Strain Fitness in Escherichia coli BW25113 around b3057

Experiment: Benzalkonium Chloride 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcca and bacA are separated by 180 nucleotidesbacA and ygiG are separated by 89 nucleotidesygiG and ygiH are separated by 104 nucleotides b3056: cca - fused tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase and phosphatase (NCBI), at 3,199,913 to 3,201,151 cca b3057: bacA - undecaprenyl pyrophosphate phosphatase (NCBI), at 3,201,332 to 3,202,153 bacA b3058: ygiG - putative kinase (VIMSS), at 3,202,243 to 3,202,611 ygiG b3059: ygiH - hypothetical protein (NCBI), at 3,202,716 to 3,203,333 ygiH Position (kb) 3201 3202 3203Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3201.212 kb on + strandat 3201.294 kb on + strandat 3201.343 kb on - strandat 3201.352 kb on - strandat 3201.439 kb on + strand, within bacAat 3201.489 kb on - strand, within bacAat 3201.489 kb on - strand, within bacAat 3201.512 kb on + strand, within bacAat 3201.512 kb on + strand, within bacAat 3201.537 kb on - strand, within bacAat 3201.537 kb on - strand, within bacAat 3201.640 kb on + strand, within bacAat 3201.640 kb on + strand, within bacAat 3201.696 kb on + strand, within bacAat 3201.707 kb on - strand, within bacAat 3201.707 kb on - strand, within bacAat 3201.765 kb on - strand, within bacAat 3201.880 kb on + strand, within bacAat 3201.970 kb on - strand, within bacAat 3201.978 kb on - strand, within bacAat 3201.995 kb on - strand, within bacAat 3202.065 kb on - strand, within bacAat 3202.069 kb on - strand, within bacAat 3202.069 kb on - strand, within bacAat 3202.081 kb on - strandat 3202.081 kb on - strandat 3202.136 kb on + strandat 3202.136 kb on + strandat 3202.139 kb on + strandat 3202.187 kb on + strandat 3202.219 kb on - strandat 3202.602 kb on - strandat 3202.644 kb on - strandat 3202.712 kb on + strandat 3202.878 kb on + strand, within ygiHat 3202.878 kb on + strand, within ygiHat 3202.968 kb on - strand, within ygiHat 3203.057 kb on + strand, within ygiHat 3203.072 kb on - strand, within ygiHat 3203.128 kb on - strand, within ygiHat 3203.143 kb on + strand, within ygiH

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml
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3,201,212 + +0.4
3,201,294 + -2.2
3,201,343 - +1.0
3,201,352 - +0.5
3,201,439 + bacA b3057 0.13 +1.5
3,201,489 - bacA b3057 0.19 +2.3
3,201,489 - bacA b3057 0.19 -1.2
3,201,512 + bacA b3057 0.22 +2.2
3,201,512 + bacA b3057 0.22 +2.4
3,201,537 - bacA b3057 0.25 -2.1
3,201,537 - bacA b3057 0.25 +0.1
3,201,640 + bacA b3057 0.37 +1.1
3,201,640 + bacA b3057 0.37 -1.2
3,201,696 + bacA b3057 0.44 -0.9
3,201,707 - bacA b3057 0.46 -2.5
3,201,707 - bacA b3057 0.46 +0.2
3,201,765 - bacA b3057 0.53 -1.8
3,201,880 + bacA b3057 0.67 +3.3
3,201,970 - bacA b3057 0.78 +2.1
3,201,978 - bacA b3057 0.79 +0.4
3,201,995 - bacA b3057 0.81 +2.1
3,202,065 - bacA b3057 0.89 +0.4
3,202,069 - bacA b3057 0.90 -0.8
3,202,069 - bacA b3057 0.90 -0.6
3,202,081 - -0.2
3,202,081 - -1.4
3,202,136 + +0.5
3,202,136 + +2.6
3,202,139 + +1.4
3,202,187 + -2.2
3,202,219 - -1.8
3,202,602 - +1.1
3,202,644 - -0.5
3,202,712 + -0.4
3,202,878 + ygiH b3059 0.26 +0.7
3,202,878 + ygiH b3059 0.26 +2.6
3,202,968 - ygiH b3059 0.41 -0.4
3,203,057 + ygiH b3059 0.55 -1.4
3,203,072 - ygiH b3059 0.58 -1.4
3,203,128 - ygiH b3059 0.67 +1.0
3,203,143 + ygiH b3059 0.69 -0.8

Or see this region's nucleotide sequence