Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydcZ and yncA overlap by 4 nucleotides yncA and yncB are separated by 180 nucleotides yncB and b1450 are separated by 197 nucleotides b1450 and yncD are separated by 35 nucleotides
b1447: ydcZ - predicted inner membrane protein (NCBI), at 1,515,906 to 1,516,355
ydcZ
b1448: yncA - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 1,516,352 to 1,516,870
yncA
b1449: yncB - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (RefSeq), at 1,517,051 to 1,518,088
yncB
b1450: b1450 - orf, hypothetical protein (VIMSS), at 1,518,286 to 1,518,951
b1450
b1451: yncD - predicted iron outer membrane transporter (NCBI), at 1,518,987 to 1,521,089
yncD
Position (kb)
1517
1518
1519 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1516.613 kb on - strand, within yncA at 1516.789 kb on - strand, within yncA at 1516.831 kb on + strand at 1516.870 kb on + strand at 1516.878 kb on - strand at 1517.165 kb on + strand, within yncB at 1517.165 kb on + strand, within yncB at 1517.246 kb on - strand, within yncB at 1517.310 kb on + strand, within yncB at 1517.341 kb on + strand, within yncB at 1517.341 kb on + strand, within yncB at 1517.496 kb on - strand, within yncB at 1517.517 kb on - strand, within yncB at 1517.635 kb on - strand, within yncB at 1517.635 kb on - strand, within yncB at 1517.739 kb on - strand, within yncB at 1517.739 kb on - strand, within yncB at 1517.741 kb on - strand, within yncB at 1517.805 kb on + strand, within yncB at 1517.805 kb on + strand, within yncB at 1517.839 kb on - strand, within yncB at 1517.839 kb on - strand, within yncB at 1517.923 kb on + strand, within yncB at 1517.923 kb on + strand, within yncB at 1517.950 kb on - strand, within yncB at 1517.950 kb on - strand, within yncB at 1518.047 kb on - strand at 1518.065 kb on - strand at 1518.147 kb on + strand at 1518.175 kb on - strand at 1518.175 kb on - strand at 1518.200 kb on + strand at 1518.225 kb on - strand at 1518.237 kb on + strand at 1518.254 kb on + strand at 1518.295 kb on - strand at 1518.330 kb on + strand at 1518.338 kb on - strand at 1518.349 kb on - strand at 1518.354 kb on + strand, within b1450 at 1518.423 kb on + strand, within b1450 at 1518.425 kb on + strand, within b1450 at 1518.425 kb on + strand, within b1450 at 1518.425 kb on + strand, within b1450 at 1518.425 kb on + strand, within b1450 at 1518.495 kb on + strand, within b1450 at 1518.498 kb on + strand, within b1450 at 1518.506 kb on + strand, within b1450 at 1518.746 kb on - strand, within b1450 at 1518.756 kb on + strand, within b1450 at 1518.756 kb on + strand, within b1450 at 1518.767 kb on + strand, within b1450 at 1518.775 kb on - strand, within b1450 at 1518.779 kb on + strand, within b1450 at 1518.844 kb on + strand, within b1450 at 1518.844 kb on + strand, within b1450 at 1518.871 kb on + strand, within b1450 at 1518.872 kb on + strand, within b1450 at 1518.954 kb on - strand at 1518.974 kb on + strand at 1519.053 kb on - strand at 1519.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 1,516,613 - yncA b1448 0.50 +0.5 1,516,789 - yncA b1448 0.84 -0.1 1,516,831 + +0.4 1,516,870 + +0.2 1,516,878 - +0.4 1,517,165 + yncB b1449 0.11 -0.5 1,517,165 + yncB b1449 0.11 -1.9 1,517,246 - yncB b1449 0.19 +0.6 1,517,310 + yncB b1449 0.25 -1.2 1,517,341 + yncB b1449 0.28 +0.5 1,517,341 + yncB b1449 0.28 +0.8 1,517,496 - yncB b1449 0.43 +0.1 1,517,517 - yncB b1449 0.45 -0.6 1,517,635 - yncB b1449 0.56 -1.9 1,517,635 - yncB b1449 0.56 -1.2 1,517,739 - yncB b1449 0.66 -2.2 1,517,739 - yncB b1449 0.66 -0.2 1,517,741 - yncB b1449 0.66 +0.1 1,517,805 + yncB b1449 0.73 -2.1 1,517,805 + yncB b1449 0.73 +0.9 1,517,839 - yncB b1449 0.76 +1.7 1,517,839 - yncB b1449 0.76 -1.2 1,517,923 + yncB b1449 0.84 -1.2 1,517,923 + yncB b1449 0.84 +0.1 1,517,950 - yncB b1449 0.87 -0.1 1,517,950 - yncB b1449 0.87 +2.2 1,518,047 - -0.7 1,518,065 - -2.1 1,518,147 + -1.2 1,518,175 - -0.9 1,518,175 - -1.7 1,518,200 + +0.6 1,518,225 - +1.1 1,518,237 + +0.6 1,518,254 + +0.2 1,518,295 - +0.2 1,518,330 + +1.2 1,518,338 - -0.1 1,518,349 - -0.5 1,518,354 + b1450 b1450 0.10 +1.3 1,518,423 + b1450 b1450 0.21 +1.1 1,518,425 + b1450 b1450 0.21 +2.7 1,518,425 + b1450 b1450 0.21 +0.9 1,518,425 + b1450 b1450 0.21 +2.1 1,518,425 + b1450 b1450 0.21 +0.1 1,518,495 + b1450 b1450 0.31 +1.5 1,518,498 + b1450 b1450 0.32 -1.5 1,518,506 + b1450 b1450 0.33 +0.1 1,518,746 - b1450 b1450 0.69 -2.1 1,518,756 + b1450 b1450 0.71 +2.4 1,518,756 + b1450 b1450 0.71 -2.4 1,518,767 + b1450 b1450 0.72 +1.7 1,518,775 - b1450 b1450 0.73 -1.5 1,518,779 + b1450 b1450 0.74 +2.0 1,518,844 + b1450 b1450 0.84 +0.1 1,518,844 + b1450 b1450 0.84 +1.6 1,518,871 + b1450 b1450 0.88 -1.9 1,518,872 + b1450 b1450 0.88 +0.1 1,518,954 - -0.4 1,518,974 + +1.1 1,519,053 - -1.5 1,519,084 - +0.1
Or see this region's nucleotide sequence