Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mviN and flgN are separated by 39 nucleotides flgN and flgM are separated by 4 nucleotides flgM and flgA are separated by 75 nucleotides flgA and flgB are separated by 154 nucleotides flgB and flgC are separated by 3 nucleotides flgC and flgD are separated by 11 nucleotides
b1069: mviN - predicted inner membrane protein (NCBI), at 1,127,062 to 1,128,597
mviN
b1070: flgN - export chaperone for FlgK and FlgL (NCBI), at 1,128,637 to 1,129,053
flgN
b1071: flgM - anti-sigma factor for FliA (sigma 28) (NCBI), at 1,129,058 to 1,129,351
flgM
b1072: flgA - flagellar basal body P-ring biosynthesis protein A (NCBI), at 1,129,427 to 1,130,086
flgA
b1073: flgB - flagellar basal-body rod protein B (NCBI), at 1,130,241 to 1,130,657
flgB
b1074: flgC - flagellar basal-body rod protein C (NCBI), at 1,130,661 to 1,131,065
flgC
b1075: flgD - flagellar basal body rod modification protein D (NCBI), at 1,131,077 to 1,131,772
flgD
Position (kb)
1129
1130
1131 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1128.553 kb on + strand at 1128.592 kb on + strand at 1128.600 kb on - strand at 1128.612 kb on + strand at 1128.932 kb on - strand, within flgN at 1129.057 kb on + strand at 1129.094 kb on - strand, within flgM at 1129.303 kb on + strand, within flgM at 1129.414 kb on - strand at 1129.433 kb on - strand at 1129.433 kb on - strand at 1129.579 kb on + strand, within flgA at 1129.663 kb on + strand, within flgA at 1129.716 kb on + strand, within flgA at 1129.771 kb on + strand, within flgA at 1129.771 kb on + strand, within flgA at 1129.810 kb on + strand, within flgA at 1129.811 kb on - strand, within flgA at 1129.813 kb on + strand, within flgA at 1129.815 kb on + strand, within flgA at 1129.815 kb on + strand, within flgA at 1129.958 kb on + strand, within flgA at 1130.045 kb on + strand at 1130.097 kb on + strand at 1130.110 kb on + strand at 1130.161 kb on + strand at 1130.236 kb on - strand at 1130.238 kb on - strand at 1130.238 kb on - strand at 1130.270 kb on - strand at 1130.352 kb on - strand, within flgB at 1130.352 kb on - strand, within flgB at 1130.411 kb on + strand, within flgB at 1130.411 kb on + strand, within flgB at 1130.456 kb on - strand, within flgB at 1130.456 kb on - strand, within flgB at 1130.513 kb on - strand, within flgB at 1130.570 kb on - strand, within flgB at 1130.693 kb on - strand at 1130.701 kb on + strand at 1131.014 kb on + strand, within flgC at 1131.014 kb on + strand, within flgC at 1131.054 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 1,128,553 + +2.7 1,128,592 + +0.5 1,128,600 - -0.8 1,128,612 + +1.6 1,128,932 - flgN b1070 0.71 +0.8 1,129,057 + -1.0 1,129,094 - flgM b1071 0.12 -0.4 1,129,303 + flgM b1071 0.83 +1.7 1,129,414 - -1.1 1,129,433 - -0.2 1,129,433 - +0.2 1,129,579 + flgA b1072 0.23 +1.2 1,129,663 + flgA b1072 0.36 +1.5 1,129,716 + flgA b1072 0.44 -0.0 1,129,771 + flgA b1072 0.52 -0.4 1,129,771 + flgA b1072 0.52 +2.0 1,129,810 + flgA b1072 0.58 +2.1 1,129,811 - flgA b1072 0.58 +0.5 1,129,813 + flgA b1072 0.58 -1.1 1,129,815 + flgA b1072 0.59 +2.0 1,129,815 + flgA b1072 0.59 -0.8 1,129,958 + flgA b1072 0.80 +1.6 1,130,045 + +0.0 1,130,097 + +1.0 1,130,110 + -0.4 1,130,161 + +0.0 1,130,236 - +1.0 1,130,238 - +2.5 1,130,238 - +1.6 1,130,270 - +0.1 1,130,352 - flgB b1073 0.27 -0.1 1,130,352 - flgB b1073 0.27 +0.7 1,130,411 + flgB b1073 0.41 +1.0 1,130,411 + flgB b1073 0.41 +0.2 1,130,456 - flgB b1073 0.52 +1.2 1,130,456 - flgB b1073 0.52 +1.0 1,130,513 - flgB b1073 0.65 -0.8 1,130,570 - flgB b1073 0.79 -1.1 1,130,693 - +1.2 1,130,701 + +0.0 1,131,014 + flgC b1074 0.87 -2.1 1,131,014 + flgC b1074 0.87 -0.4 1,131,054 - +1.7
Or see this region's nucleotide sequence