Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydgI and folM are separated by 36 nucleotides folM and ydgC overlap by 4 nucleotides ydgC and rstA are separated by 128 nucleotides
b1605: ydgI - predicted arginine/ornithine antiporter transporter (NCBI), at 1,677,581 to 1,678,963
ydgI
b1606: folM - short chain dehydrogenase (NCBI), at 1,679,000 to 1,679,722
folM
b1607: ydgC - conserved inner membrane protein associated with alginate biosynthesis (NCBI), at 1,679,719 to 1,680,054
ydgC
b1608: rstA - DNA-binding response regulator in two-component regulatory system with RstB (RefSeq), at 1,680,183 to 1,680,902
rstA
Position (kb)
1678
1679
1680 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1678.025 kb on + strand, within ydgI at 1678.025 kb on + strand, within ydgI at 1678.025 kb on + strand, within ydgI at 1678.148 kb on + strand, within ydgI at 1678.154 kb on - strand, within ydgI at 1678.295 kb on - strand, within ydgI at 1678.353 kb on + strand, within ydgI at 1678.353 kb on + strand, within ydgI at 1678.422 kb on - strand, within ydgI at 1678.477 kb on - strand, within ydgI at 1678.873 kb on - strand at 1678.883 kb on + strand at 1678.883 kb on + strand at 1678.919 kb on + strand at 1679.076 kb on + strand, within folM at 1679.076 kb on + strand, within folM at 1679.126 kb on + strand, within folM at 1679.126 kb on + strand, within folM at 1679.165 kb on - strand, within folM at 1679.165 kb on - strand, within folM at 1679.198 kb on - strand, within folM at 1679.226 kb on + strand, within folM at 1679.328 kb on - strand, within folM at 1679.336 kb on - strand, within folM at 1679.342 kb on + strand, within folM at 1679.342 kb on - strand, within folM at 1679.342 kb on - strand, within folM at 1679.342 kb on - strand, within folM at 1679.349 kb on + strand, within folM at 1679.376 kb on + strand, within folM at 1679.390 kb on + strand, within folM at 1679.484 kb on - strand, within folM at 1679.538 kb on + strand, within folM at 1679.538 kb on + strand, within folM at 1679.887 kb on + strand, within ydgC at 1679.897 kb on + strand, within ydgC at 1679.924 kb on - strand, within ydgC at 1680.147 kb on + strand at 1680.268 kb on + strand, within rstA at 1680.438 kb on + strand, within rstA at 1680.439 kb on + strand, within rstA at 1680.439 kb on + strand, within rstA at 1680.544 kb on + strand, within rstA at 1680.552 kb on - strand, within rstA at 1680.552 kb on - strand, within rstA at 1680.575 kb on + strand, within rstA
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 1,678,025 + ydgI b1605 0.32 -0.8 1,678,025 + ydgI b1605 0.32 -2.3 1,678,025 + ydgI b1605 0.32 -0.6 1,678,148 + ydgI b1605 0.41 -1.4 1,678,154 - ydgI b1605 0.41 -0.4 1,678,295 - ydgI b1605 0.52 +0.9 1,678,353 + ydgI b1605 0.56 -1.6 1,678,353 + ydgI b1605 0.56 -0.4 1,678,422 - ydgI b1605 0.61 -2.0 1,678,477 - ydgI b1605 0.65 -0.4 1,678,873 - +0.3 1,678,883 + -0.6 1,678,883 + +1.3 1,678,919 + +0.5 1,679,076 + folM b1606 0.11 -1.6 1,679,076 + folM b1606 0.11 +1.2 1,679,126 + folM b1606 0.17 +1.0 1,679,126 + folM b1606 0.17 -1.4 1,679,165 - folM b1606 0.23 +1.9 1,679,165 - folM b1606 0.23 +0.6 1,679,198 - folM b1606 0.27 +0.6 1,679,226 + folM b1606 0.31 -2.6 1,679,328 - folM b1606 0.45 +1.3 1,679,336 - folM b1606 0.46 -1.0 1,679,342 + folM b1606 0.47 +1.6 1,679,342 - folM b1606 0.47 +1.5 1,679,342 - folM b1606 0.47 +0.2 1,679,342 - folM b1606 0.47 +2.0 1,679,349 + folM b1606 0.48 -0.0 1,679,376 + folM b1606 0.52 +0.3 1,679,390 + folM b1606 0.54 +1.0 1,679,484 - folM b1606 0.67 +1.0 1,679,538 + folM b1606 0.74 +1.0 1,679,538 + folM b1606 0.74 -0.6 1,679,887 + ydgC b1607 0.50 +0.6 1,679,897 + ydgC b1607 0.53 -0.8 1,679,924 - ydgC b1607 0.61 -1.0 1,680,147 + -0.8 1,680,268 + rstA b1608 0.12 +1.8 1,680,438 + rstA b1608 0.35 +3.3 1,680,439 + rstA b1608 0.36 +1.3 1,680,439 + rstA b1608 0.36 +0.3 1,680,544 + rstA b1608 0.50 +0.2 1,680,552 - rstA b1608 0.51 -0.6 1,680,552 - rstA b1608 0.51 +0.1 1,680,575 + rstA b1608 0.54 -0.3
Or see this region's nucleotide sequence