Strain Fitness in Escherichia coli BW25113 around b0827

Experiment: Benzalkonium Chloride 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmipB and moeB are separated by 75 nucleotidesmoeB and moeA overlap by 1 nucleotidesmoeA and iaaA are separated by 203 nucleotides b0825: mipB - putative transaldolase (VIMSS), at 862,865 to 863,527 mipB b0826: moeB - molybdopterin biosynthesis protein MoeB (NCBI), at 863,603 to 864,352 moeB b0827: moeA - molybdopterin biosynthesis protein (NCBI), at 864,352 to 865,587 moeA b0828: iaaA - L-asparaginase (NCBI), at 865,791 to 866,756 iaaA Position (kb) 864 865 866Strain fitness (log2 ratio) -1 0 1 2 3 4at 863.433 kb on + strand, within mipBat 863.554 kb on + strandat 863.625 kb on - strandat 863.673 kb on - strandat 863.881 kb on + strand, within moeBat 863.881 kb on + strand, within moeBat 863.980 kb on - strand, within moeBat 863.987 kb on + strand, within moeBat 864.031 kb on - strand, within moeBat 864.031 kb on - strand, within moeBat 864.040 kb on - strand, within moeBat 864.067 kb on + strand, within moeBat 864.071 kb on - strand, within moeBat 864.129 kb on + strand, within moeBat 864.167 kb on + strand, within moeBat 864.209 kb on - strand, within moeBat 864.209 kb on - strand, within moeBat 864.214 kb on - strand, within moeBat 864.271 kb on - strand, within moeBat 864.647 kb on + strand, within moeAat 864.770 kb on - strand, within moeAat 864.770 kb on - strand, within moeAat 864.814 kb on - strand, within moeAat 864.855 kb on + strand, within moeAat 864.945 kb on + strand, within moeAat 865.047 kb on + strand, within moeAat 865.064 kb on - strand, within moeAat 865.220 kb on + strand, within moeAat 865.243 kb on + strand, within moeAat 865.243 kb on + strand, within moeAat 865.278 kb on - strand, within moeAat 865.278 kb on - strandat 865.301 kb on - strand, within moeAat 865.527 kb on + strandat 865.527 kb on + strandat 865.535 kb on - strandat 865.561 kb on - strandat 865.646 kb on + strandat 865.646 kb on + strandat 865.648 kb on + strandat 865.771 kb on + strandat 865.771 kb on + strandat 865.875 kb on + strandat 865.883 kb on + strandat 866.064 kb on - strand, within iaaAat 866.149 kb on + strand, within iaaAat 866.246 kb on + strandat 866.424 kb on + strand, within iaaAat 866.432 kb on - strand, within iaaAat 866.537 kb on + strand, within iaaA

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml
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863,433 + mipB b0825 0.86 -1.0
863,554 + +0.2
863,625 - +0.9
863,673 - +2.8
863,881 + moeB b0826 0.37 -0.5
863,881 + moeB b0826 0.37 +2.4
863,980 - moeB b0826 0.50 +2.4
863,987 + moeB b0826 0.51 +4.2
864,031 - moeB b0826 0.57 +3.5
864,031 - moeB b0826 0.57 +2.1
864,040 - moeB b0826 0.58 +2.1
864,067 + moeB b0826 0.62 +2.0
864,071 - moeB b0826 0.62 +2.6
864,129 + moeB b0826 0.70 +3.6
864,167 + moeB b0826 0.75 +2.4
864,209 - moeB b0826 0.81 +2.4
864,209 - moeB b0826 0.81 +2.6
864,214 - moeB b0826 0.81 +2.9
864,271 - moeB b0826 0.89 +2.5
864,647 + moeA b0827 0.24 +3.6
864,770 - moeA b0827 0.34 +2.5
864,770 - moeA b0827 0.34 -0.0
864,814 - moeA b0827 0.37 +0.8
864,855 + moeA b0827 0.41 +2.6
864,945 + moeA b0827 0.48 +1.5
865,047 + moeA b0827 0.56 +1.5
865,064 - moeA b0827 0.58 +4.1
865,220 + moeA b0827 0.70 +1.5
865,243 + moeA b0827 0.72 +1.9
865,243 + moeA b0827 0.72 +2.3
865,278 - moeA b0827 0.75 -0.3
865,278 - +1.3
865,301 - moeA b0827 0.77 +1.5
865,527 + +3.0
865,527 + +3.3
865,535 - +2.2
865,561 - +1.8
865,646 + +0.1
865,646 + -0.4
865,648 + +0.8
865,771 + +1.3
865,771 + +0.7
865,875 + +1.0
865,883 + +1.7
866,064 - iaaA b0828 0.28 +3.3
866,149 + iaaA b0828 0.37 +0.3
866,246 + -0.7
866,424 + iaaA b0828 0.66 +0.1
866,432 - iaaA b0828 0.66 -1.3
866,537 + iaaA b0828 0.77 -0.7

Or see this region's nucleotide sequence