Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mipB and moeB are separated by 75 nucleotides moeB and moeA overlap by 1 nucleotides moeA and iaaA are separated by 203 nucleotides
b0825: mipB - putative transaldolase (VIMSS), at 862,865 to 863,527
mipB
b0826: moeB - molybdopterin biosynthesis protein MoeB (NCBI), at 863,603 to 864,352
moeB
b0827: moeA - molybdopterin biosynthesis protein (NCBI), at 864,352 to 865,587
moeA
b0828: iaaA - L-asparaginase (NCBI), at 865,791 to 866,756
iaaA
Position (kb)
864
865
866 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 863.433 kb on + strand, within mipB at 863.554 kb on + strand at 863.625 kb on - strand at 863.673 kb on - strand at 863.881 kb on + strand, within moeB at 863.881 kb on + strand, within moeB at 863.980 kb on - strand, within moeB at 863.987 kb on + strand, within moeB at 864.031 kb on - strand, within moeB at 864.031 kb on - strand, within moeB at 864.040 kb on - strand, within moeB at 864.067 kb on + strand, within moeB at 864.071 kb on - strand, within moeB at 864.129 kb on + strand, within moeB at 864.167 kb on + strand, within moeB at 864.209 kb on - strand, within moeB at 864.209 kb on - strand, within moeB at 864.214 kb on - strand, within moeB at 864.271 kb on - strand, within moeB at 864.647 kb on + strand, within moeA at 864.770 kb on - strand, within moeA at 864.770 kb on - strand, within moeA at 864.814 kb on - strand, within moeA at 864.855 kb on + strand, within moeA at 864.945 kb on + strand, within moeA at 865.047 kb on + strand, within moeA at 865.064 kb on - strand, within moeA at 865.220 kb on + strand, within moeA at 865.243 kb on + strand, within moeA at 865.243 kb on + strand, within moeA at 865.278 kb on - strand, within moeA at 865.278 kb on - strand at 865.301 kb on - strand, within moeA at 865.527 kb on + strand at 865.527 kb on + strand at 865.535 kb on - strand at 865.561 kb on - strand at 865.646 kb on + strand at 865.646 kb on + strand at 865.648 kb on + strand at 865.771 kb on + strand at 865.771 kb on + strand at 865.875 kb on + strand at 865.883 kb on + strand at 866.064 kb on - strand, within iaaA at 866.149 kb on + strand, within iaaA at 866.246 kb on + strand at 866.424 kb on + strand, within iaaA at 866.432 kb on - strand, within iaaA at 866.537 kb on + strand, within iaaA
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 863,433 + mipB b0825 0.86 -1.0 863,554 + +0.2 863,625 - +0.9 863,673 - +2.8 863,881 + moeB b0826 0.37 -0.5 863,881 + moeB b0826 0.37 +2.4 863,980 - moeB b0826 0.50 +2.4 863,987 + moeB b0826 0.51 +4.2 864,031 - moeB b0826 0.57 +3.5 864,031 - moeB b0826 0.57 +2.1 864,040 - moeB b0826 0.58 +2.1 864,067 + moeB b0826 0.62 +2.0 864,071 - moeB b0826 0.62 +2.6 864,129 + moeB b0826 0.70 +3.6 864,167 + moeB b0826 0.75 +2.4 864,209 - moeB b0826 0.81 +2.4 864,209 - moeB b0826 0.81 +2.6 864,214 - moeB b0826 0.81 +2.9 864,271 - moeB b0826 0.89 +2.5 864,647 + moeA b0827 0.24 +3.6 864,770 - moeA b0827 0.34 +2.5 864,770 - moeA b0827 0.34 -0.0 864,814 - moeA b0827 0.37 +0.8 864,855 + moeA b0827 0.41 +2.6 864,945 + moeA b0827 0.48 +1.5 865,047 + moeA b0827 0.56 +1.5 865,064 - moeA b0827 0.58 +4.1 865,220 + moeA b0827 0.70 +1.5 865,243 + moeA b0827 0.72 +1.9 865,243 + moeA b0827 0.72 +2.3 865,278 - moeA b0827 0.75 -0.3 865,278 - +1.3 865,301 - moeA b0827 0.77 +1.5 865,527 + +3.0 865,527 + +3.3 865,535 - +2.2 865,561 - +1.8 865,646 + +0.1 865,646 + -0.4 865,648 + +0.8 865,771 + +1.3 865,771 + +0.7 865,875 + +1.0 865,883 + +1.7 866,064 - iaaA b0828 0.28 +3.3 866,149 + iaaA b0828 0.37 +0.3 866,246 + -0.7 866,424 + iaaA b0828 0.66 +0.1 866,432 - iaaA b0828 0.66 -1.3 866,537 + iaaA b0828 0.77 -0.7
Or see this region's nucleotide sequence