Strain Fitness in Escherichia coli BW25113 around b4074

Experiment: Benzalkonium Chloride 0.004 mg/ml

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntnrfD and nrfE are separated by 79 nucleotidesnrfE and nrfF overlap by 8 nucleotidesnrfF and nrfG overlap by 4 nucleotides b4073: nrfD - formate-dependent nitrite reductase, membrane subunit (NCBI), at 4,288,499 to 4,289,455 nrfD b4074: nrfE - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfE (NCBI), at 4,289,535 to 4,291,193 nrfE b4075: nrfF - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfF (NCBI), at 4,291,186 to 4,291,569 nrfF b4076: nrfG - heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG (NCBI), at 4,291,566 to 4,292,162 nrfG Position (kb) 4289 4290 4291 4292Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4288.543 kb on - strandat 4288.617 kb on - strand, within nrfDat 4288.617 kb on - strand, within nrfDat 4288.650 kb on - strand, within nrfDat 4288.650 kb on - strand, within nrfDat 4288.655 kb on - strand, within nrfDat 4288.732 kb on + strand, within nrfDat 4288.740 kb on + strand, within nrfDat 4288.804 kb on + strand, within nrfDat 4288.804 kb on + strand, within nrfDat 4288.807 kb on + strand, within nrfDat 4288.825 kb on - strand, within nrfDat 4288.825 kb on - strand, within nrfDat 4288.888 kb on + strand, within nrfDat 4288.972 kb on + strand, within nrfDat 4288.972 kb on + strand, within nrfDat 4288.978 kb on + strand, within nrfDat 4288.978 kb on + strand, within nrfDat 4289.100 kb on - strand, within nrfDat 4289.154 kb on + strand, within nrfDat 4289.154 kb on + strandat 4289.163 kb on + strand, within nrfDat 4289.170 kb on + strand, within nrfDat 4289.170 kb on + strand, within nrfDat 4289.324 kb on + strand, within nrfDat 4289.324 kb on + strand, within nrfDat 4289.324 kb on + strand, within nrfDat 4289.361 kb on + strandat 4289.380 kb on + strandat 4289.543 kb on - strandat 4289.576 kb on - strandat 4289.621 kb on + strandat 4289.621 kb on + strandat 4289.647 kb on + strandat 4289.707 kb on + strand, within nrfEat 4289.707 kb on + strand, within nrfEat 4289.786 kb on + strand, within nrfEat 4289.896 kb on + strand, within nrfEat 4289.914 kb on - strand, within nrfEat 4289.914 kb on - strand, within nrfEat 4289.932 kb on + strand, within nrfEat 4289.932 kb on + strandat 4289.985 kb on + strand, within nrfEat 4289.985 kb on + strand, within nrfEat 4290.140 kb on + strand, within nrfEat 4290.255 kb on + strand, within nrfEat 4290.255 kb on + strand, within nrfEat 4290.265 kb on - strand, within nrfEat 4290.415 kb on - strand, within nrfEat 4290.415 kb on - strand, within nrfEat 4290.441 kb on - strand, within nrfEat 4290.443 kb on + strand, within nrfEat 4290.443 kb on + strand, within nrfEat 4290.465 kb on + strand, within nrfEat 4290.465 kb on - strand, within nrfEat 4290.465 kb on - strand, within nrfEat 4290.566 kb on + strand, within nrfEat 4290.572 kb on - strand, within nrfEat 4290.619 kb on + strand, within nrfEat 4290.619 kb on + strand, within nrfEat 4290.726 kb on + strand, within nrfEat 4290.797 kb on - strand, within nrfEat 4290.832 kb on + strand, within nrfEat 4290.839 kb on + strand, within nrfEat 4290.839 kb on + strand, within nrfEat 4290.847 kb on - strand, within nrfEat 4290.930 kb on + strand, within nrfEat 4290.952 kb on + strand, within nrfEat 4291.075 kb on + strandat 4291.075 kb on + strandat 4291.155 kb on + strandat 4291.208 kb on - strandat 4291.236 kb on + strand, within nrfFat 4291.270 kb on + strand, within nrfFat 4291.423 kb on - strand, within nrfFat 4291.464 kb on + strand, within nrfFat 4291.472 kb on - strand, within nrfFat 4291.483 kb on + strand, within nrfFat 4291.497 kb on + strand, within nrfFat 4291.503 kb on + strand, within nrfFat 4291.503 kb on + strand, within nrfFat 4291.711 kb on - strand, within nrfGat 4291.800 kb on - strand, within nrfGat 4291.854 kb on + strand, within nrfGat 4291.859 kb on + strand, within nrfGat 4291.911 kb on + strand, within nrfGat 4291.911 kb on + strand, within nrfGat 4291.934 kb on + strand, within nrfGat 4291.982 kb on + strand, within nrfGat 4291.982 kb on + strand, within nrfGat 4292.106 kb on + strandat 4292.109 kb on + strandat 4292.112 kb on - strandat 4292.112 kb on - strandat 4292.114 kb on + strandat 4292.114 kb on + strandat 4292.131 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml
remove
4,288,543 - +1.0
4,288,617 - nrfD b4073 0.12 -1.7
4,288,617 - nrfD b4073 0.12 +1.8
4,288,650 - nrfD b4073 0.16 -0.3
4,288,650 - nrfD b4073 0.16 -0.4
4,288,655 - nrfD b4073 0.16 +1.5
4,288,732 + nrfD b4073 0.24 -1.5
4,288,740 + nrfD b4073 0.25 +2.2
4,288,804 + nrfD b4073 0.32 +1.6
4,288,804 + nrfD b4073 0.32 +1.3
4,288,807 + nrfD b4073 0.32 -2.8
4,288,825 - nrfD b4073 0.34 +2.8
4,288,825 - nrfD b4073 0.34 +0.5
4,288,888 + nrfD b4073 0.41 +2.4
4,288,972 + nrfD b4073 0.49 +1.6
4,288,972 + nrfD b4073 0.49 +0.9
4,288,978 + nrfD b4073 0.50 -2.4
4,288,978 + nrfD b4073 0.50 -0.1
4,289,100 - nrfD b4073 0.63 +1.2
4,289,154 + nrfD b4073 0.68 +2.1
4,289,154 + +1.6
4,289,163 + nrfD b4073 0.69 +1.3
4,289,170 + nrfD b4073 0.70 +0.1
4,289,170 + nrfD b4073 0.70 +1.2
4,289,324 + nrfD b4073 0.86 +1.2
4,289,324 + nrfD b4073 0.86 +0.1
4,289,324 + nrfD b4073 0.86 +1.1
4,289,361 + -0.4
4,289,380 + -1.2
4,289,543 - -0.7
4,289,576 - +1.3
4,289,621 + -0.4
4,289,621 + +1.1
4,289,647 + +1.8
4,289,707 + nrfE b4074 0.10 -0.4
4,289,707 + nrfE b4074 0.10 -0.4
4,289,786 + nrfE b4074 0.15 -1.0
4,289,896 + nrfE b4074 0.22 +1.6
4,289,914 - nrfE b4074 0.23 +0.3
4,289,914 - nrfE b4074 0.23 -1.0
4,289,932 + nrfE b4074 0.24 -0.7
4,289,932 + +0.6
4,289,985 + nrfE b4074 0.27 +1.6
4,289,985 + nrfE b4074 0.27 +0.9
4,290,140 + nrfE b4074 0.36 +2.2
4,290,255 + nrfE b4074 0.43 -1.0
4,290,255 + nrfE b4074 0.43 -0.1
4,290,265 - nrfE b4074 0.44 -0.8
4,290,415 - nrfE b4074 0.53 +0.8
4,290,415 - nrfE b4074 0.53 +1.0
4,290,441 - nrfE b4074 0.55 -2.3
4,290,443 + nrfE b4074 0.55 -2.5
4,290,443 + nrfE b4074 0.55 -1.1
4,290,465 + nrfE b4074 0.56 +0.8
4,290,465 - nrfE b4074 0.56 -0.4
4,290,465 - nrfE b4074 0.56 +0.6
4,290,566 + nrfE b4074 0.62 +0.6
4,290,572 - nrfE b4074 0.63 -1.0
4,290,619 + nrfE b4074 0.65 -1.8
4,290,619 + nrfE b4074 0.65 -1.8
4,290,726 + nrfE b4074 0.72 -2.4
4,290,797 - nrfE b4074 0.76 -0.4
4,290,832 + nrfE b4074 0.78 -0.9
4,290,839 + nrfE b4074 0.79 +2.2
4,290,839 + nrfE b4074 0.79 +1.6
4,290,847 - nrfE b4074 0.79 +0.2
4,290,930 + nrfE b4074 0.84 -2.2
4,290,952 + nrfE b4074 0.85 -1.4
4,291,075 + +0.6
4,291,075 + +1.6
4,291,155 + -2.0
4,291,208 - +1.6
4,291,236 + nrfF b4075 0.13 -2.8
4,291,270 + nrfF b4075 0.22 -1.2
4,291,423 - nrfF b4075 0.62 +1.4
4,291,464 + nrfF b4075 0.72 +0.2
4,291,472 - nrfF b4075 0.74 -0.8
4,291,483 + nrfF b4075 0.77 +0.3
4,291,497 + nrfF b4075 0.81 +0.0
4,291,503 + nrfF b4075 0.83 +1.6
4,291,503 + nrfF b4075 0.83 -1.3
4,291,711 - nrfG b4076 0.24 -1.7
4,291,800 - nrfG b4076 0.39 -1.1
4,291,854 + nrfG b4076 0.48 -2.8
4,291,859 + nrfG b4076 0.49 -0.8
4,291,911 + nrfG b4076 0.58 -2.4
4,291,911 + nrfG b4076 0.58 -1.2
4,291,934 + nrfG b4076 0.62 -0.9
4,291,982 + nrfG b4076 0.70 -2.4
4,291,982 + nrfG b4076 0.70 -0.2
4,292,106 + +0.0
4,292,109 + +0.0
4,292,112 - -1.4
4,292,112 - +1.5
4,292,114 + -1.0
4,292,114 + -0.4
4,292,131 - +0.6

Or see this region's nucleotide sequence