Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaQ and kdtA are separated by 441 nucleotides kdtA and coaD are separated by 7 nucleotides coaD and mutM are separated by 38 nucleotides
b3632: rfaQ - lipopolysaccharide core biosynthesis protein (NCBI), at 3,805,087 to 3,806,121
rfaQ
b3633: kdtA - 3-deoxy-D-manno-octulosonic-acid transferase (NCBI), at 3,806,563 to 3,807,840
kdtA
b3634: coaD - phosphopantetheine adenylyltransferase (NCBI), at 3,807,848 to 3,808,327
coaD
b3635: mutM - formamidopyrimidine-DNA glycosylase (NCBI), at 3,808,366 to 3,809,175
mutM
Position (kb)
3806
3807
3808 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 3805.582 kb on - strand, within rfaQ at 3805.584 kb on + strand, within rfaQ at 3805.591 kb on - strand, within rfaQ at 3805.594 kb on - strand, within rfaQ at 3805.594 kb on - strand, within rfaQ at 3805.602 kb on - strand, within rfaQ at 3805.625 kb on - strand, within rfaQ at 3805.625 kb on - strand, within rfaQ at 3805.650 kb on - strand, within rfaQ at 3805.655 kb on - strand, within rfaQ at 3805.655 kb on - strand, within rfaQ at 3805.673 kb on + strand, within rfaQ at 3805.676 kb on + strand, within rfaQ at 3805.676 kb on + strand, within rfaQ at 3805.682 kb on - strand, within rfaQ at 3805.693 kb on - strand, within rfaQ at 3805.693 kb on - strand, within rfaQ at 3805.693 kb on - strand, within rfaQ at 3805.693 kb on - strand, within rfaQ at 3805.695 kb on + strand, within rfaQ at 3805.700 kb on - strand, within rfaQ at 3805.700 kb on - strand, within rfaQ at 3805.703 kb on - strand, within rfaQ at 3805.705 kb on - strand, within rfaQ at 3805.705 kb on - strand, within rfaQ at 3805.719 kb on - strand, within rfaQ at 3805.765 kb on + strand, within rfaQ at 3805.765 kb on + strand, within rfaQ at 3805.765 kb on - strand, within rfaQ at 3805.769 kb on - strand, within rfaQ at 3805.769 kb on - strand, within rfaQ at 3805.773 kb on - strand, within rfaQ at 3805.795 kb on - strand, within rfaQ at 3805.795 kb on - strand, within rfaQ at 3805.805 kb on - strand, within rfaQ at 3805.805 kb on - strand, within rfaQ at 3805.805 kb on - strand, within rfaQ at 3805.808 kb on - strand, within rfaQ at 3805.835 kb on + strand, within rfaQ at 3805.841 kb on - strand, within rfaQ at 3805.841 kb on - strand, within rfaQ at 3805.843 kb on + strand, within rfaQ at 3805.843 kb on + strand, within rfaQ at 3805.843 kb on + strand, within rfaQ at 3805.843 kb on - strand, within rfaQ at 3805.851 kb on - strand, within rfaQ at 3805.852 kb on - strand, within rfaQ at 3805.852 kb on - strand, within rfaQ at 3805.852 kb on - strand, within rfaQ at 3805.854 kb on - strand, within rfaQ at 3805.854 kb on - strand, within rfaQ at 3805.854 kb on - strand, within rfaQ at 3805.854 kb on - strand, within rfaQ at 3805.867 kb on - strand, within rfaQ at 3805.867 kb on - strand, within rfaQ at 3805.867 kb on - strand, within rfaQ at 3805.872 kb on - strand, within rfaQ at 3805.872 kb on - strand, within rfaQ at 3805.872 kb on - strand, within rfaQ at 3805.879 kb on + strand, within rfaQ at 3805.887 kb on - strand, within rfaQ at 3805.887 kb on - strand, within rfaQ at 3805.890 kb on + strand, within rfaQ at 3805.890 kb on + strand, within rfaQ at 3805.895 kb on + strand, within rfaQ at 3805.898 kb on - strand, within rfaQ at 3805.898 kb on - strand, within rfaQ at 3805.900 kb on - strand, within rfaQ at 3805.973 kb on + strand, within rfaQ at 3805.981 kb on - strand, within rfaQ at 3805.981 kb on - strand, within rfaQ at 3805.984 kb on - strand, within rfaQ at 3805.984 kb on - strand, within rfaQ at 3805.984 kb on - strand, within rfaQ at 3806.026 kb on - strand at 3806.026 kb on - strand at 3806.026 kb on - strand at 3806.026 kb on - strand at 3806.033 kb on - strand at 3806.035 kb on - strand at 3806.038 kb on - strand at 3806.070 kb on - strand at 3806.070 kb on - strand at 3806.078 kb on - strand at 3806.083 kb on - strand at 3806.083 kb on - strand at 3806.088 kb on - strand at 3806.088 kb on - strand at 3806.096 kb on - strand at 3806.096 kb on - strand at 3806.096 kb on - strand at 3806.101 kb on - strand at 3806.101 kb on - strand at 3806.101 kb on - strand at 3806.101 kb on - strand at 3806.108 kb on - strand at 3806.162 kb on - strand at 3806.167 kb on - strand at 3806.177 kb on - strand at 3806.177 kb on - strand at 3806.222 kb on + strand at 3806.222 kb on + strand at 3806.241 kb on - strand at 3806.241 kb on - strand at 3806.263 kb on - strand at 3806.274 kb on - strand at 3806.274 kb on - strand at 3806.277 kb on - strand at 3806.381 kb on - strand at 3806.387 kb on - strand at 3806.560 kb on + strand at 3806.568 kb on - strand at 3806.568 kb on - strand at 3806.568 kb on - strand at 3806.574 kb on + strand at 3808.353 kb on - strand at 3808.433 kb on + strand at 3808.433 kb on + strand at 3808.443 kb on - strand at 3808.450 kb on - strand, within mutM at 3808.497 kb on + strand, within mutM at 3808.532 kb on + strand, within mutM at 3808.535 kb on - strand, within mutM at 3808.540 kb on - strand, within mutM at 3808.558 kb on - strand, within mutM at 3808.563 kb on - strand, within mutM at 3808.565 kb on + strand, within mutM at 3808.614 kb on - strand, within mutM at 3808.614 kb on - strand, within mutM at 3808.699 kb on - strand, within mutM at 3808.738 kb on + strand, within mutM at 3808.753 kb on - strand, within mutM at 3808.757 kb on + strand, within mutM at 3808.758 kb on - strand, within mutM at 3808.766 kb on - strand, within mutM at 3808.784 kb on + strand, within mutM at 3808.793 kb on - strand, within mutM at 3808.796 kb on - strand, within mutM at 3808.810 kb on - strand, within mutM at 3808.824 kb on - strand, within mutM at 3808.826 kb on + strand, within mutM at 3808.833 kb on + strand, within mutM at 3808.833 kb on + strand, within mutM at 3808.834 kb on - strand, within mutM at 3808.834 kb on - strand, within mutM
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 3,805,582 - rfaQ b3632 0.48 -0.6 3,805,584 + rfaQ b3632 0.48 -1.5 3,805,591 - rfaQ b3632 0.49 +0.3 3,805,594 - rfaQ b3632 0.49 -1.7 3,805,594 - rfaQ b3632 0.49 -1.7 3,805,602 - rfaQ b3632 0.50 -1.5 3,805,625 - rfaQ b3632 0.52 -1.6 3,805,625 - rfaQ b3632 0.52 -1.0 3,805,650 - rfaQ b3632 0.54 -0.9 3,805,655 - rfaQ b3632 0.55 -0.5 3,805,655 - rfaQ b3632 0.55 -2.6 3,805,673 + rfaQ b3632 0.57 -1.7 3,805,676 + rfaQ b3632 0.57 -0.2 3,805,676 + rfaQ b3632 0.57 -0.5 3,805,682 - rfaQ b3632 0.57 +0.3 3,805,693 - rfaQ b3632 0.59 -1.9 3,805,693 - rfaQ b3632 0.59 -0.5 3,805,693 - rfaQ b3632 0.59 -2.0 3,805,693 - rfaQ b3632 0.59 -0.7 3,805,695 + rfaQ b3632 0.59 -1.4 3,805,700 - rfaQ b3632 0.59 -1.6 3,805,700 - rfaQ b3632 0.59 -2.4 3,805,703 - rfaQ b3632 0.60 -2.9 3,805,705 - rfaQ b3632 0.60 +0.3 3,805,705 - rfaQ b3632 0.60 -1.2 3,805,719 - rfaQ b3632 0.61 -2.6 3,805,765 + rfaQ b3632 0.66 -0.7 3,805,765 + rfaQ b3632 0.66 -1.3 3,805,765 - rfaQ b3632 0.66 -2.8 3,805,769 - rfaQ b3632 0.66 -0.2 3,805,769 - rfaQ b3632 0.66 -1.0 3,805,773 - rfaQ b3632 0.66 -2.7 3,805,795 - rfaQ b3632 0.68 -1.0 3,805,795 - rfaQ b3632 0.68 -1.9 3,805,805 - rfaQ b3632 0.69 -2.6 3,805,805 - rfaQ b3632 0.69 -1.4 3,805,805 - rfaQ b3632 0.69 -2.7 3,805,808 - rfaQ b3632 0.70 -2.4 3,805,835 + rfaQ b3632 0.72 -1.3 3,805,841 - rfaQ b3632 0.73 -2.5 3,805,841 - rfaQ b3632 0.73 -1.2 3,805,843 + rfaQ b3632 0.73 -2.6 3,805,843 + rfaQ b3632 0.73 -2.6 3,805,843 + rfaQ b3632 0.73 -2.0 3,805,843 - rfaQ b3632 0.73 -3.7 3,805,851 - rfaQ b3632 0.74 -1.9 3,805,852 - rfaQ b3632 0.74 -1.5 3,805,852 - rfaQ b3632 0.74 -0.5 3,805,852 - rfaQ b3632 0.74 +1.8 3,805,854 - rfaQ b3632 0.74 -0.5 3,805,854 - rfaQ b3632 0.74 -0.2 3,805,854 - rfaQ b3632 0.74 -1.6 3,805,854 - rfaQ b3632 0.74 -1.2 3,805,867 - rfaQ b3632 0.75 -1.6 3,805,867 - rfaQ b3632 0.75 -1.3 3,805,867 - rfaQ b3632 0.75 -2.4 3,805,872 - rfaQ b3632 0.76 -1.6 3,805,872 - rfaQ b3632 0.76 -2.1 3,805,872 - rfaQ b3632 0.76 -1.1 3,805,879 + rfaQ b3632 0.77 -1.0 3,805,887 - rfaQ b3632 0.77 -1.3 3,805,887 - rfaQ b3632 0.77 -1.4 3,805,890 + rfaQ b3632 0.78 -1.3 3,805,890 + rfaQ b3632 0.78 -0.7 3,805,895 + rfaQ b3632 0.78 -0.7 3,805,898 - rfaQ b3632 0.78 -1.5 3,805,898 - rfaQ b3632 0.78 -2.1 3,805,900 - rfaQ b3632 0.79 -1.0 3,805,973 + rfaQ b3632 0.86 +0.3 3,805,981 - rfaQ b3632 0.86 -2.1 3,805,981 - rfaQ b3632 0.86 -1.5 3,805,984 - rfaQ b3632 0.87 +0.8 3,805,984 - rfaQ b3632 0.87 +0.3 3,805,984 - rfaQ b3632 0.87 -0.2 3,806,026 - -2.7 3,806,026 - -2.2 3,806,026 - -2.7 3,806,026 - -0.7 3,806,033 - -2.2 3,806,035 - -2.2 3,806,038 - -1.5 3,806,070 - +0.8 3,806,070 - +0.3 3,806,078 - -1.5 3,806,083 - -1.0 3,806,083 - +1.8 3,806,088 - -1.3 3,806,088 - -1.9 3,806,096 - -1.7 3,806,096 - -2.5 3,806,096 - -2.4 3,806,101 - -2.8 3,806,101 - -0.7 3,806,101 - +0.3 3,806,101 - +0.8 3,806,108 - -1.9 3,806,162 - -0.5 3,806,167 - +4.9 3,806,177 - +2.6 3,806,177 - +0.6 3,806,222 + -1.7 3,806,222 + -2.3 3,806,241 - -0.7 3,806,241 - +0.4 3,806,263 - +5.9 3,806,274 - +4.0 3,806,274 - +4.0 3,806,277 - +4.7 3,806,381 - +4.6 3,806,387 - +4.2 3,806,560 + -1.5 3,806,568 - +3.3 3,806,568 - +4.6 3,806,568 - +2.6 3,806,574 + -1.5 3,808,353 - -2.2 3,808,433 + -0.7 3,808,433 + +1.2 3,808,443 - +1.5 3,808,450 - mutM b3635 0.10 +1.0 3,808,497 + mutM b3635 0.16 -2.5 3,808,532 + mutM b3635 0.20 +1.8 3,808,535 - mutM b3635 0.21 +2.8 3,808,540 - mutM b3635 0.21 -0.0 3,808,558 - mutM b3635 0.24 -1.3 3,808,563 - mutM b3635 0.24 +1.8 3,808,565 + mutM b3635 0.25 -2.4 3,808,614 - mutM b3635 0.31 +0.1 3,808,614 - mutM b3635 0.31 -0.6 3,808,699 - mutM b3635 0.41 -1.2 3,808,738 + mutM b3635 0.46 -0.5 3,808,753 - mutM b3635 0.48 +3.1 3,808,757 + mutM b3635 0.48 -0.6 3,808,758 - mutM b3635 0.48 +1.6 3,808,766 - mutM b3635 0.49 -0.2 3,808,784 + mutM b3635 0.52 +1.4 3,808,793 - mutM b3635 0.53 -1.1 3,808,796 - mutM b3635 0.53 +1.0 3,808,810 - mutM b3635 0.55 +0.1 3,808,824 - mutM b3635 0.57 -1.2 3,808,826 + mutM b3635 0.57 -2.6 3,808,833 + mutM b3635 0.58 -0.8 3,808,833 + mutM b3635 0.58 -1.5 3,808,834 - mutM b3635 0.58 +0.3 3,808,834 - mutM b3635 0.58 -0.2
Or see this region's nucleotide sequence