Strain Fitness in Escherichia coli BW25113 around b2715

Experiment: Benzalkonium Chloride 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntascG and ascF are separated by 259 nucleotidesascF and ascB are separated by 8 nucleotides b2714: ascG - DNA-binding transcriptional repressor (NCBI), at 2,836,276 to 2,837,286 ascG b2715: ascF - fused cellobiose/arbutin/salicin-specific PTS enzymes: IIB component/IC component (NCBI), at 2,837,546 to 2,839,003 ascF b2716: ascB - cryptic 6-phospho-beta-glucosidase (NCBI), at 2,839,012 to 2,840,436 ascB Position (kb) 2837 2838 2839 2840Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2836.653 kb on + strand, within ascGat 2836.653 kb on + strand, within ascGat 2836.653 kb on + strand, within ascGat 2836.653 kb on + strand, within ascGat 2836.687 kb on - strand, within ascGat 2836.754 kb on - strand, within ascGat 2836.760 kb on + strand, within ascGat 2836.768 kb on - strand, within ascGat 2836.768 kb on - strand, within ascGat 2836.802 kb on - strand, within ascGat 2836.802 kb on - strand, within ascGat 2836.810 kb on + strand, within ascGat 2836.877 kb on - strand, within ascGat 2837.055 kb on - strandat 2837.099 kb on - strand, within ascGat 2837.099 kb on - strand, within ascGat 2837.171 kb on - strand, within ascGat 2837.318 kb on + strandat 2837.318 kb on + strandat 2837.323 kb on - strandat 2837.323 kb on - strandat 2837.349 kb on - strandat 2837.518 kb on - strandat 2837.583 kb on - strandat 2837.591 kb on - strandat 2837.591 kb on - strandat 2837.626 kb on + strandat 2837.626 kb on + strandat 2837.626 kb on + strandat 2837.631 kb on - strandat 2837.634 kb on - strandat 2837.666 kb on + strandat 2837.717 kb on - strand, within ascFat 2837.766 kb on - strand, within ascFat 2837.766 kb on - strand, within ascFat 2837.804 kb on - strand, within ascFat 2838.003 kb on - strand, within ascFat 2838.101 kb on + strand, within ascFat 2838.101 kb on + strand, within ascFat 2838.120 kb on + strand, within ascFat 2838.188 kb on + strand, within ascFat 2838.188 kb on + strand, within ascFat 2838.238 kb on + strand, within ascFat 2838.238 kb on + strand, within ascFat 2838.264 kb on + strand, within ascFat 2838.272 kb on - strand, within ascFat 2838.336 kb on - strand, within ascFat 2838.388 kb on + strand, within ascFat 2838.388 kb on + strand, within ascFat 2838.396 kb on - strand, within ascFat 2838.398 kb on + strand, within ascFat 2838.533 kb on + strand, within ascFat 2838.533 kb on + strand, within ascFat 2838.676 kb on + strand, within ascFat 2838.676 kb on + strand, within ascFat 2838.786 kb on - strand, within ascFat 2838.786 kb on - strand, within ascFat 2838.879 kb on - strandat 2839.120 kb on - strandat 2839.120 kb on - strandat 2839.234 kb on - strand, within ascBat 2839.234 kb on - strand, within ascBat 2839.385 kb on + strand, within ascBat 2839.387 kb on + strand, within ascBat 2839.407 kb on + strand, within ascBat 2839.407 kb on + strand, within ascBat 2839.427 kb on + strand, within ascBat 2839.514 kb on + strand, within ascBat 2839.520 kb on + strand, within ascBat 2839.556 kb on + strand, within ascBat 2839.607 kb on + strand, within ascBat 2839.661 kb on - strand, within ascBat 2839.893 kb on - strand, within ascBat 2839.893 kb on - strand, within ascB

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml
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2,836,653 + ascG b2714 0.37 +1.1
2,836,653 + ascG b2714 0.37 +1.2
2,836,653 + ascG b2714 0.37 +0.2
2,836,653 + ascG b2714 0.37 -1.9
2,836,687 - ascG b2714 0.41 -1.0
2,836,754 - ascG b2714 0.47 -0.3
2,836,760 + ascG b2714 0.48 -0.4
2,836,768 - ascG b2714 0.49 +0.7
2,836,768 - ascG b2714 0.49 +0.2
2,836,802 - ascG b2714 0.52 +0.4
2,836,802 - ascG b2714 0.52 -0.8
2,836,810 + ascG b2714 0.53 +1.4
2,836,877 - ascG b2714 0.59 +0.2
2,837,055 - +0.4
2,837,099 - ascG b2714 0.81 +2.0
2,837,099 - ascG b2714 0.81 +0.1
2,837,171 - ascG b2714 0.89 -0.0
2,837,318 + +0.1
2,837,318 + -1.4
2,837,323 - +1.1
2,837,323 - -0.2
2,837,349 - +0.3
2,837,518 - -0.4
2,837,583 - +2.1
2,837,591 - -1.2
2,837,591 - +2.5
2,837,626 + -1.4
2,837,626 + -0.9
2,837,626 + +0.4
2,837,631 - -2.9
2,837,634 - +1.4
2,837,666 + +1.0
2,837,717 - ascF b2715 0.12 -1.9
2,837,766 - ascF b2715 0.15 -0.6
2,837,766 - ascF b2715 0.15 -0.9
2,837,804 - ascF b2715 0.18 +2.1
2,838,003 - ascF b2715 0.31 -0.9
2,838,101 + ascF b2715 0.38 +0.3
2,838,101 + ascF b2715 0.38 -0.6
2,838,120 + ascF b2715 0.39 +0.0
2,838,188 + ascF b2715 0.44 -1.4
2,838,188 + ascF b2715 0.44 -1.9
2,838,238 + ascF b2715 0.47 +1.4
2,838,238 + ascF b2715 0.47 -0.9
2,838,264 + ascF b2715 0.49 +1.4
2,838,272 - ascF b2715 0.50 +0.8
2,838,336 - ascF b2715 0.54 -1.0
2,838,388 + ascF b2715 0.58 -1.6
2,838,388 + ascF b2715 0.58 +0.5
2,838,396 - ascF b2715 0.58 -1.1
2,838,398 + ascF b2715 0.58 +1.4
2,838,533 + ascF b2715 0.68 -1.2
2,838,533 + ascF b2715 0.68 -0.1
2,838,676 + ascF b2715 0.78 +0.1
2,838,676 + ascF b2715 0.78 +1.4
2,838,786 - ascF b2715 0.85 -0.9
2,838,786 - ascF b2715 0.85 +1.4
2,838,879 - -0.6
2,839,120 - -1.4
2,839,120 - +2.8
2,839,234 - ascB b2716 0.16 -1.4
2,839,234 - ascB b2716 0.16 +0.8
2,839,385 + ascB b2716 0.26 -0.4
2,839,387 + ascB b2716 0.26 -2.5
2,839,407 + ascB b2716 0.28 -1.2
2,839,407 + ascB b2716 0.28 -0.9
2,839,427 + ascB b2716 0.29 +0.6
2,839,514 + ascB b2716 0.35 -0.2
2,839,520 + ascB b2716 0.36 +0.8
2,839,556 + ascB b2716 0.38 +0.7
2,839,607 + ascB b2716 0.42 +0.6
2,839,661 - ascB b2716 0.46 +1.3
2,839,893 - ascB b2716 0.62 +1.2
2,839,893 - ascB b2716 0.62 +0.6

Or see this region's nucleotide sequence