4 712,388 + AMB_RS03390 0.56 -0.2 712,388 + AMB_RS03390 0.56 +0.1 712,389 - AMB_RS03390 0.56 +0.5 712,389 - AMB_RS03390 0.56 +0.2 712,389 - AMB_RS03390 0.56 +0.6 712,491 - -0.2 712,502 + +0.7 712,806 - +0.2 712,806 - -2.6 712,847 - AMB_RS03400 0.18 +0.3 712,847 - AMB_RS03400 0.18 -3.1 712,847 - AMB_RS03400 0.18 -1.5 712,849 - AMB_RS03400 0.18 -0.7 712,849 - AMB_RS03400 0.18 +0.7 713,037 - AMB_RS03400 0.86 +0.5 713,037 - AMB_RS03400 0.86 +0.2 713,039 - AMB_RS03400 0.86 -1.8 713,039 - AMB_RS03400 0.86 -1.5 713,088 - -1.5 713,104 - -0.6 713,168 + +0.2 713,169 - +0.7 713,298 - +2.0 713,393 + -1.1 713,402 - -1.9 713,468 - -1.9
Or see this region's nucleotide sequence