Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt napA and napD overlap by 4 nucleotides napD and napF overlap by 11 nucleotides napF and yojO are separated by 108 nucleotides yojO and eco are separated by 134 nucleotides
b2206: napA - nitrate reductase, periplasmic, large subunit (NCBI), at 2,298,289 to 2,300,775
napA
b2207: napD - assembly protein for periplasmic nitrate reductase (NCBI), at 2,300,772 to 2,301,035
napD
b2208: napF - ferredoxin-type protein, predicted role in electron transfer to periplasmic nitrate reductase (NapA) (NCBI), at 2,301,025 to 2,301,519
napF
b4604: yojO - hypothetical protein (RefSeq), at 2,301,628 to 2,301,792
yojO
b2209: eco - ecotin precursor (NCBI), at 2,301,927 to 2,302,415
eco
Position (kb)
2301
2302 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2300.832 kb on + strand, within napD at 2300.841 kb on + strand, within napD at 2300.865 kb on - strand, within napD at 2300.965 kb on + strand, within napD at 2301.037 kb on + strand at 2301.037 kb on + strand at 2301.060 kb on + strand at 2301.060 kb on + strand at 2301.084 kb on + strand, within napF at 2301.183 kb on - strand, within napF at 2301.210 kb on - strand, within napF at 2301.210 kb on - strand, within napF at 2301.256 kb on - strand, within napF at 2301.257 kb on - strand, within napF at 2301.286 kb on + strand, within napF at 2301.415 kb on - strand, within napF at 2301.496 kb on - strand at 2301.620 kb on + strand at 2301.874 kb on + strand at 2302.083 kb on + strand, within eco at 2302.294 kb on + strand, within eco at 2302.310 kb on - strand, within eco at 2302.522 kb on + strand at 2302.748 kb on + strand at 2302.763 kb on + strand at 2302.763 kb on + strand at 2302.773 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 2,300,832 + napD b2207 0.23 +0.7 2,300,841 + napD b2207 0.26 -1.2 2,300,865 - napD b2207 0.35 -0.1 2,300,965 + napD b2207 0.73 +1.7 2,301,037 + -1.2 2,301,037 + -1.5 2,301,060 + -1.9 2,301,060 + +1.9 2,301,084 + napF b2208 0.12 +1.5 2,301,183 - napF b2208 0.32 -0.2 2,301,210 - napF b2208 0.37 -0.5 2,301,210 - napF b2208 0.37 +1.7 2,301,256 - napF b2208 0.47 -4.2 2,301,257 - napF b2208 0.47 -0.3 2,301,286 + napF b2208 0.53 -2.2 2,301,415 - napF b2208 0.79 +0.2 2,301,496 - +1.4 2,301,620 + -0.9 2,301,874 + -0.9 2,302,083 + eco b2209 0.32 +0.0 2,302,294 + eco b2209 0.75 -0.5 2,302,310 - eco b2209 0.78 -0.8 2,302,522 + +0.6 2,302,748 + +0.7 2,302,763 + +0.5 2,302,763 + +0.5 2,302,773 + -1.2
Or see this region's nucleotide sequence