Experiment: 30_an_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00005 and AMB_RS00010 are separated by 15 nucleotides AMB_RS00010 and AMB_RS00015 are separated by 39 nucleotides AMB_RS00015 and AMB_RS00020 overlap by 20 nucleotides
AMB_RS00005: AMB_RS00005 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE, at 11 to 1,336
_RS00005
AMB_RS00010: AMB_RS00010 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG, at 1,352 to 3,265
_RS00010
AMB_RS00015: AMB_RS00015 - 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG, at 3,305 to 3,937
_RS00015
AMB_RS00020: AMB_RS00020 - ParA family protein, at 3,918 to 4,715
_RS00020
Position (kb) 1
2
3
4 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1.364 kb on - strand at 2.171 kb on + strand, within AMB_RS00010 at 2.604 kb on - strand, within AMB_RS00010 at 2.604 kb on - strand, within AMB_RS00010 at 3.009 kb on - strand, within AMB_RS00010 at 3.386 kb on + strand, within AMB_RS00015 at 3.453 kb on + strand, within AMB_RS00015 at 3.453 kb on + strand, within AMB_RS00015 at 3.453 kb on + strand, within AMB_RS00015 at 3.847 kb on + strand, within AMB_RS00015 at 3.847 kb on + strand, within AMB_RS00015
Per-strain Table
Position Strand Gene LocusTag Fraction 30_an_initial_1 remove 1,364 - +0.1 2,171 + AMB_RS00010 0.43 -1.5 2,604 - AMB_RS00010 0.65 +0.5 2,604 - AMB_RS00010 0.65 -2.4 3,009 - AMB_RS00010 0.87 -2.8 3,386 + AMB_RS00015 0.13 +0.1 3,453 + AMB_RS00015 0.23 -2.5 3,453 + AMB_RS00015 0.23 -1.7 3,453 + AMB_RS00015 0.23 -0.0 3,847 + AMB_RS00015 0.86 -1.5 3,847 + AMB_RS00015 0.86 -0.6
Or see this region's nucleotide sequence