Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS02510 and AMB_RS02515 are separated by 222 nucleotides AMB_RS02515 and AMB_RS02520 are separated by 48 nucleotides AMB_RS02520 and AMB_RS02525 are separated by 111 nucleotides
AMB_RS02510: AMB_RS02510 - DUF4102 domain-containing protein, at 519,546 to 520,784
_RS02510
AMB_RS02515: AMB_RS02515 - tRNA-Ala, at 521,007 to 521,082
_RS02515
AMB_RS02520: AMB_RS02520 - hypothetical protein, at 521,131 to 522,141
_RS02520
AMB_RS02525: AMB_RS02525 - U32 family peptidase, at 522,253 to 523,158
_RS02525
Position (kb) 521
522
523 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 520.255 kb on + strand, within AMB_RS02510 at 520.262 kb on + strand, within AMB_RS02510 at 520.263 kb on - strand, within AMB_RS02510 at 520.263 kb on - strand, within AMB_RS02510 at 520.263 kb on - strand, within AMB_RS02510 at 520.263 kb on - strand, within AMB_RS02510 at 520.289 kb on + strand, within AMB_RS02510 at 520.355 kb on + strand, within AMB_RS02510 at 520.356 kb on - strand, within AMB_RS02510 at 520.356 kb on - strand, within AMB_RS02510 at 520.457 kb on + strand, within AMB_RS02510 at 520.458 kb on - strand, within AMB_RS02510 at 520.458 kb on - strand, within AMB_RS02510 at 520.560 kb on + strand, within AMB_RS02510 at 520.561 kb on - strand, within AMB_RS02510 at 520.661 kb on - strand at 520.675 kb on + strand at 520.676 kb on - strand at 520.732 kb on - strand at 520.732 kb on - strand at 520.732 kb on - strand at 520.760 kb on + strand at 520.793 kb on - strand at 520.814 kb on - strand at 520.814 kb on - strand at 520.827 kb on + strand at 520.827 kb on + strand at 520.827 kb on + strand at 520.827 kb on + strand at 520.828 kb on - strand at 520.828 kb on - strand at 520.828 kb on - strand at 520.899 kb on + strand at 520.900 kb on - strand at 520.900 kb on - strand at 520.949 kb on - strand at 521.214 kb on - strand at 521.282 kb on - strand, within AMB_RS02520 at 521.357 kb on + strand, within AMB_RS02520 at 521.357 kb on + strand, within AMB_RS02520 at 521.358 kb on - strand, within AMB_RS02520 at 521.358 kb on - strand, within AMB_RS02520 at 521.429 kb on + strand, within AMB_RS02520 at 521.429 kb on + strand, within AMB_RS02520 at 521.429 kb on + strand, within AMB_RS02520 at 521.430 kb on - strand, within AMB_RS02520 at 521.510 kb on + strand, within AMB_RS02520 at 521.510 kb on + strand, within AMB_RS02520 at 521.510 kb on + strand, within AMB_RS02520 at 521.511 kb on - strand, within AMB_RS02520 at 521.511 kb on - strand, within AMB_RS02520 at 521.511 kb on - strand, within AMB_RS02520 at 521.583 kb on + strand, within AMB_RS02520 at 521.585 kb on + strand, within AMB_RS02520 at 521.586 kb on - strand, within AMB_RS02520 at 521.615 kb on + strand, within AMB_RS02520 at 521.615 kb on + strand, within AMB_RS02520 at 521.798 kb on + strand, within AMB_RS02520 at 521.798 kb on + strand, within AMB_RS02520 at 521.799 kb on - strand, within AMB_RS02520 at 521.799 kb on - strand, within AMB_RS02520 at 522.050 kb on + strand at 522.050 kb on + strand at 522.051 kb on - strand at 522.219 kb on + strand at 522.219 kb on + strand at 522.219 kb on + strand at 522.219 kb on + strand at 522.219 kb on + strand at 522.219 kb on + strand at 522.219 kb on + strand at 522.299 kb on + strand at 522.299 kb on + strand at 522.300 kb on - strand at 522.300 kb on - strand at 522.300 kb on - strand at 522.300 kb on - strand at 522.300 kb on - strand at 522.497 kb on + strand, within AMB_RS02525 at 522.497 kb on + strand, within AMB_RS02525 at 522.498 kb on - strand, within AMB_RS02525 at 522.498 kb on - strand, within AMB_RS02525 at 522.588 kb on - strand, within AMB_RS02525 at 522.674 kb on - strand, within AMB_RS02525 at 522.674 kb on - strand, within AMB_RS02525 at 522.674 kb on - strand, within AMB_RS02525 at 522.674 kb on - strand, within AMB_RS02525 at 522.674 kb on - strand, within AMB_RS02525 at 522.794 kb on + strand, within AMB_RS02525 at 522.794 kb on + strand, within AMB_RS02525 at 522.794 kb on + strand, within AMB_RS02525 at 522.794 kb on + strand, within AMB_RS02525 at 522.795 kb on - strand, within AMB_RS02525 at 522.795 kb on - strand, within AMB_RS02525 at 522.795 kb on - strand, within AMB_RS02525 at 522.795 kb on - strand, within AMB_RS02525 at 522.892 kb on + strand, within AMB_RS02525 at 522.893 kb on - strand, within AMB_RS02525 at 523.085 kb on + strand at 523.085 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 520,255 + AMB_RS02510 0.57 +0.0 520,262 + AMB_RS02510 0.58 -1.0 520,263 - AMB_RS02510 0.58 +0.0 520,263 - AMB_RS02510 0.58 +0.4 520,263 - AMB_RS02510 0.58 -1.2 520,263 - AMB_RS02510 0.58 +0.6 520,289 + AMB_RS02510 0.60 -1.4 520,355 + AMB_RS02510 0.65 -1.6 520,356 - AMB_RS02510 0.65 +1.6 520,356 - AMB_RS02510 0.65 -1.0 520,457 + AMB_RS02510 0.74 -0.8 520,458 - AMB_RS02510 0.74 -1.2 520,458 - AMB_RS02510 0.74 -0.8 520,560 + AMB_RS02510 0.82 -0.2 520,561 - AMB_RS02510 0.82 -1.6 520,661 - -1.0 520,675 + -0.8 520,676 - +0.6 520,732 - +0.6 520,732 - -0.0 520,732 - +0.3 520,760 + +0.8 520,793 - -1.0 520,814 - -0.3 520,814 - -1.9 520,827 + +0.2 520,827 + -0.4 520,827 + +0.1 520,827 + +0.6 520,828 - +0.0 520,828 - -0.5 520,828 - -0.6 520,899 + -0.3 520,900 - -2.1 520,900 - -0.1 520,949 - -0.4 521,214 - +0.7 521,282 - AMB_RS02520 0.15 -0.4 521,357 + AMB_RS02520 0.22 -0.9 521,357 + AMB_RS02520 0.22 +0.2 521,358 - AMB_RS02520 0.22 -0.4 521,358 - AMB_RS02520 0.22 -3.1 521,429 + AMB_RS02520 0.29 -1.4 521,429 + AMB_RS02520 0.29 -0.5 521,429 + AMB_RS02520 0.29 -2.4 521,430 - AMB_RS02520 0.30 -1.8 521,510 + AMB_RS02520 0.37 -3.0 521,510 + AMB_RS02520 0.37 -2.2 521,510 + AMB_RS02520 0.37 +0.1 521,511 - AMB_RS02520 0.38 -2.2 521,511 - AMB_RS02520 0.38 -2.1 521,511 - AMB_RS02520 0.38 -0.2 521,583 + AMB_RS02520 0.45 -0.4 521,585 + AMB_RS02520 0.45 -2.7 521,586 - AMB_RS02520 0.45 -1.8 521,615 + AMB_RS02520 0.48 +0.0 521,615 + AMB_RS02520 0.48 -0.2 521,798 + AMB_RS02520 0.66 -0.0 521,798 + AMB_RS02520 0.66 -3.3 521,799 - AMB_RS02520 0.66 +1.6 521,799 - AMB_RS02520 0.66 -1.0 522,050 + -0.2 522,050 + +0.4 522,051 - -0.4 522,219 + -0.8 522,219 + -0.0 522,219 + -1.0 522,219 + -0.8 522,219 + -0.8 522,219 + +0.4 522,219 + +0.6 522,299 + -3.1 522,299 + -1.8 522,300 - -1.8 522,300 - -1.9 522,300 - -3.8 522,300 - -1.2 522,300 - -3.4 522,497 + AMB_RS02525 0.27 -2.7 522,497 + AMB_RS02525 0.27 -2.8 522,498 - AMB_RS02525 0.27 -0.0 522,498 - AMB_RS02525 0.27 -4.0 522,588 - AMB_RS02525 0.37 -2.6 522,674 - AMB_RS02525 0.46 -3.8 522,674 - AMB_RS02525 0.46 -4.6 522,674 - AMB_RS02525 0.46 -0.8 522,674 - AMB_RS02525 0.46 -3.8 522,674 - AMB_RS02525 0.46 -2.3 522,794 + AMB_RS02525 0.60 -3.9 522,794 + AMB_RS02525 0.60 -3.9 522,794 + AMB_RS02525 0.60 -0.8 522,794 + AMB_RS02525 0.60 -2.9 522,795 - AMB_RS02525 0.60 -4.0 522,795 - AMB_RS02525 0.60 -5.3 522,795 - AMB_RS02525 0.60 -3.2 522,795 - AMB_RS02525 0.60 -3.7 522,892 + AMB_RS02525 0.71 -3.8 522,893 - AMB_RS02525 0.71 -2.7 523,085 + -3.8 523,085 + -3.9
Or see this region's nucleotide sequence