Strain Fitness in Escherichia coli BW25113 around b4022

Experiment: Benzalkonium Chloride 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyjbB and pepE are separated by 90 nucleotidespepE and yjbC are separated by 211 nucleotidesyjbC and yjbD are separated by 132 nucleotidesyjbD and lysC are separated by 252 nucleotides b4020: yjbB - predicted transporter (NCBI), at 4,225,754 to 4,227,385 yjbB b4021: pepE - peptidase E (NCBI), at 4,227,476 to 4,228,165 pepE b4022: yjbC - 23S rRNA pseudouridine synthase (NCBI), at 4,228,377 to 4,229,249 yjbC b4023: yjbD - hypothetical protein (NCBI), at 4,229,382 to 4,229,654 yjbD b4024: lysC - aspartate kinase III (NCBI), at 4,229,907 to 4,231,256 lysC Position (kb) 4228 4229 4230Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 4227.407 kb on + strandat 4227.427 kb on + strandat 4227.462 kb on - strandat 4227.509 kb on + strandat 4227.509 kb on + strandat 4227.527 kb on - strandat 4227.527 kb on - strandat 4227.534 kb on - strandat 4227.551 kb on - strand, within pepEat 4227.556 kb on - strand, within pepEat 4227.556 kb on - strand, within pepEat 4227.621 kb on - strand, within pepEat 4227.626 kb on + strand, within pepEat 4227.626 kb on + strand, within pepEat 4227.634 kb on + strand, within pepEat 4227.634 kb on + strand, within pepEat 4227.657 kb on - strand, within pepEat 4227.739 kb on + strand, within pepEat 4227.739 kb on + strand, within pepEat 4227.772 kb on + strand, within pepEat 4227.796 kb on - strand, within pepEat 4227.796 kb on - strand, within pepEat 4227.813 kb on + strand, within pepEat 4227.821 kb on - strand, within pepEat 4227.887 kb on - strand, within pepEat 4227.887 kb on - strand, within pepEat 4227.888 kb on - strand, within pepEat 4227.888 kb on - strand, within pepEat 4227.993 kb on + strand, within pepEat 4228.039 kb on - strand, within pepEat 4228.077 kb on + strand, within pepEat 4228.087 kb on - strand, within pepEat 4228.087 kb on - strand, within pepEat 4228.118 kb on - strandat 4228.118 kb on - strandat 4228.119 kb on - strandat 4228.125 kb on - strandat 4228.125 kb on - strandat 4228.159 kb on + strandat 4228.162 kb on + strandat 4228.162 kb on + strandat 4228.200 kb on + strandat 4228.200 kb on + strandat 4228.223 kb on + strandat 4228.223 kb on + strandat 4228.225 kb on + strandat 4228.225 kb on + strandat 4228.225 kb on + strandat 4228.231 kb on - strandat 4228.231 kb on - strandat 4228.231 kb on - strandat 4228.235 kb on + strandat 4228.235 kb on + strandat 4228.235 kb on + strandat 4228.252 kb on - strandat 4228.267 kb on + strandat 4228.303 kb on + strandat 4228.344 kb on - strandat 4228.344 kb on - strandat 4228.369 kb on - strandat 4228.369 kb on - strandat 4228.387 kb on + strandat 4228.451 kb on + strandat 4228.451 kb on + strandat 4228.451 kb on + strandat 4228.451 kb on + strandat 4228.459 kb on - strandat 4228.459 kb on - strandat 4228.459 kb on - strandat 4228.461 kb on + strandat 4228.478 kb on + strand, within yjbCat 4228.478 kb on - strand, within yjbCat 4228.488 kb on - strand, within yjbCat 4228.498 kb on + strand, within yjbCat 4228.511 kb on + strand, within yjbCat 4228.547 kb on + strand, within yjbCat 4228.555 kb on - strand, within yjbCat 4228.555 kb on - strand, within yjbCat 4228.555 kb on - strand, within yjbCat 4228.605 kb on - strand, within yjbCat 4228.610 kb on + strand, within yjbCat 4228.688 kb on - strand, within yjbCat 4228.695 kb on + strand, within yjbCat 4228.703 kb on - strand, within yjbCat 4228.737 kb on + strand, within yjbCat 4228.737 kb on + strand, within yjbCat 4228.786 kb on + strand, within yjbCat 4228.798 kb on - strand, within yjbCat 4228.835 kb on + strand, within yjbCat 4228.837 kb on + strand, within yjbCat 4228.837 kb on + strand, within yjbCat 4228.845 kb on - strand, within yjbCat 4228.861 kb on - strand, within yjbCat 4228.892 kb on + strand, within yjbCat 4228.892 kb on + strand, within yjbCat 4228.909 kb on + strand, within yjbCat 4228.915 kb on + strand, within yjbCat 4228.920 kb on - strand, within yjbCat 4228.923 kb on - strand, within yjbCat 4228.923 kb on - strand, within yjbCat 4228.929 kb on - strand, within yjbCat 4229.003 kb on - strand, within yjbCat 4229.003 kb on - strand, within yjbCat 4229.010 kb on + strand, within yjbCat 4229.010 kb on + strand, within yjbCat 4229.020 kb on + strand, within yjbCat 4229.020 kb on + strand, within yjbCat 4229.071 kb on + strand, within yjbCat 4229.072 kb on + strand, within yjbCat 4229.080 kb on - strand, within yjbCat 4229.080 kb on - strand, within yjbCat 4229.080 kb on - strand, within yjbCat 4229.154 kb on + strand, within yjbCat 4229.162 kb on - strandat 4229.255 kb on + strandat 4229.263 kb on - strandat 4229.263 kb on - strandat 4229.298 kb on - strandat 4229.298 kb on - strandat 4229.336 kb on + strandat 4229.421 kb on - strand, within yjbDat 4229.533 kb on + strand, within yjbDat 4229.539 kb on + strand, within yjbDat 4229.551 kb on + strand, within yjbDat 4229.555 kb on + strand, within yjbDat 4229.555 kb on + strand, within yjbDat 4229.563 kb on - strand, within yjbDat 4229.563 kb on - strand, within yjbDat 4229.563 kb on - strand, within yjbDat 4229.572 kb on + strand, within yjbDat 4229.583 kb on + strand, within yjbDat 4229.583 kb on + strand, within yjbDat 4229.591 kb on + strand, within yjbDat 4229.599 kb on + strand, within yjbDat 4229.603 kb on - strand, within yjbDat 4229.603 kb on - strand, within yjbDat 4229.609 kb on + strand, within yjbDat 4229.620 kb on + strand, within yjbDat 4229.638 kb on + strandat 4229.638 kb on + strandat 4229.638 kb on + strandat 4229.646 kb on - strandat 4229.648 kb on - strandat 4229.648 kb on - strandat 4229.650 kb on + strandat 4229.650 kb on + strandat 4229.650 kb on + strandat 4229.650 kb on + strandat 4229.651 kb on + strandat 4229.651 kb on + strandat 4229.659 kb on - strandat 4229.661 kb on + strandat 4229.663 kb on + strandat 4229.666 kb on + strandat 4229.668 kb on - strandat 4229.678 kb on + strandat 4229.693 kb on - strandat 4229.695 kb on - strandat 4229.734 kb on + strandat 4229.796 kb on + strandat 4229.818 kb on + strandat 4229.821 kb on - strandat 4229.826 kb on + strandat 4229.826 kb on - strandat 4229.831 kb on - strandat 4229.831 kb on - strandat 4229.831 kb on - strandat 4229.835 kb on - strandat 4229.836 kb on - strandat 4229.836 kb on - strandat 4229.836 kb on - strandat 4229.836 kb on - strandat 4229.845 kb on + strandat 4229.898 kb on + strandat 4229.898 kb on + strandat 4229.903 kb on + strandat 4229.903 kb on + strandat 4229.903 kb on + strandat 4229.910 kb on - strandat 4229.912 kb on - strandat 4229.951 kb on - strandat 4229.951 kb on - strandat 4229.953 kb on - strandat 4229.953 kb on - strandat 4229.964 kb on - strandat 4229.973 kb on + strandat 4229.973 kb on + strandat 4229.974 kb on - strandat 4229.978 kb on + strandat 4229.986 kb on - strandat 4229.986 kb on - strandat 4229.986 kb on - strandat 4229.997 kb on - strandat 4230.031 kb on - strandat 4230.031 kb on - strandat 4230.061 kb on + strand, within lysCat 4230.084 kb on - strand, within lysCat 4230.126 kb on - strand, within lysCat 4230.182 kb on - strand, within lysCat 4230.182 kb on - strand, within lysCat 4230.187 kb on - strand, within lysCat 4230.226 kb on - strand, within lysCat 4230.226 kb on - strand, within lysCat 4230.226 kb on - strand, within lysCat 4230.226 kb on - strand, within lysCat 4230.229 kb on + strand, within lysCat 4230.237 kb on - strand, within lysC

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml
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4,227,407 + +0.8
4,227,427 + +1.1
4,227,462 - -1.3
4,227,509 + +1.1
4,227,509 + -0.7
4,227,527 - +1.7
4,227,527 - -0.3
4,227,534 - +1.2
4,227,551 - pepE b4021 0.11 -0.3
4,227,556 - pepE b4021 0.12 -0.2
4,227,556 - pepE b4021 0.12 -0.2
4,227,621 - pepE b4021 0.21 -0.8
4,227,626 + pepE b4021 0.22 +0.1
4,227,626 + pepE b4021 0.22 +0.1
4,227,634 + pepE b4021 0.23 +0.7
4,227,634 + pepE b4021 0.23 -1.0
4,227,657 - pepE b4021 0.26 +0.4
4,227,739 + pepE b4021 0.38 -0.3
4,227,739 + pepE b4021 0.38 +4.0
4,227,772 + pepE b4021 0.43 +1.0
4,227,796 - pepE b4021 0.46 +1.4
4,227,796 - pepE b4021 0.46 +2.0
4,227,813 + pepE b4021 0.49 -0.4
4,227,821 - pepE b4021 0.50 +1.1
4,227,887 - pepE b4021 0.60 -0.6
4,227,887 - pepE b4021 0.60 -1.3
4,227,888 - pepE b4021 0.60 -1.9
4,227,888 - pepE b4021 0.60 -1.2
4,227,993 + pepE b4021 0.75 -0.2
4,228,039 - pepE b4021 0.82 -0.9
4,228,077 + pepE b4021 0.87 +0.7
4,228,087 - pepE b4021 0.89 -1.1
4,228,087 - pepE b4021 0.89 +0.4
4,228,118 - -1.6
4,228,118 - +1.4
4,228,119 - -0.8
4,228,125 - -0.0
4,228,125 - +0.3
4,228,159 + +0.7
4,228,162 + +0.9
4,228,162 + -0.4
4,228,200 + +0.2
4,228,200 + -2.0
4,228,223 + +3.4
4,228,223 + +1.0
4,228,225 + -2.0
4,228,225 + -1.6
4,228,225 + -0.9
4,228,231 - -0.1
4,228,231 - +1.4
4,228,231 - +1.1
4,228,235 + +2.3
4,228,235 + +1.9
4,228,235 + +1.4
4,228,252 - -1.1
4,228,267 + +2.3
4,228,303 + +1.2
4,228,344 - +2.7
4,228,344 - +2.1
4,228,369 - +1.7
4,228,369 - +3.0
4,228,387 + +2.3
4,228,451 + -0.1
4,228,451 + -0.1
4,228,451 + -2.9
4,228,451 + +0.1
4,228,459 - -0.6
4,228,459 - -1.3
4,228,459 - -2.2
4,228,461 + +1.4
4,228,478 + yjbC b4022 0.12 +1.5
4,228,478 - yjbC b4022 0.12 +2.2
4,228,488 - yjbC b4022 0.13 +1.1
4,228,498 + yjbC b4022 0.14 +1.6
4,228,511 + yjbC b4022 0.15 +1.4
4,228,547 + yjbC b4022 0.19 -1.6
4,228,555 - yjbC b4022 0.20 -1.3
4,228,555 - yjbC b4022 0.20 -0.3
4,228,555 - yjbC b4022 0.20 +1.0
4,228,605 - yjbC b4022 0.26 +0.2
4,228,610 + yjbC b4022 0.27 -0.3
4,228,688 - yjbC b4022 0.36 -0.5
4,228,695 + yjbC b4022 0.36 -0.6
4,228,703 - yjbC b4022 0.37 -1.3
4,228,737 + yjbC b4022 0.41 +1.7
4,228,737 + yjbC b4022 0.41 +2.7
4,228,786 + yjbC b4022 0.47 -0.4
4,228,798 - yjbC b4022 0.48 +0.4
4,228,835 + yjbC b4022 0.52 -2.0
4,228,837 + yjbC b4022 0.53 +1.9
4,228,837 + yjbC b4022 0.53 -1.5
4,228,845 - yjbC b4022 0.54 -0.5
4,228,861 - yjbC b4022 0.55 -2.0
4,228,892 + yjbC b4022 0.59 -1.6
4,228,892 + yjbC b4022 0.59 +2.6
4,228,909 + yjbC b4022 0.61 +1.1
4,228,915 + yjbC b4022 0.62 +0.1
4,228,920 - yjbC b4022 0.62 -1.5
4,228,923 - yjbC b4022 0.63 +0.1
4,228,923 - yjbC b4022 0.63 +1.3
4,228,929 - yjbC b4022 0.63 -0.3
4,229,003 - yjbC b4022 0.72 -0.4
4,229,003 - yjbC b4022 0.72 -2.0
4,229,010 + yjbC b4022 0.73 +0.2
4,229,010 + yjbC b4022 0.73 -0.8
4,229,020 + yjbC b4022 0.74 -1.3
4,229,020 + yjbC b4022 0.74 +2.3
4,229,071 + yjbC b4022 0.79 +0.5
4,229,072 + yjbC b4022 0.80 +1.2
4,229,080 - yjbC b4022 0.81 +1.9
4,229,080 - yjbC b4022 0.81 -1.1
4,229,080 - yjbC b4022 0.81 -1.2
4,229,154 + yjbC b4022 0.89 -0.7
4,229,162 - -0.9
4,229,255 + -2.0
4,229,263 - +1.5
4,229,263 - +0.7
4,229,298 - -1.1
4,229,298 - +1.0
4,229,336 + +1.2
4,229,421 - yjbD b4023 0.14 -1.9
4,229,533 + yjbD b4023 0.55 -1.1
4,229,539 + yjbD b4023 0.58 -0.4
4,229,551 + yjbD b4023 0.62 +0.4
4,229,555 + yjbD b4023 0.63 -1.4
4,229,555 + yjbD b4023 0.63 +1.6
4,229,563 - yjbD b4023 0.66 +1.7
4,229,563 - yjbD b4023 0.66 +0.1
4,229,563 - yjbD b4023 0.66 +0.1
4,229,572 + yjbD b4023 0.70 +1.2
4,229,583 + yjbD b4023 0.74 +0.7
4,229,583 + yjbD b4023 0.74 +0.7
4,229,591 + yjbD b4023 0.77 -2.2
4,229,599 + yjbD b4023 0.79 -0.3
4,229,603 - yjbD b4023 0.81 -0.0
4,229,603 - yjbD b4023 0.81 -2.5
4,229,609 + yjbD b4023 0.83 +0.8
4,229,620 + yjbD b4023 0.87 +1.3
4,229,638 + -1.5
4,229,638 + -0.8
4,229,638 + -1.3
4,229,646 - -0.5
4,229,648 - +0.9
4,229,648 - +0.6
4,229,650 + +1.3
4,229,650 + -0.9
4,229,650 + -1.0
4,229,650 + +1.7
4,229,651 + -1.0
4,229,651 + +1.7
4,229,659 - -0.3
4,229,661 + +0.7
4,229,663 + -0.7
4,229,666 + -0.9
4,229,668 - +1.0
4,229,678 + -0.8
4,229,693 - -2.5
4,229,695 - -1.5
4,229,734 + -1.6
4,229,796 + +0.3
4,229,818 + -0.9
4,229,821 - -1.1
4,229,826 + -1.6
4,229,826 - -0.3
4,229,831 - +0.5
4,229,831 - +1.7
4,229,831 - +1.5
4,229,835 - +0.2
4,229,836 - -0.3
4,229,836 - -1.3
4,229,836 - +0.2
4,229,836 - -0.6
4,229,845 + -0.6
4,229,898 + -2.2
4,229,898 + -1.8
4,229,903 + -0.3
4,229,903 + -1.3
4,229,903 + -0.1
4,229,910 - +1.2
4,229,912 - -1.0
4,229,951 - +0.4
4,229,951 - +0.7
4,229,953 - +0.7
4,229,953 - +1.7
4,229,964 - -0.4
4,229,973 + +2.1
4,229,973 + -0.6
4,229,974 - -0.3
4,229,978 + +0.8
4,229,986 - -1.1
4,229,986 - -2.3
4,229,986 - +4.3
4,229,997 - +0.1
4,230,031 - +0.4
4,230,031 - -1.8
4,230,061 + lysC b4024 0.11 +2.1
4,230,084 - lysC b4024 0.13 -0.3
4,230,126 - lysC b4024 0.16 -0.5
4,230,182 - lysC b4024 0.20 -2.2
4,230,182 - lysC b4024 0.20 +1.9
4,230,187 - lysC b4024 0.21 -1.6
4,230,226 - lysC b4024 0.24 -0.9
4,230,226 - lysC b4024 0.24 -1.5
4,230,226 - lysC b4024 0.24 -1.5
4,230,226 - lysC b4024 0.24 -1.8
4,230,229 + lysC b4024 0.24 -0.8
4,230,237 - lysC b4024 0.24 +1.3

Or see this region's nucleotide sequence