Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt coaA and thrU are separated by 361 nucleotides thrU and tyrU are separated by 8 nucleotides tyrU and glyT are separated by 116 nucleotides glyT and thrT are separated by 6 nucleotides thrT and tufB are separated by 114 nucleotides
b3974: coaA - pantothenate kinase (NCBI), at 4,172,099 to 4,173,049
coaA
b3976: thrU - tRNA-Thr (NCBI), at 4,173,411 to 4,173,486
thrU
b3977: tyrU - tRNA-Tyr (NCBI), at 4,173,495 to 4,173,579
tyrU
b3978: glyT - tRNA-Gly (NCBI), at 4,173,696 to 4,173,770
glyT
b3979: thrT - tRNA-Thr (NCBI), at 4,173,777 to 4,173,852
thrT
b3980: tufB - protein chain elongation factor EF-Tu (duplicate of tufA) (NCBI), at 4,173,967 to 4,175,151
tufB
Position (kb)
4173
4174 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 4172.981 kb on - strand at 4172.981 kb on - strand at 4173.047 kb on - strand at 4173.074 kb on - strand at 4173.125 kb on + strand at 4173.139 kb on - strand at 4173.139 kb on - strand at 4173.147 kb on - strand at 4173.155 kb on - strand at 4173.219 kb on - strand at 4173.308 kb on - strand at 4173.399 kb on + strand at 4173.546 kb on + strand, within tyrU at 4173.781 kb on + strand at 4173.813 kb on - strand, within thrT at 4173.861 kb on - strand at 4173.861 kb on - strand at 4173.912 kb on - strand at 4173.916 kb on + strand at 4173.929 kb on + strand at 4173.989 kb on + strand at 4174.016 kb on - strand at 4174.082 kb on - strand at 4174.082 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 4,172,981 - -1.2 4,172,981 - +0.6 4,173,047 - +0.2 4,173,074 - -1.5 4,173,125 + +1.0 4,173,139 - -0.4 4,173,139 - +0.2 4,173,147 - -0.0 4,173,155 - +0.0 4,173,219 - +0.9 4,173,308 - +0.7 4,173,399 + +5.7 4,173,546 + tyrU b3977 0.60 +3.6 4,173,781 + +4.6 4,173,813 - thrT b3979 0.47 +4.7 4,173,861 - +5.0 4,173,861 - -0.6 4,173,912 - +3.7 4,173,916 + +2.4 4,173,929 + +2.3 4,173,989 + +3.4 4,174,016 - +3.5 4,174,082 - +5.7 4,174,082 - +1.2
Or see this region's nucleotide sequence