Strain Fitness in Escherichia coli BW25113 around b1866

Experiment: Benzalkonium Chloride 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyebC and nudB are separated by 28 nucleotidesnudB and aspS are separated by 117 nucleotidesaspS and yecD are separated by 309 nucleotidesyecD and yecE overlap by 4 nucleotides b1864: yebC - hypothetical protein (NCBI), at 1,945,435 to 1,946,175 yebC b1865: nudB - dATP pyrophosphohydrolase (NCBI), at 1,946,204 to 1,946,656 nudB b1866: aspS - aspartyl-tRNA synthetase (NCBI), at 1,946,774 to 1,948,546 aspS b1867: yecD - orf, hypothetical protein (VIMSS), at 1,948,856 to 1,949,422 yecD b1868: yecE - hypothetical protein (NCBI), at 1,949,419 to 1,950,237 yecE Position (kb) 1946 1947 1948 1949Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 1945.975 kb on + strand, within yebCat 1945.975 kb on + strand, within yebCat 1945.997 kb on + strand, within yebCat 1946.123 kb on - strandat 1946.247 kb on + strandat 1946.247 kb on + strandat 1946.435 kb on + strand, within nudBat 1946.471 kb on - strand, within nudBat 1946.471 kb on - strand, within nudBat 1946.636 kb on + strandat 1946.644 kb on - strandat 1948.556 kb on - strandat 1948.718 kb on - strandat 1948.761 kb on + strandat 1948.771 kb on + strandat 1948.859 kb on - strandat 1948.859 kb on - strandat 1948.932 kb on + strand, within yecDat 1949.048 kb on + strand, within yecDat 1949.173 kb on + strand, within yecDat 1949.173 kb on + strand, within yecDat 1949.179 kb on - strand, within yecDat 1949.186 kb on + strand, within yecDat 1949.186 kb on + strand, within yecDat 1949.189 kb on - strand, within yecDat 1949.228 kb on + strand, within yecDat 1949.228 kb on + strand, within yecDat 1949.228 kb on - strand, within yecDat 1949.228 kb on - strand, within yecDat 1949.231 kb on - strand, within yecDat 1949.349 kb on + strand, within yecDat 1949.404 kb on - strandat 1949.404 kb on - strandat 1949.442 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml
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1,945,975 + yebC b1864 0.73 +2.2
1,945,975 + yebC b1864 0.73 +1.1
1,945,997 + yebC b1864 0.76 +1.3
1,946,123 - +1.1
1,946,247 + +6.4
1,946,247 + +6.1
1,946,435 + nudB b1865 0.51 +1.1
1,946,471 - nudB b1865 0.59 +6.3
1,946,471 - nudB b1865 0.59 +6.4
1,946,636 + +0.4
1,946,644 - +2.9
1,948,556 - -1.9
1,948,718 - +0.3
1,948,761 + +1.1
1,948,771 + +1.9
1,948,859 - +0.1
1,948,859 - +0.1
1,948,932 + yecD b1867 0.13 -0.0
1,949,048 + yecD b1867 0.34 +0.4
1,949,173 + yecD b1867 0.56 -1.7
1,949,173 + yecD b1867 0.56 +0.4
1,949,179 - yecD b1867 0.57 +1.9
1,949,186 + yecD b1867 0.58 -1.4
1,949,186 + yecD b1867 0.58 -1.7
1,949,189 - yecD b1867 0.59 -0.5
1,949,228 + yecD b1867 0.66 -0.3
1,949,228 + yecD b1867 0.66 +0.7
1,949,228 - yecD b1867 0.66 +1.8
1,949,228 - yecD b1867 0.66 +0.6
1,949,231 - yecD b1867 0.66 +1.0
1,949,349 + yecD b1867 0.87 +0.1
1,949,404 - +1.1
1,949,404 - -1.2
1,949,442 - -2.8

Or see this region's nucleotide sequence