Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01020 and AMB_RS01025 are separated by 163 nucleotides AMB_RS01025 and AMB_RS01030 are separated by 28 nucleotides AMB_RS01030 and AMB_RS01035 are separated by 232 nucleotides
AMB_RS01020: AMB_RS01020 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 226,191 to 227,393
_RS01020
AMB_RS01025: AMB_RS01025 - molecular chaperone GroEL, at 227,557 to 229,215
_RS01025
AMB_RS01030: AMB_RS01030 - co-chaperone GroES, at 229,244 to 229,531
_RS01030
AMB_RS01035: AMB_RS01035 - DUF2076 domain-containing protein, at 229,764 to 230,453
_RS01035
Position (kb) 227
228
229
230 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 227.435 kb on - strand at 227.558 kb on + strand at 227.558 kb on + strand at 227.558 kb on + strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 227.559 kb on - strand at 229.232 kb on - strand at 229.684 kb on - strand at 229.724 kb on + strand at 229.724 kb on + strand at 229.724 kb on + strand at 229.724 kb on + strand at 229.724 kb on + strand at 229.724 kb on + strand at 229.725 kb on - strand at 229.725 kb on - strand at 229.725 kb on - strand at 229.725 kb on - strand at 229.893 kb on + strand, within AMB_RS01035 at 229.893 kb on + strand, within AMB_RS01035 at 229.894 kb on - strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.097 kb on + strand, within AMB_RS01035 at 230.098 kb on - strand, within AMB_RS01035 at 230.098 kb on - strand, within AMB_RS01035 at 230.121 kb on + strand, within AMB_RS01035 at 230.122 kb on - strand, within AMB_RS01035 at 230.122 kb on - strand, within AMB_RS01035 at 230.122 kb on - strand, within AMB_RS01035 at 230.122 kb on - strand, within AMB_RS01035 at 230.122 kb on - strand, within AMB_RS01035
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 227,435 - -0.9 227,558 + +0.4 227,558 + -1.2 227,558 + -0.0 227,559 - -0.1 227,559 - +0.5 227,559 - -1.2 227,559 - -0.7 227,559 - -1.2 227,559 - -2.3 227,559 - -1.0 227,559 - +0.1 227,559 - -0.4 227,559 - -2.5 227,559 - -1.5 227,559 - -1.5 227,559 - -0.1 227,559 - -0.2 229,232 - -1.5 229,684 - +0.0 229,724 + -0.1 229,724 + +0.6 229,724 + -1.8 229,724 + +1.6 229,724 + +0.4 229,724 + -1.2 229,725 - -2.5 229,725 - -0.9 229,725 - +0.0 229,725 - +0.6 229,893 + AMB_RS01035 0.19 +0.1 229,893 + AMB_RS01035 0.19 -2.3 229,894 - AMB_RS01035 0.19 +0.4 230,097 + AMB_RS01035 0.48 +0.3 230,097 + AMB_RS01035 0.48 -1.5 230,097 + AMB_RS01035 0.48 -0.1 230,097 + AMB_RS01035 0.48 -0.9 230,097 + AMB_RS01035 0.48 +0.0 230,097 + AMB_RS01035 0.48 +0.2 230,097 + AMB_RS01035 0.48 +0.1 230,098 - AMB_RS01035 0.48 -0.3 230,098 - AMB_RS01035 0.48 +0.1 230,121 + AMB_RS01035 0.52 -1.9 230,122 - AMB_RS01035 0.52 +0.2 230,122 - AMB_RS01035 0.52 +0.5 230,122 - AMB_RS01035 0.52 -0.4 230,122 - AMB_RS01035 0.52 -3.2 230,122 - AMB_RS01035 0.52 -1.2
Or see this region's nucleotide sequence