Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00120 and AMB_RS00125 are separated by 29 nucleotides AMB_RS00125 and AMB_RS00130 are separated by 7 nucleotides AMB_RS00130 and AMB_RS00135 are separated by 67 nucleotides
AMB_RS00120: AMB_RS00120 - YggS family pyridoxal phosphate-dependent enzyme, at 22,612 to 23,262
_RS00120
AMB_RS00125: AMB_RS00125 - thiamine phosphate synthase, at 23,292 to 23,891
_RS00125
AMB_RS00130: AMB_RS00130 - tetratricopeptide repeat protein, at 23,899 to 25,428
_RS00130
AMB_RS00135: AMB_RS00135 - porin, at 25,496 to 26,845
_RS00135
Position (kb) 23
24
25
26 Strain fitness (log2 ratio) -1
0
1 at 23.240 kb on - strand at 23.240 kb on - strand at 23.281 kb on - strand at 23.890 kb on - strand at 23.987 kb on + strand at 24.032 kb on + strand at 24.411 kb on - strand, within AMB_RS00130 at 24.460 kb on + strand, within AMB_RS00130 at 24.480 kb on + strand, within AMB_RS00130 at 24.481 kb on - strand, within AMB_RS00130 at 24.519 kb on - strand, within AMB_RS00130 at 24.643 kb on - strand, within AMB_RS00130 at 24.755 kb on + strand, within AMB_RS00130 at 24.875 kb on + strand, within AMB_RS00130 at 24.875 kb on + strand, within AMB_RS00130 at 24.941 kb on - strand, within AMB_RS00130 at 25.428 kb on + strand at 25.429 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 23,240 - +0.0 23,240 - -0.7 23,281 - +0.6 23,890 - -0.7 23,987 + +0.0 24,032 + +1.6 24,411 - AMB_RS00130 0.33 +1.6 24,460 + AMB_RS00130 0.37 +0.9 24,480 + AMB_RS00130 0.38 -0.7 24,481 - AMB_RS00130 0.38 -0.1 24,519 - AMB_RS00130 0.41 -1.2 24,643 - AMB_RS00130 0.49 -0.1 24,755 + AMB_RS00130 0.56 -0.4 24,875 + AMB_RS00130 0.64 +0.1 24,875 + AMB_RS00130 0.64 +1.6 24,941 - AMB_RS00130 0.68 -0.6 25,428 + -0.7 25,429 - -0.9
Or see this region's nucleotide sequence