Strain Fitness in Escherichia coli BW25113 around b3977

Experiment: Benzalkonium Chloride 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcoaA and thrU are separated by 361 nucleotidesthrU and tyrU are separated by 8 nucleotidestyrU and glyT are separated by 116 nucleotidesglyT and thrT are separated by 6 nucleotidesthrT and tufB are separated by 114 nucleotides b3974: coaA - pantothenate kinase (NCBI), at 4,172,099 to 4,173,049 coaA b3976: thrU - tRNA-Thr (NCBI), at 4,173,411 to 4,173,486 thrU b3977: tyrU - tRNA-Tyr (NCBI), at 4,173,495 to 4,173,579 tyrU b3978: glyT - tRNA-Gly (NCBI), at 4,173,696 to 4,173,770 glyT b3979: thrT - tRNA-Thr (NCBI), at 4,173,777 to 4,173,852 thrT b3980: tufB - protein chain elongation factor EF-Tu (duplicate of tufA) (NCBI), at 4,173,967 to 4,175,151 tufB Position (kb) 4173 4174Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 4172.981 kb on - strandat 4172.981 kb on - strandat 4173.047 kb on - strandat 4173.074 kb on - strandat 4173.125 kb on + strandat 4173.139 kb on - strandat 4173.139 kb on - strandat 4173.147 kb on - strandat 4173.155 kb on - strandat 4173.219 kb on - strandat 4173.308 kb on - strandat 4173.399 kb on + strandat 4173.546 kb on + strand, within tyrUat 4173.781 kb on + strandat 4173.813 kb on - strand, within thrTat 4173.861 kb on - strandat 4173.861 kb on - strandat 4173.912 kb on - strandat 4173.916 kb on + strandat 4173.929 kb on + strandat 4173.989 kb on + strandat 4174.016 kb on - strandat 4174.082 kb on - strandat 4174.082 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml
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4,172,981 - -1.2
4,172,981 - +0.6
4,173,047 - +0.2
4,173,074 - -1.5
4,173,125 + +1.0
4,173,139 - -0.4
4,173,139 - +0.2
4,173,147 - -0.0
4,173,155 - +0.0
4,173,219 - +0.9
4,173,308 - +0.7
4,173,399 + +5.7
4,173,546 + tyrU b3977 0.60 +3.6
4,173,781 + +4.6
4,173,813 - thrT b3979 0.47 +4.7
4,173,861 - +5.0
4,173,861 - -0.6
4,173,912 - +3.7
4,173,916 + +2.4
4,173,929 + +2.3
4,173,989 + +3.4
4,174,016 - +3.5
4,174,082 - +5.7
4,174,082 - +1.2

Or see this region's nucleotide sequence