Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bcsE and yhjT overlap by 4 nucleotides yhjT and bcsG overlap by 4 nucleotides bcsG and ldrD are separated by 86 nucleotides ldrD and rdlD are separated by 48 nucleotides rdlD and yhjV are separated by 361 nucleotides
b3536: bcsE - hypothetical protein (NCBI), at 3,694,481 to 3,696,052
bcsE
b3537: yhjT - orf, hypothetical protein (VIMSS), at 3,696,049 to 3,696,240
yhjT
b3538: bcsG - predicted inner membrane protein (NCBI), at 3,696,237 to 3,697,916
bcsG
b4453: ldrD - toxic polypeptide, small (NCBI), at 3,698,003 to 3,698,110
ldrD
b4454: rdlD - sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR-D repeat, with toxic peptide LdrD (RefSeq), at 3,698,159 to 3,698,224
rdlD
b3539: yhjV - predicted transporter (NCBI), at 3,698,586 to 3,699,857
yhjV
Position (kb)
3696
3697
3698 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3695.251 kb on + strand, within bcsE at 3695.251 kb on + strand, within bcsE at 3695.251 kb on + strand, within bcsE at 3695.305 kb on + strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.336 kb on - strand, within bcsE at 3695.352 kb on + strand, within bcsE at 3695.385 kb on - strand, within bcsE at 3695.460 kb on - strand, within bcsE at 3695.462 kb on - strand, within bcsE at 3695.462 kb on - strand, within bcsE at 3695.472 kb on + strand, within bcsE at 3695.480 kb on - strand, within bcsE at 3695.480 kb on - strand, within bcsE at 3695.520 kb on + strand, within bcsE at 3695.567 kb on - strand, within bcsE at 3695.567 kb on - strand, within bcsE at 3695.581 kb on + strand, within bcsE at 3695.582 kb on - strand, within bcsE at 3695.583 kb on + strand, within bcsE at 3695.591 kb on + strand, within bcsE at 3695.596 kb on + strand, within bcsE at 3695.632 kb on + strand, within bcsE at 3695.637 kb on - strand, within bcsE at 3695.637 kb on - strand, within bcsE at 3695.638 kb on - strand, within bcsE at 3695.644 kb on + strand, within bcsE at 3695.644 kb on + strand, within bcsE at 3695.700 kb on + strand, within bcsE at 3695.757 kb on + strand, within bcsE at 3695.814 kb on + strand, within bcsE at 3695.871 kb on - strand, within bcsE at 3695.894 kb on + strand, within bcsE at 3695.902 kb on - strand at 3695.902 kb on - strand at 3695.902 kb on - strand at 3695.926 kb on + strand at 3695.928 kb on - strand at 3695.938 kb on + strand at 3695.952 kb on + strand at 3695.952 kb on + strand at 3695.965 kb on + strand at 3695.985 kb on + strand at 3695.994 kb on + strand at 3696.022 kb on + strand at 3696.022 kb on + strand at 3696.032 kb on + strand at 3696.124 kb on - strand, within yhjT at 3696.124 kb on - strand, within yhjT at 3696.131 kb on + strand, within yhjT at 3696.131 kb on + strand, within yhjT at 3696.199 kb on + strand, within yhjT at 3696.205 kb on - strand, within yhjT at 3696.268 kb on + strand at 3696.292 kb on - strand at 3696.338 kb on + strand at 3696.338 kb on + strand at 3696.346 kb on - strand at 3696.346 kb on - strand at 3696.352 kb on + strand at 3696.416 kb on + strand, within bcsG at 3696.416 kb on + strand, within bcsG at 3696.416 kb on + strand, within bcsG at 3696.431 kb on - strand, within bcsG at 3696.445 kb on - strand, within bcsG at 3696.450 kb on - strand, within bcsG at 3696.473 kb on - strand, within bcsG at 3696.473 kb on - strand, within bcsG at 3696.473 kb on - strand, within bcsG at 3696.504 kb on - strand, within bcsG at 3696.519 kb on + strand, within bcsG at 3696.544 kb on - strand, within bcsG at 3696.581 kb on - strand, within bcsG at 3696.630 kb on - strand, within bcsG at 3696.677 kb on - strand, within bcsG at 3696.677 kb on - strand, within bcsG at 3696.681 kb on - strand, within bcsG at 3696.714 kb on + strand, within bcsG at 3696.753 kb on - strand, within bcsG at 3696.753 kb on - strand, within bcsG at 3696.771 kb on - strand, within bcsG at 3696.771 kb on - strand, within bcsG at 3696.804 kb on + strand, within bcsG at 3696.804 kb on + strand, within bcsG at 3696.829 kb on - strand, within bcsG at 3696.833 kb on + strand, within bcsG at 3696.833 kb on + strand, within bcsG at 3696.851 kb on + strand, within bcsG at 3696.925 kb on + strand, within bcsG at 3696.950 kb on + strand, within bcsG at 3696.980 kb on + strand at 3696.989 kb on - strand, within bcsG at 3697.005 kb on - strand, within bcsG at 3697.005 kb on - strand, within bcsG at 3697.052 kb on + strand, within bcsG at 3697.061 kb on + strand, within bcsG at 3697.062 kb on + strand, within bcsG at 3697.067 kb on - strand, within bcsG at 3697.069 kb on - strand, within bcsG at 3697.072 kb on + strand, within bcsG at 3697.084 kb on + strand, within bcsG at 3697.116 kb on + strand, within bcsG at 3697.120 kb on + strand, within bcsG at 3697.128 kb on - strand, within bcsG at 3697.138 kb on - strand, within bcsG at 3697.326 kb on + strand, within bcsG at 3697.336 kb on + strand, within bcsG at 3697.339 kb on + strand, within bcsG at 3697.339 kb on + strand, within bcsG at 3697.344 kb on + strand, within bcsG at 3697.352 kb on - strand, within bcsG at 3697.395 kb on - strand, within bcsG at 3697.395 kb on - strand, within bcsG at 3697.401 kb on - strand, within bcsG at 3697.401 kb on - strand, within bcsG at 3697.408 kb on + strand, within bcsG at 3697.419 kb on - strand, within bcsG at 3697.433 kb on - strand, within bcsG at 3697.441 kb on - strand, within bcsG at 3697.441 kb on - strand, within bcsG at 3697.547 kb on - strand, within bcsG at 3697.560 kb on + strand, within bcsG at 3697.568 kb on - strand, within bcsG at 3697.570 kb on - strand, within bcsG at 3697.607 kb on + strand, within bcsG at 3697.607 kb on + strand, within bcsG at 3697.615 kb on + strand, within bcsG at 3697.615 kb on + strand, within bcsG at 3697.677 kb on + strand, within bcsG at 3697.736 kb on + strand, within bcsG at 3697.861 kb on + strand at 3697.886 kb on - strand at 3697.886 kb on - strand at 3697.945 kb on + strand at 3697.945 kb on + strand at 3698.018 kb on + strand, within ldrD at 3698.052 kb on + strand, within ldrD at 3698.055 kb on - strand, within ldrD at 3698.103 kb on - strand at 3698.103 kb on - strand at 3698.107 kb on + strand at 3698.158 kb on + strand at 3698.158 kb on + strand at 3698.158 kb on + strand at 3698.158 kb on + strand at 3698.165 kb on + strand at 3698.165 kb on + strand at 3698.166 kb on - strand, within rdlD at 3698.178 kb on - strand, within rdlD at 3698.185 kb on + strand, within rdlD at 3698.190 kb on + strand, within rdlD at 3698.195 kb on + strand, within rdlD at 3698.195 kb on + strand, within rdlD at 3698.195 kb on + strand, within rdlD at 3698.195 kb on + strand, within rdlD at 3698.257 kb on - strand at 3698.260 kb on + strand at 3698.277 kb on + strand at 3698.318 kb on + strand at 3698.333 kb on + strand at 3698.333 kb on + strand at 3698.340 kb on + strand at 3698.407 kb on - strand at 3698.410 kb on + strand at 3698.410 kb on + strand at 3698.410 kb on + strand at 3698.410 kb on + strand at 3698.411 kb on + strand at 3698.418 kb on - strand at 3698.418 kb on - strand at 3698.418 kb on - strand at 3698.429 kb on - strand at 3698.429 kb on - strand at 3698.429 kb on - strand at 3698.452 kb on + strand at 3698.452 kb on - strand at 3698.473 kb on + strand at 3698.474 kb on - strand at 3698.474 kb on - strand at 3698.487 kb on + strand at 3698.487 kb on + strand at 3698.501 kb on + strand at 3698.501 kb on + strand at 3698.503 kb on + strand at 3698.503 kb on + strand at 3698.507 kb on - strand at 3698.519 kb on - strand at 3698.533 kb on - strand at 3698.533 kb on - strand at 3698.533 kb on - strand at 3698.589 kb on - strand at 3698.591 kb on + strand at 3698.591 kb on + strand at 3698.593 kb on + strand at 3698.607 kb on - strand at 3698.612 kb on - strand at 3698.632 kb on + strand at 3698.632 kb on + strand at 3698.634 kb on + strand at 3698.640 kb on + strand at 3698.642 kb on - strand at 3698.660 kb on + strand at 3698.660 kb on + strand at 3698.667 kb on + strand at 3698.681 kb on + strand at 3698.681 kb on + strand at 3698.683 kb on + strand at 3698.683 kb on + strand at 3698.689 kb on + strand at 3698.690 kb on - strand at 3698.691 kb on + strand at 3698.691 kb on + strand at 3698.693 kb on + strand at 3698.694 kb on - strand at 3698.696 kb on - strand at 3698.696 kb on - strand at 3698.699 kb on - strand at 3698.699 kb on - strand at 3698.699 kb on - strand at 3698.701 kb on - strand at 3698.715 kb on - strand, within yhjV at 3698.755 kb on - strand, within yhjV at 3698.760 kb on + strand, within yhjV at 3698.760 kb on - strand, within yhjV at 3698.760 kb on - strand, within yhjV at 3698.761 kb on - strand, within yhjV at 3698.761 kb on - strand, within yhjV at 3698.765 kb on + strand, within yhjV at 3698.765 kb on + strand, within yhjV at 3698.765 kb on + strand, within yhjV at 3698.765 kb on + strand, within yhjV at 3698.765 kb on + strand, within yhjV at 3698.773 kb on - strand, within yhjV at 3698.773 kb on - strand, within yhjV at 3698.773 kb on - strand, within yhjV at 3698.773 kb on - strand, within yhjV at 3698.775 kb on + strand, within yhjV at 3698.783 kb on + strand, within yhjV at 3698.783 kb on - strand, within yhjV at 3698.783 kb on - strand, within yhjV at 3698.783 kb on - strand, within yhjV at 3698.822 kb on + strand, within yhjV at 3698.847 kb on + strand, within yhjV at 3698.850 kb on - strand, within yhjV at 3698.850 kb on - strand, within yhjV at 3698.856 kb on - strand, within yhjV at 3698.909 kb on - strand, within yhjV at 3698.909 kb on - strand, within yhjV
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 3,695,251 + bcsE b3536 0.49 -1.6 3,695,251 + bcsE b3536 0.49 +0.6 3,695,251 + bcsE b3536 0.49 +0.8 3,695,305 + bcsE b3536 0.52 +0.9 3,695,336 - bcsE b3536 0.54 +2.3 3,695,336 - bcsE b3536 0.54 +0.3 3,695,336 - bcsE b3536 0.54 +0.8 3,695,336 - bcsE b3536 0.54 -0.3 3,695,336 - bcsE b3536 0.54 -1.8 3,695,336 - bcsE b3536 0.54 -0.8 3,695,352 + bcsE b3536 0.55 +0.6 3,695,385 - bcsE b3536 0.58 +2.8 3,695,460 - bcsE b3536 0.62 -1.6 3,695,462 - bcsE b3536 0.62 -0.6 3,695,462 - bcsE b3536 0.62 +1.9 3,695,472 + bcsE b3536 0.63 -0.2 3,695,480 - bcsE b3536 0.64 -2.8 3,695,480 - bcsE b3536 0.64 -0.8 3,695,520 + bcsE b3536 0.66 +0.2 3,695,567 - bcsE b3536 0.69 -2.6 3,695,567 - bcsE b3536 0.69 -0.1 3,695,581 + bcsE b3536 0.70 +1.4 3,695,582 - bcsE b3536 0.70 +1.1 3,695,583 + bcsE b3536 0.70 -0.5 3,695,591 + bcsE b3536 0.71 +0.4 3,695,596 + bcsE b3536 0.71 +1.8 3,695,632 + bcsE b3536 0.73 -3.9 3,695,637 - bcsE b3536 0.74 +2.1 3,695,637 - bcsE b3536 0.74 +2.8 3,695,638 - bcsE b3536 0.74 +0.2 3,695,644 + bcsE b3536 0.74 +1.4 3,695,644 + bcsE b3536 0.74 -1.0 3,695,700 + bcsE b3536 0.78 +2.6 3,695,757 + bcsE b3536 0.81 +1.4 3,695,814 + bcsE b3536 0.85 -0.7 3,695,871 - bcsE b3536 0.88 +0.1 3,695,894 + bcsE b3536 0.90 +0.9 3,695,902 - -2.2 3,695,902 - +0.5 3,695,902 - -0.0 3,695,926 + +1.9 3,695,928 - +0.6 3,695,938 + +0.9 3,695,952 + +0.5 3,695,952 + +1.6 3,695,965 + +0.5 3,695,985 + -0.9 3,695,994 + +1.9 3,696,022 + +0.2 3,696,022 + -2.0 3,696,032 + -1.0 3,696,124 - yhjT b3537 0.39 +0.2 3,696,124 - yhjT b3537 0.39 +2.1 3,696,131 + yhjT b3537 0.43 +1.4 3,696,131 + yhjT b3537 0.43 +0.3 3,696,199 + yhjT b3537 0.78 +0.5 3,696,205 - yhjT b3537 0.81 -0.6 3,696,268 + +0.8 3,696,292 - -2.1 3,696,338 + -1.1 3,696,338 + -1.4 3,696,346 - +2.5 3,696,346 - -0.2 3,696,352 + -0.8 3,696,416 + bcsG b3538 0.11 -1.4 3,696,416 + bcsG b3538 0.11 +1.8 3,696,416 + bcsG b3538 0.11 -1.0 3,696,431 - bcsG b3538 0.12 +0.9 3,696,445 - bcsG b3538 0.12 -0.6 3,696,450 - bcsG b3538 0.13 +0.4 3,696,473 - bcsG b3538 0.14 -0.2 3,696,473 - bcsG b3538 0.14 +1.8 3,696,473 - bcsG b3538 0.14 -1.2 3,696,504 - bcsG b3538 0.16 -0.2 3,696,519 + bcsG b3538 0.17 +0.8 3,696,544 - bcsG b3538 0.18 +0.8 3,696,581 - bcsG b3538 0.20 +0.2 3,696,630 - bcsG b3538 0.23 +0.8 3,696,677 - bcsG b3538 0.26 -1.4 3,696,677 - bcsG b3538 0.26 -1.0 3,696,681 - bcsG b3538 0.26 +1.2 3,696,714 + bcsG b3538 0.28 +0.4 3,696,753 - bcsG b3538 0.31 -1.3 3,696,753 - bcsG b3538 0.31 +1.2 3,696,771 - bcsG b3538 0.32 -0.4 3,696,771 - bcsG b3538 0.32 +2.0 3,696,804 + bcsG b3538 0.34 +0.8 3,696,804 + bcsG b3538 0.34 -0.8 3,696,829 - bcsG b3538 0.35 +2.4 3,696,833 + bcsG b3538 0.35 -0.4 3,696,833 + bcsG b3538 0.35 +0.9 3,696,851 + bcsG b3538 0.37 +0.7 3,696,925 + bcsG b3538 0.41 -0.5 3,696,950 + bcsG b3538 0.42 +2.2 3,696,980 + +0.2 3,696,989 - bcsG b3538 0.45 -0.2 3,697,005 - bcsG b3538 0.46 -2.2 3,697,005 - bcsG b3538 0.46 -1.9 3,697,052 + bcsG b3538 0.49 +0.8 3,697,061 + bcsG b3538 0.49 +0.0 3,697,062 + bcsG b3538 0.49 -0.2 3,697,067 - bcsG b3538 0.49 +1.4 3,697,069 - bcsG b3538 0.50 -0.0 3,697,072 + bcsG b3538 0.50 +1.4 3,697,084 + bcsG b3538 0.50 +2.1 3,697,116 + bcsG b3538 0.52 +0.8 3,697,120 + bcsG b3538 0.53 +1.8 3,697,128 - bcsG b3538 0.53 -1.6 3,697,138 - bcsG b3538 0.54 +3.2 3,697,326 + bcsG b3538 0.65 -2.8 3,697,336 + bcsG b3538 0.65 +2.4 3,697,339 + bcsG b3538 0.66 -1.6 3,697,339 + bcsG b3538 0.66 -0.8 3,697,344 + bcsG b3538 0.66 -1.8 3,697,352 - bcsG b3538 0.66 +1.0 3,697,395 - bcsG b3538 0.69 -0.8 3,697,395 - bcsG b3538 0.69 +3.6 3,697,401 - bcsG b3538 0.69 -0.7 3,697,401 - bcsG b3538 0.69 -1.2 3,697,408 + bcsG b3538 0.70 +0.6 3,697,419 - bcsG b3538 0.70 -1.9 3,697,433 - bcsG b3538 0.71 -0.2 3,697,441 - bcsG b3538 0.72 +0.3 3,697,441 - bcsG b3538 0.72 +2.4 3,697,547 - bcsG b3538 0.78 +1.2 3,697,560 + bcsG b3538 0.79 +2.5 3,697,568 - bcsG b3538 0.79 +0.5 3,697,570 - bcsG b3538 0.79 -1.5 3,697,607 + bcsG b3538 0.82 +0.5 3,697,607 + bcsG b3538 0.82 -1.8 3,697,615 + bcsG b3538 0.82 -1.5 3,697,615 + bcsG b3538 0.82 -0.2 3,697,677 + bcsG b3538 0.86 +0.0 3,697,736 + bcsG b3538 0.89 +0.8 3,697,861 + +1.5 3,697,886 - -1.4 3,697,886 - +1.8 3,697,945 + +0.2 3,697,945 + -1.2 3,698,018 + ldrD b4453 0.14 +1.6 3,698,052 + ldrD b4453 0.45 -1.0 3,698,055 - ldrD b4453 0.48 -0.8 3,698,103 - -1.5 3,698,103 - -0.5 3,698,107 + -0.4 3,698,158 + -1.7 3,698,158 + -0.3 3,698,158 + +0.6 3,698,158 + +1.3 3,698,165 + +0.5 3,698,165 + +1.7 3,698,166 - rdlD b4454 0.11 -1.0 3,698,178 - rdlD b4454 0.29 -2.5 3,698,185 + rdlD b4454 0.39 +0.7 3,698,190 + rdlD b4454 0.47 -0.3 3,698,195 + rdlD b4454 0.55 -1.4 3,698,195 + rdlD b4454 0.55 +0.6 3,698,195 + rdlD b4454 0.55 -0.8 3,698,195 + rdlD b4454 0.55 +0.8 3,698,257 - +0.5 3,698,260 + +2.2 3,698,277 + +2.5 3,698,318 + -1.7 3,698,333 + -0.1 3,698,333 + -1.8 3,698,340 + +3.0 3,698,407 - -0.8 3,698,410 + -2.3 3,698,410 + +2.2 3,698,410 + +2.1 3,698,410 + -0.4 3,698,411 + +0.9 3,698,418 - +0.8 3,698,418 - +0.0 3,698,418 - +0.0 3,698,429 - +0.8 3,698,429 - -0.5 3,698,429 - -1.0 3,698,452 + +1.6 3,698,452 - -0.2 3,698,473 + -1.5 3,698,474 - +0.2 3,698,474 - +0.6 3,698,487 + +1.6 3,698,487 + +0.8 3,698,501 + +3.0 3,698,501 + -0.5 3,698,503 + +1.0 3,698,503 + -1.8 3,698,507 - -0.4 3,698,519 - +1.8 3,698,533 - -0.2 3,698,533 - +0.8 3,698,533 - -1.5 3,698,589 - +1.9 3,698,591 + +0.5 3,698,591 + -0.5 3,698,593 + -1.2 3,698,607 - +1.6 3,698,612 - -0.2 3,698,632 + -1.8 3,698,632 + +1.2 3,698,634 + +1.6 3,698,640 + -0.4 3,698,642 - -0.2 3,698,660 + -1.8 3,698,660 + -0.5 3,698,667 + -0.7 3,698,681 + +1.1 3,698,681 + +1.6 3,698,683 + -2.3 3,698,683 + -0.5 3,698,689 + +0.4 3,698,690 - +1.6 3,698,691 + +0.8 3,698,691 + -1.5 3,698,693 + -1.2 3,698,694 - +0.7 3,698,696 - -0.0 3,698,696 - +1.0 3,698,699 - -0.2 3,698,699 - -0.5 3,698,699 - -1.5 3,698,701 - +0.5 3,698,715 - yhjV b3539 0.10 +1.0 3,698,755 - yhjV b3539 0.13 -0.9 3,698,760 + yhjV b3539 0.14 +0.8 3,698,760 - yhjV b3539 0.14 -2.8 3,698,760 - yhjV b3539 0.14 -0.1 3,698,761 - yhjV b3539 0.14 +0.8 3,698,761 - yhjV b3539 0.14 +1.4 3,698,765 + yhjV b3539 0.14 +0.2 3,698,765 + yhjV b3539 0.14 +1.7 3,698,765 + yhjV b3539 0.14 +0.7 3,698,765 + yhjV b3539 0.14 -1.4 3,698,765 + yhjV b3539 0.14 -1.9 3,698,773 - yhjV b3539 0.15 +1.8 3,698,773 - yhjV b3539 0.15 -1.3 3,698,773 - yhjV b3539 0.15 +0.6 3,698,773 - yhjV b3539 0.15 -1.5 3,698,775 + yhjV b3539 0.15 +1.0 3,698,783 + yhjV b3539 0.15 +1.3 3,698,783 - yhjV b3539 0.15 +0.2 3,698,783 - yhjV b3539 0.15 +0.5 3,698,783 - yhjV b3539 0.15 +2.4 3,698,822 + yhjV b3539 0.19 -1.0 3,698,847 + yhjV b3539 0.21 -2.1 3,698,850 - yhjV b3539 0.21 -2.2 3,698,850 - yhjV b3539 0.21 +1.8 3,698,856 - yhjV b3539 0.21 -1.5 3,698,909 - yhjV b3539 0.25 +2.6 3,698,909 - yhjV b3539 0.25 -0.0
Or see this region's nucleotide sequence