Strain Fitness in Escherichia coli BW25113 around b0638

Experiment: Benzalkonium Chloride 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybeA and ybeB are separated by 3 nucleotidesybeB and cobC are separated by 259 nucleotidescobC and nadD are separated by 23 nucleotidesnadD and holA are separated by 1 nucleotides b0636: ybeA - hypothetical protein (NCBI), at 667,471 to 667,938 ybeA b0637: ybeB - orf, hypothetical protein (VIMSS), at 667,942 to 668,259 ybeB b0638: cobC - predicted alpha-ribazole-5'-P phosphatase (NCBI), at 668,519 to 669,130 cobC b0639: nadD - nicotinic acid mononucleotide adenyltransferase (NCBI), at 669,154 to 669,795 nadD b0640: holA - DNA polymerase III subunit delta (NCBI), at 669,797 to 670,828 holA Position (kb) 668 669 670Strain fitness (log2 ratio) -2 -1 0 1 2 3at 667.524 kb on - strand, within ybeAat 667.524 kb on - strand, within ybeAat 667.588 kb on - strand, within ybeAat 667.590 kb on - strand, within ybeAat 667.665 kb on - strand, within ybeAat 667.665 kb on - strand, within ybeAat 667.703 kb on - strand, within ybeAat 667.703 kb on - strand, within ybeAat 667.762 kb on - strand, within ybeAat 667.762 kb on - strand, within ybeAat 667.852 kb on - strand, within ybeAat 667.852 kb on - strand, within ybeAat 668.424 kb on + strandat 668.466 kb on - strandat 668.466 kb on - strandat 668.601 kb on - strand, within cobCat 668.672 kb on + strand, within cobCat 668.680 kb on - strand, within cobCat 668.733 kb on + strand, within cobCat 668.733 kb on + strand, within cobCat 668.796 kb on - strand, within cobCat 668.796 kb on - strand, within cobCat 668.930 kb on + strand, within cobCat 668.948 kb on + strand, within cobCat 668.948 kb on + strand, within cobCat 668.992 kb on + strand, within cobCat 669.031 kb on + strand, within cobCat 669.048 kb on + strand, within cobCat 669.057 kb on - strand, within cobCat 669.057 kb on - strand, within cobCat 669.095 kb on - strandat 669.153 kb on - strandat 669.158 kb on - strandat 669.158 kb on - strandat 669.179 kb on + strandat 669.187 kb on - strandat 669.852 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml
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667,524 - ybeA b0636 0.11 +0.3
667,524 - ybeA b0636 0.11 +0.2
667,588 - ybeA b0636 0.25 +0.6
667,590 - ybeA b0636 0.25 +0.2
667,665 - ybeA b0636 0.41 -1.5
667,665 - ybeA b0636 0.41 +2.7
667,703 - ybeA b0636 0.50 +1.3
667,703 - ybeA b0636 0.50 -2.1
667,762 - ybeA b0636 0.62 +2.8
667,762 - ybeA b0636 0.62 -0.7
667,852 - ybeA b0636 0.81 +2.3
667,852 - ybeA b0636 0.81 +2.1
668,424 + -1.2
668,466 - -1.2
668,466 - +0.9
668,601 - cobC b0638 0.13 -1.2
668,672 + cobC b0638 0.25 -1.4
668,680 - cobC b0638 0.26 +0.9
668,733 + cobC b0638 0.35 -2.7
668,733 + cobC b0638 0.35 -0.4
668,796 - cobC b0638 0.45 +1.4
668,796 - cobC b0638 0.45 -1.2
668,930 + cobC b0638 0.67 +1.5
668,948 + cobC b0638 0.70 +1.4
668,948 + cobC b0638 0.70 +0.4
668,992 + cobC b0638 0.77 +1.4
669,031 + cobC b0638 0.84 +0.6
669,048 + cobC b0638 0.86 +0.2
669,057 - cobC b0638 0.88 +0.2
669,057 - cobC b0638 0.88 -0.4
669,095 - -1.2
669,153 - -0.5
669,158 - -1.0
669,158 - -2.7
669,179 + -1.8
669,187 - -1.0
669,852 + -1.3

Or see this region's nucleotide sequence