Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tas and ygeD are separated by 31 nucleotides ygeD and aas overlap by 8 nucleotides
b2834: tas - predicted oxidoreductase, NADP(H)-dependent aldo-keto reductase (NCBI), at 2,969,619 to 2,970,659
tas
b2835: ygeD - predicted inner membrane protein (NCBI), at 2,970,691 to 2,971,884
ygeD
b2836: aas - 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI), at 2,971,877 to 2,974,036
aas
Position (kb)
2970
2971
2972 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2969.693 kb on + strand at 2969.699 kb on - strand at 2969.746 kb on + strand, within tas at 2969.746 kb on + strand, within tas at 2969.753 kb on - strand, within tas at 2969.755 kb on - strand, within tas at 2969.872 kb on + strand, within tas at 2969.872 kb on + strand, within tas at 2969.912 kb on - strand, within tas at 2969.917 kb on + strand, within tas at 2969.976 kb on - strand, within tas at 2969.976 kb on - strand, within tas at 2969.984 kb on - strand, within tas at 2969.993 kb on + strand, within tas at 2970.254 kb on - strand, within tas at 2970.254 kb on - strand, within tas at 2970.283 kb on - strand, within tas at 2970.290 kb on + strand, within tas at 2970.324 kb on - strand, within tas at 2970.336 kb on + strand, within tas at 2970.336 kb on + strand, within tas at 2970.410 kb on + strand, within tas at 2970.471 kb on + strand, within tas at 2970.471 kb on + strand, within tas at 2970.480 kb on - strand, within tas at 2970.499 kb on + strand, within tas at 2970.499 kb on + strand, within tas at 2970.601 kb on - strand at 2970.635 kb on + strand at 2970.635 kb on + strand at 2970.654 kb on + strand at 2970.654 kb on + strand at 2970.654 kb on + strand at 2970.710 kb on + strand at 2970.781 kb on - strand at 2970.781 kb on - strand at 2970.900 kb on - strand, within ygeD at 2970.970 kb on - strand, within ygeD at 2971.028 kb on + strand, within ygeD at 2971.028 kb on + strand, within ygeD at 2971.031 kb on - strand, within ygeD at 2971.031 kb on - strand, within ygeD at 2971.033 kb on - strand, within ygeD at 2971.068 kb on - strand, within ygeD at 2971.154 kb on - strand, within ygeD at 2971.180 kb on + strand, within ygeD at 2971.303 kb on - strand, within ygeD at 2971.305 kb on + strand, within ygeD at 2971.360 kb on + strand, within ygeD at 2971.360 kb on + strand, within ygeD at 2971.438 kb on - strand, within ygeD at 2971.489 kb on + strand, within ygeD at 2971.489 kb on + strand, within ygeD at 2971.489 kb on + strand, within ygeD at 2971.571 kb on - strand, within ygeD at 2971.581 kb on + strand, within ygeD at 2971.617 kb on + strand, within ygeD at 2971.625 kb on - strand, within ygeD at 2971.625 kb on - strand, within ygeD at 2971.634 kb on + strand, within ygeD at 2971.656 kb on - strand, within ygeD at 2971.656 kb on - strand, within ygeD at 2971.737 kb on - strand, within ygeD at 2971.737 kb on - strand, within ygeD at 2971.753 kb on - strand, within ygeD at 2971.776 kb on - strand at 2971.857 kb on + strand at 2971.864 kb on + strand at 2971.869 kb on + strand at 2971.869 kb on + strand at 2971.970 kb on + strand at 2972.106 kb on - strand, within aas at 2972.202 kb on - strand, within aas at 2972.210 kb on + strand, within aas at 2972.215 kb on + strand, within aas at 2972.215 kb on + strand, within aas at 2972.239 kb on + strand, within aas at 2972.261 kb on - strand, within aas at 2972.261 kb on - strand, within aas at 2972.467 kb on + strand, within aas at 2972.498 kb on - strand, within aas at 2972.589 kb on - strand, within aas at 2972.629 kb on + strand, within aas at 2972.660 kb on - strand, within aas at 2972.660 kb on - strand, within aas at 2972.667 kb on + strand, within aas at 2972.668 kb on - strand, within aas at 2972.750 kb on - strand, within aas at 2972.750 kb on - strand, within aas at 2972.752 kb on - strand, within aas at 2972.752 kb on - strand, within aas at 2972.776 kb on + strand, within aas at 2972.789 kb on + strand, within aas
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 2,969,693 + -1.0 2,969,699 - +1.7 2,969,746 + tas b2834 0.12 -0.2 2,969,746 + tas b2834 0.12 +0.3 2,969,753 - tas b2834 0.13 -1.4 2,969,755 - tas b2834 0.13 -0.4 2,969,872 + tas b2834 0.24 +1.5 2,969,872 + tas b2834 0.24 +0.2 2,969,912 - tas b2834 0.28 +0.8 2,969,917 + tas b2834 0.29 +2.5 2,969,976 - tas b2834 0.34 -1.0 2,969,976 - tas b2834 0.34 -0.2 2,969,984 - tas b2834 0.35 -1.8 2,969,993 + tas b2834 0.36 -0.7 2,970,254 - tas b2834 0.61 -0.7 2,970,254 - tas b2834 0.61 -1.2 2,970,283 - tas b2834 0.64 +1.2 2,970,290 + tas b2834 0.64 -0.3 2,970,324 - tas b2834 0.68 -1.1 2,970,336 + tas b2834 0.69 -1.2 2,970,336 + tas b2834 0.69 -1.0 2,970,410 + tas b2834 0.76 -1.2 2,970,471 + tas b2834 0.82 +1.3 2,970,471 + tas b2834 0.82 +0.5 2,970,480 - tas b2834 0.83 -2.2 2,970,499 + tas b2834 0.85 -1.5 2,970,499 + tas b2834 0.85 -1.0 2,970,601 - +1.3 2,970,635 + +0.1 2,970,635 + +3.2 2,970,654 + -1.5 2,970,654 + -0.2 2,970,654 + +0.5 2,970,710 + -2.4 2,970,781 - -0.2 2,970,781 - -1.2 2,970,900 - ygeD b2835 0.18 +2.6 2,970,970 - ygeD b2835 0.23 +1.0 2,971,028 + ygeD b2835 0.28 +0.9 2,971,028 + ygeD b2835 0.28 +1.3 2,971,031 - ygeD b2835 0.28 -0.2 2,971,031 - ygeD b2835 0.28 -1.8 2,971,033 - ygeD b2835 0.29 -0.4 2,971,068 - ygeD b2835 0.32 +1.8 2,971,154 - ygeD b2835 0.39 +0.8 2,971,180 + ygeD b2835 0.41 +1.2 2,971,303 - ygeD b2835 0.51 +0.2 2,971,305 + ygeD b2835 0.51 +1.0 2,971,360 + ygeD b2835 0.56 +0.5 2,971,360 + ygeD b2835 0.56 -2.1 2,971,438 - ygeD b2835 0.63 +0.3 2,971,489 + ygeD b2835 0.67 +1.3 2,971,489 + ygeD b2835 0.67 +2.9 2,971,489 + ygeD b2835 0.67 +2.2 2,971,571 - ygeD b2835 0.74 +1.1 2,971,581 + ygeD b2835 0.75 +0.1 2,971,617 + ygeD b2835 0.78 +2.9 2,971,625 - ygeD b2835 0.78 -1.0 2,971,625 - ygeD b2835 0.78 +0.8 2,971,634 + ygeD b2835 0.79 -2.5 2,971,656 - ygeD b2835 0.81 -1.5 2,971,656 - ygeD b2835 0.81 +1.3 2,971,737 - ygeD b2835 0.88 +1.8 2,971,737 - ygeD b2835 0.88 +0.3 2,971,753 - ygeD b2835 0.89 +1.7 2,971,776 - +0.8 2,971,857 + +0.2 2,971,864 + +1.3 2,971,869 + -0.6 2,971,869 + -1.7 2,971,970 + +2.6 2,972,106 - aas b2836 0.11 +1.8 2,972,202 - aas b2836 0.15 +1.2 2,972,210 + aas b2836 0.15 +1.5 2,972,215 + aas b2836 0.16 +3.2 2,972,215 + aas b2836 0.16 +2.8 2,972,239 + aas b2836 0.17 -0.1 2,972,261 - aas b2836 0.18 +1.3 2,972,261 - aas b2836 0.18 -0.7 2,972,467 + aas b2836 0.27 -0.8 2,972,498 - aas b2836 0.29 +2.1 2,972,589 - aas b2836 0.33 -0.2 2,972,629 + aas b2836 0.35 -0.6 2,972,660 - aas b2836 0.36 +1.8 2,972,660 - aas b2836 0.36 +1.3 2,972,667 + aas b2836 0.37 +1.8 2,972,668 - aas b2836 0.37 -0.5 2,972,750 - aas b2836 0.40 -1.2 2,972,750 - aas b2836 0.40 +0.6 2,972,752 - aas b2836 0.41 +1.6 2,972,752 - aas b2836 0.41 +1.9 2,972,776 + aas b2836 0.42 +2.6 2,972,789 + aas b2836 0.42 +0.8
Or see this region's nucleotide sequence