Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pqiA and pqiB are separated by 4 nucleotides pqiB and ymbA overlap by 4 nucleotides ymbA and rmf are separated by 255 nucleotides
b0950: pqiA - paraquat-inducible membrane protein A (NCBI), at 1,011,224 to 1,012,477
pqiA
b0951: pqiB - paraquat-inducible protein B (NCBI), at 1,012,482 to 1,014,122
pqiB
b0952: ymbA - conserved protein (RefSeq), at 1,014,119 to 1,014,682
ymbA
b0953: rmf - ribosome modulation factor (NCBI), at 1,014,938 to 1,015,105
rmf
Position (kb)
1012
1013
1014
1015 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1011.607 kb on - strand, within pqiA at 1011.620 kb on - strand, within pqiA at 1011.748 kb on + strand, within pqiA at 1011.794 kb on + strand, within pqiA at 1011.876 kb on + strand, within pqiA at 1011.956 kb on - strand, within pqiA at 1011.982 kb on + strand, within pqiA at 1012.008 kb on - strand, within pqiA at 1012.008 kb on - strand, within pqiA at 1012.011 kb on + strand, within pqiA at 1012.067 kb on + strand, within pqiA at 1012.067 kb on + strand, within pqiA at 1012.131 kb on - strand, within pqiA at 1012.166 kb on - strand, within pqiA at 1012.166 kb on - strand, within pqiA at 1012.181 kb on - strand, within pqiA at 1012.260 kb on - strand, within pqiA at 1012.323 kb on - strand, within pqiA at 1012.323 kb on - strand, within pqiA at 1012.450 kb on + strand at 1012.450 kb on + strand at 1012.460 kb on + strand at 1012.570 kb on - strand at 1012.570 kb on - strand at 1012.625 kb on - strand at 1012.625 kb on - strand at 1012.672 kb on + strand, within pqiB at 1012.805 kb on + strand, within pqiB at 1012.847 kb on + strand, within pqiB at 1012.865 kb on - strand, within pqiB at 1012.865 kb on - strand, within pqiB at 1013.007 kb on + strand, within pqiB at 1013.020 kb on - strand, within pqiB at 1013.020 kb on - strand, within pqiB at 1013.031 kb on - strand, within pqiB at 1013.108 kb on - strand, within pqiB at 1013.119 kb on + strand, within pqiB at 1013.144 kb on + strand, within pqiB at 1013.168 kb on - strand, within pqiB at 1013.178 kb on - strand, within pqiB at 1013.178 kb on - strand, within pqiB at 1013.211 kb on - strand, within pqiB at 1013.329 kb on - strand, within pqiB at 1013.374 kb on + strand, within pqiB at 1013.374 kb on + strand, within pqiB at 1013.389 kb on - strand, within pqiB at 1013.399 kb on + strand, within pqiB at 1013.632 kb on - strand, within pqiB at 1013.632 kb on - strand, within pqiB at 1013.633 kb on - strand, within pqiB at 1013.634 kb on - strand, within pqiB at 1013.635 kb on - strand, within pqiB at 1013.730 kb on + strand, within pqiB at 1013.738 kb on - strand, within pqiB at 1013.811 kb on - strand, within pqiB at 1013.811 kb on - strand, within pqiB at 1013.858 kb on - strand, within pqiB at 1013.858 kb on - strand, within pqiB at 1013.949 kb on - strand, within pqiB at 1013.955 kb on + strand, within pqiB at 1013.955 kb on + strand, within pqiB at 1014.064 kb on - strand at 1014.064 kb on - strand at 1014.159 kb on - strand at 1014.206 kb on + strand, within ymbA at 1014.216 kb on + strand, within ymbA at 1014.216 kb on + strand, within ymbA at 1014.263 kb on + strand, within ymbA at 1014.268 kb on + strand, within ymbA at 1014.303 kb on - strand, within ymbA at 1014.428 kb on - strand, within ymbA at 1014.428 kb on - strand, within ymbA at 1014.587 kb on + strand, within ymbA at 1014.588 kb on + strand, within ymbA at 1014.588 kb on + strand, within ymbA at 1014.619 kb on - strand, within ymbA at 1014.959 kb on + strand, within rmf at 1014.974 kb on + strand, within rmf at 1014.974 kb on + strand, within rmf at 1015.089 kb on + strand at 1015.097 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 1,011,607 - pqiA b0950 0.31 -1.8 1,011,620 - pqiA b0950 0.32 -1.5 1,011,748 + pqiA b0950 0.42 -2.1 1,011,794 + pqiA b0950 0.45 +1.3 1,011,876 + pqiA b0950 0.52 +0.3 1,011,956 - pqiA b0950 0.58 +0.3 1,011,982 + pqiA b0950 0.60 -2.8 1,012,008 - pqiA b0950 0.63 -0.9 1,012,008 - pqiA b0950 0.63 -0.9 1,012,011 + pqiA b0950 0.63 -1.9 1,012,067 + pqiA b0950 0.67 -1.4 1,012,067 + pqiA b0950 0.67 -1.7 1,012,131 - pqiA b0950 0.72 -2.9 1,012,166 - pqiA b0950 0.75 -1.3 1,012,166 - pqiA b0950 0.75 -1.7 1,012,181 - pqiA b0950 0.76 -0.7 1,012,260 - pqiA b0950 0.83 -2.1 1,012,323 - pqiA b0950 0.88 -1.3 1,012,323 - pqiA b0950 0.88 -1.1 1,012,450 + +1.7 1,012,450 + +0.3 1,012,460 + +1.3 1,012,570 - -0.7 1,012,570 - -0.3 1,012,625 - -0.5 1,012,625 - +1.0 1,012,672 + pqiB b0951 0.12 -0.3 1,012,805 + pqiB b0951 0.20 -1.3 1,012,847 + pqiB b0951 0.22 -2.4 1,012,865 - pqiB b0951 0.23 +0.3 1,012,865 - pqiB b0951 0.23 -0.7 1,013,007 + pqiB b0951 0.32 -0.6 1,013,020 - pqiB b0951 0.33 -0.7 1,013,020 - pqiB b0951 0.33 -2.7 1,013,031 - pqiB b0951 0.33 -1.6 1,013,108 - pqiB b0951 0.38 +0.3 1,013,119 + pqiB b0951 0.39 -1.9 1,013,144 + pqiB b0951 0.40 +0.3 1,013,168 - pqiB b0951 0.42 +0.3 1,013,178 - pqiB b0951 0.42 -1.3 1,013,178 - pqiB b0951 0.42 -0.9 1,013,211 - pqiB b0951 0.44 -0.3 1,013,329 - pqiB b0951 0.52 -0.3 1,013,374 + pqiB b0951 0.54 -0.1 1,013,374 + pqiB b0951 0.54 -0.2 1,013,389 - pqiB b0951 0.55 -1.3 1,013,399 + pqiB b0951 0.56 -0.7 1,013,632 - pqiB b0951 0.70 -1.5 1,013,632 - pqiB b0951 0.70 -2.5 1,013,633 - pqiB b0951 0.70 +0.5 1,013,634 - pqiB b0951 0.70 -1.7 1,013,635 - pqiB b0951 0.70 -1.2 1,013,730 + pqiB b0951 0.76 -0.9 1,013,738 - pqiB b0951 0.77 -1.9 1,013,811 - pqiB b0951 0.81 -0.5 1,013,811 - pqiB b0951 0.81 -2.3 1,013,858 - pqiB b0951 0.84 -1.3 1,013,858 - pqiB b0951 0.84 +0.1 1,013,949 - pqiB b0951 0.89 -1.3 1,013,955 + pqiB b0951 0.90 -1.7 1,013,955 + pqiB b0951 0.90 -1.3 1,014,064 - -0.1 1,014,064 - -1.4 1,014,159 - -0.3 1,014,206 + ymbA b0952 0.15 -1.5 1,014,216 + ymbA b0952 0.17 -1.5 1,014,216 + ymbA b0952 0.17 +2.3 1,014,263 + ymbA b0952 0.26 -1.4 1,014,268 + ymbA b0952 0.26 -1.3 1,014,303 - ymbA b0952 0.33 -1.7 1,014,428 - ymbA b0952 0.55 -0.3 1,014,428 - ymbA b0952 0.55 -1.1 1,014,587 + ymbA b0952 0.83 -2.7 1,014,588 + ymbA b0952 0.83 -1.1 1,014,588 + ymbA b0952 0.83 -2.3 1,014,619 - ymbA b0952 0.89 -2.5 1,014,959 + rmf b0953 0.12 +0.5 1,014,974 + rmf b0953 0.21 +1.8 1,014,974 + rmf b0953 0.21 -0.3 1,015,089 + -1.1 1,015,097 - +2.7
Or see this region's nucleotide sequence