Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycdK and b1011 are separated by 11 nucleotides b1011 and b1012 overlap by 1 nucleotides b1012 and ycdC are separated by 230 nucleotides ycdC and putA are separated by 39 nucleotides
b1010: ycdK - hypothetical protein (NCBI), at 1,070,996 to 1,071,382
ycdK
b1011: b1011 - putative synthetase (VIMSS), at 1,071,394 to 1,072,086
b1011
b1012: b1012 - orf, hypothetical protein (VIMSS), at 1,072,086 to 1,073,234
b1012
b1013: ycdC - predicted DNA-binding transcriptional regulator (NCBI), at 1,073,465 to 1,074,103
ycdC
b1014: putA - fused DNA-binding transcriptional regulator/proline dehydrogenase/pyrroline-5-carboxylate dehydrogenase (NCBI), at 1,074,143 to 1,078,105
putA
Position (kb)
1072
1073
1074 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1071.230 kb on - strand, within ycdK at 1071.301 kb on - strand, within ycdK at 1071.301 kb on - strand, within ycdK at 1071.301 kb on - strand, within ycdK at 1071.319 kb on - strand, within ycdK at 1071.343 kb on - strand, within ycdK at 1071.343 kb on - strand, within ycdK at 1071.382 kb on + strand at 1071.696 kb on + strand, within b1011 at 1071.712 kb on + strand, within b1011 at 1071.737 kb on - strand, within b1011 at 1072.068 kb on + strand at 1072.068 kb on + strand at 1072.104 kb on - strand at 1072.104 kb on - strand at 1072.188 kb on + strand at 1072.404 kb on - strand, within b1012 at 1072.404 kb on - strand, within b1012 at 1072.477 kb on - strand, within b1012 at 1072.477 kb on - strand, within b1012 at 1072.730 kb on - strand, within b1012 at 1072.755 kb on - strand, within b1012 at 1073.238 kb on + strand at 1073.585 kb on - strand, within ycdC at 1073.585 kb on - strand, within ycdC at 1073.819 kb on - strand, within ycdC at 1073.908 kb on + strand, within ycdC at 1074.009 kb on - strand, within ycdC at 1074.073 kb on + strand at 1074.086 kb on - strand at 1074.088 kb on + strand at 1074.088 kb on + strand at 1074.126 kb on - strand at 1074.140 kb on + strand at 1074.195 kb on - strand at 1074.215 kb on - strand at 1074.218 kb on - strand at 1074.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 1,071,230 - ycdK b1010 0.60 +2.5 1,071,301 - ycdK b1010 0.79 +0.9 1,071,301 - ycdK b1010 0.79 +3.2 1,071,301 - ycdK b1010 0.79 +2.2 1,071,319 - ycdK b1010 0.83 +0.1 1,071,343 - ycdK b1010 0.90 -1.8 1,071,343 - ycdK b1010 0.90 -0.4 1,071,382 + +1.5 1,071,696 + b1011 b1011 0.44 +0.5 1,071,712 + b1011 b1011 0.46 +0.7 1,071,737 - b1011 b1011 0.49 +0.9 1,072,068 + +2.4 1,072,068 + +2.8 1,072,104 - +1.6 1,072,104 - +1.5 1,072,188 + +2.2 1,072,404 - b1012 b1012 0.28 -1.1 1,072,404 - b1012 b1012 0.28 -1.6 1,072,477 - b1012 b1012 0.34 -2.5 1,072,477 - b1012 b1012 0.34 +0.2 1,072,730 - b1012 b1012 0.56 +0.5 1,072,755 - b1012 b1012 0.58 -1.8 1,073,238 + -1.1 1,073,585 - ycdC b1013 0.19 +0.5 1,073,585 - ycdC b1013 0.19 -1.3 1,073,819 - ycdC b1013 0.55 +0.4 1,073,908 + ycdC b1013 0.69 +2.3 1,074,009 - ycdC b1013 0.85 +1.0 1,074,073 + +2.0 1,074,086 - +0.8 1,074,088 + -1.3 1,074,088 + -1.3 1,074,126 - +1.1 1,074,140 + +1.6 1,074,195 - +1.5 1,074,215 - -0.9 1,074,218 - -0.9 1,074,218 - -0.7
Or see this region's nucleotide sequence