Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00245 and AMB_RS00250 are separated by 69 nucleotides AMB_RS00250 and AMB_RS00255 are separated by 3 nucleotides AMB_RS00255 and AMB_RS00260 overlap by 23 nucleotides
AMB_RS00245: AMB_RS00245 - transcriptional activator RfaH, at 55,520 to 56,023
_RS00245
AMB_RS00250: AMB_RS00250 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 56,093 to 56,677
_RS00250
AMB_RS00255: AMB_RS00255 - methyltransferase, TIGR04325 family, at 56,681 to 57,454
_RS00255
AMB_RS00260: AMB_RS00260 - class I SAM-dependent methyltransferase, at 57,432 to 58,802
_RS00260
Position (kb) 56
57
58 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 55.732 kb on + strand, within AMB_RS00245 at 56.089 kb on + strand at 56.116 kb on - strand at 56.398 kb on + strand, within AMB_RS00250 at 56.482 kb on + strand, within AMB_RS00250 at 56.482 kb on + strand, within AMB_RS00250 at 56.513 kb on + strand, within AMB_RS00250 at 56.513 kb on + strand, within AMB_RS00250 at 56.513 kb on + strand, within AMB_RS00250 at 56.513 kb on + strand, within AMB_RS00250 at 56.513 kb on + strand, within AMB_RS00250 at 56.531 kb on + strand, within AMB_RS00250 at 56.531 kb on + strand, within AMB_RS00250 at 56.531 kb on + strand, within AMB_RS00250 at 56.531 kb on + strand, within AMB_RS00250 at 56.531 kb on + strand, within AMB_RS00250 at 56.531 kb on + strand, within AMB_RS00250 at 56.531 kb on + strand, within AMB_RS00250 at 56.695 kb on + strand at 56.695 kb on + strand at 56.711 kb on + strand at 56.711 kb on + strand at 56.711 kb on + strand at 56.711 kb on + strand at 56.712 kb on - strand at 56.843 kb on + strand, within AMB_RS00255 at 56.843 kb on + strand, within AMB_RS00255 at 56.843 kb on + strand, within AMB_RS00255 at 56.843 kb on + strand, within AMB_RS00255 at 56.844 kb on - strand, within AMB_RS00255 at 56.844 kb on - strand, within AMB_RS00255 at 56.873 kb on + strand, within AMB_RS00255 at 56.873 kb on + strand, within AMB_RS00255 at 56.873 kb on + strand, within AMB_RS00255 at 56.874 kb on - strand, within AMB_RS00255 at 56.874 kb on - strand, within AMB_RS00255 at 56.874 kb on - strand, within AMB_RS00255 at 56.930 kb on + strand, within AMB_RS00255 at 56.930 kb on + strand, within AMB_RS00255 at 56.930 kb on + strand, within AMB_RS00255 at 56.931 kb on - strand, within AMB_RS00255 at 56.931 kb on - strand, within AMB_RS00255 at 56.931 kb on - strand, within AMB_RS00255 at 56.984 kb on + strand, within AMB_RS00255 at 56.984 kb on + strand, within AMB_RS00255 at 56.984 kb on + strand, within AMB_RS00255 at 56.984 kb on + strand, within AMB_RS00255 at 56.984 kb on + strand, within AMB_RS00255 at 56.984 kb on + strand, within AMB_RS00255 at 56.984 kb on + strand, within AMB_RS00255 at 56.985 kb on - strand, within AMB_RS00255 at 56.985 kb on - strand, within AMB_RS00255 at 56.985 kb on - strand, within AMB_RS00255 at 56.985 kb on - strand, within AMB_RS00255 at 56.985 kb on - strand, within AMB_RS00255 at 56.986 kb on + strand, within AMB_RS00255 at 57.116 kb on + strand, within AMB_RS00255 at 57.116 kb on + strand, within AMB_RS00255 at 57.116 kb on + strand, within AMB_RS00255 at 57.117 kb on - strand, within AMB_RS00255 at 57.263 kb on + strand, within AMB_RS00255 at 57.264 kb on - strand, within AMB_RS00255 at 57.265 kb on + strand, within AMB_RS00255 at 57.265 kb on + strand, within AMB_RS00255 at 57.266 kb on - strand, within AMB_RS00255 at 57.266 kb on - strand, within AMB_RS00255 at 57.341 kb on + strand, within AMB_RS00255 at 57.530 kb on + strand at 57.530 kb on + strand at 57.530 kb on + strand at 57.530 kb on + strand at 57.531 kb on - strand at 57.533 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 55,732 + AMB_RS00245 0.42 +0.2 56,089 + +0.3 56,116 - -0.7 56,398 + AMB_RS00250 0.52 +0.1 56,482 + AMB_RS00250 0.66 -1.7 56,482 + AMB_RS00250 0.66 +0.2 56,513 + AMB_RS00250 0.72 -0.7 56,513 + AMB_RS00250 0.72 +0.1 56,513 + AMB_RS00250 0.72 -0.2 56,513 + AMB_RS00250 0.72 +0.1 56,513 + AMB_RS00250 0.72 +0.1 56,531 + AMB_RS00250 0.75 -1.4 56,531 + AMB_RS00250 0.75 -1.8 56,531 + AMB_RS00250 0.75 -0.9 56,531 + AMB_RS00250 0.75 -0.1 56,531 + AMB_RS00250 0.75 -0.4 56,531 + AMB_RS00250 0.75 -1.4 56,531 + AMB_RS00250 0.75 -0.4 56,695 + -0.1 56,695 + -0.3 56,711 + -1.9 56,711 + -0.1 56,711 + -0.4 56,711 + -0.4 56,712 - -0.7 56,843 + AMB_RS00255 0.21 +1.6 56,843 + AMB_RS00255 0.21 -0.4 56,843 + AMB_RS00255 0.21 +1.0 56,843 + AMB_RS00255 0.21 +0.0 56,844 - AMB_RS00255 0.21 -0.7 56,844 - AMB_RS00255 0.21 -0.7 56,873 + AMB_RS00255 0.25 +0.0 56,873 + AMB_RS00255 0.25 +0.1 56,873 + AMB_RS00255 0.25 +0.1 56,874 - AMB_RS00255 0.25 -1.5 56,874 - AMB_RS00255 0.25 +0.1 56,874 - AMB_RS00255 0.25 +0.1 56,930 + AMB_RS00255 0.32 +0.4 56,930 + AMB_RS00255 0.32 -0.2 56,930 + AMB_RS00255 0.32 +0.2 56,931 - AMB_RS00255 0.32 -2.8 56,931 - AMB_RS00255 0.32 -1.5 56,931 - AMB_RS00255 0.32 -2.4 56,984 + AMB_RS00255 0.39 -0.4 56,984 + AMB_RS00255 0.39 -1.2 56,984 + AMB_RS00255 0.39 +0.5 56,984 + AMB_RS00255 0.39 +0.1 56,984 + AMB_RS00255 0.39 +0.2 56,984 + AMB_RS00255 0.39 -0.0 56,984 + AMB_RS00255 0.39 +0.1 56,985 - AMB_RS00255 0.39 -3.8 56,985 - AMB_RS00255 0.39 +0.1 56,985 - AMB_RS00255 0.39 -1.4 56,985 - AMB_RS00255 0.39 -0.4 56,985 - AMB_RS00255 0.39 -1.4 56,986 + AMB_RS00255 0.39 -0.3 57,116 + AMB_RS00255 0.56 -0.9 57,116 + AMB_RS00255 0.56 +0.0 57,116 + AMB_RS00255 0.56 +0.4 57,117 - AMB_RS00255 0.56 -1.8 57,263 + AMB_RS00255 0.75 +0.3 57,264 - AMB_RS00255 0.75 +0.6 57,265 + AMB_RS00255 0.75 -0.3 57,265 + AMB_RS00255 0.75 +0.1 57,266 - AMB_RS00255 0.76 +1.6 57,266 - AMB_RS00255 0.76 -2.8 57,341 + AMB_RS00255 0.85 -1.5 57,530 + -0.3 57,530 + +0.1 57,530 + +0.6 57,530 + -0.3 57,531 - -0.4 57,533 + -0.4
Or see this region's nucleotide sequence