Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdH and ybdL are separated by 108 nucleotides ybdL and ybdM are separated by 0 nucleotides ybdM and ybdN overlap by 28 nucleotides
b0599: ybdH - predicted oxidoreductase (NCBI), at 631,612 to 632,700
ybdH
b0600: ybdL - putative aminotransferase (NCBI), at 632,809 to 633,969
ybdL
b0601: ybdM - hypothetical protein (NCBI), at 633,970 to 634,599
ybdM
b0602: ybdN - hypothetical protein (NCBI), at 634,572 to 635,792
ybdN
Position (kb)
632
633
634 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 631.825 kb on + strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.926 kb on + strand, within ybdH at 631.926 kb on - strand, within ybdH at 632.035 kb on + strand, within ybdH at 632.048 kb on + strand, within ybdH at 632.147 kb on + strand, within ybdH at 632.160 kb on + strand, within ybdH at 632.399 kb on + strand, within ybdH at 632.466 kb on - strand, within ybdH at 632.581 kb on + strand, within ybdH at 632.877 kb on - strand at 632.877 kb on - strand at 632.948 kb on + strand, within ybdL at 633.223 kb on + strand, within ybdL at 633.228 kb on + strand, within ybdL at 633.228 kb on + strand, within ybdL at 633.228 kb on - strand, within ybdL at 633.320 kb on - strand, within ybdL at 633.322 kb on - strand, within ybdL at 633.327 kb on - strand, within ybdL at 633.351 kb on - strand, within ybdL at 633.418 kb on + strand, within ybdL at 633.418 kb on - strand, within ybdL at 633.418 kb on - strand, within ybdL at 633.442 kb on - strand, within ybdL at 633.442 kb on - strand, within ybdL at 633.541 kb on - strand, within ybdL at 633.551 kb on - strand, within ybdL at 633.570 kb on - strand, within ybdL at 633.572 kb on + strand, within ybdL at 633.587 kb on + strand, within ybdL at 633.597 kb on - strand, within ybdL at 633.717 kb on - strand, within ybdL at 633.825 kb on + strand, within ybdL at 633.853 kb on - strand, within ybdL at 633.970 kb on + strand at 634.194 kb on + strand, within ybdM at 634.206 kb on + strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.309 kb on + strand, within ybdM at 634.335 kb on - strand, within ybdM at 634.335 kb on - strand, within ybdM at 634.337 kb on - strand, within ybdM at 634.464 kb on - strand, within ybdM at 634.477 kb on - strand, within ybdM at 634.486 kb on - strand, within ybdM at 634.486 kb on - strand, within ybdM at 634.593 kb on + strand at 634.672 kb on + strand at 634.687 kb on + strand at 634.720 kb on - strand, within ybdN at 634.963 kb on + strand, within ybdN
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 631,825 + ybdH b0599 0.20 -0.5 631,828 - ybdH b0599 0.20 +0.9 631,828 - ybdH b0599 0.20 +0.4 631,833 - ybdH b0599 0.20 -2.1 631,833 - ybdH b0599 0.20 +0.4 631,926 + ybdH b0599 0.29 -0.5 631,926 - ybdH b0599 0.29 -0.9 632,035 + ybdH b0599 0.39 -0.2 632,048 + ybdH b0599 0.40 +0.3 632,147 + ybdH b0599 0.49 -0.4 632,160 + ybdH b0599 0.50 +0.6 632,399 + ybdH b0599 0.72 -2.6 632,466 - ybdH b0599 0.78 -0.9 632,581 + ybdH b0599 0.89 +1.1 632,877 - -0.2 632,877 - -0.6 632,948 + ybdL b0600 0.12 -0.7 633,223 + ybdL b0600 0.36 +1.3 633,228 + ybdL b0600 0.36 -2.2 633,228 + ybdL b0600 0.36 +1.4 633,228 - ybdL b0600 0.36 -2.5 633,320 - ybdL b0600 0.44 -0.9 633,322 - ybdL b0600 0.44 -0.4 633,327 - ybdL b0600 0.45 -1.8 633,351 - ybdL b0600 0.47 -0.6 633,418 + ybdL b0600 0.52 +0.4 633,418 - ybdL b0600 0.52 +0.5 633,418 - ybdL b0600 0.52 -1.1 633,442 - ybdL b0600 0.55 +0.1 633,442 - ybdL b0600 0.55 -1.9 633,541 - ybdL b0600 0.63 -1.4 633,551 - ybdL b0600 0.64 +1.1 633,570 - ybdL b0600 0.66 -0.9 633,572 + ybdL b0600 0.66 +1.3 633,587 + ybdL b0600 0.67 -0.1 633,597 - ybdL b0600 0.68 -0.8 633,717 - ybdL b0600 0.78 -0.6 633,825 + ybdL b0600 0.88 -0.2 633,853 - ybdL b0600 0.90 +0.1 633,970 + -0.8 634,194 + ybdM b0601 0.36 +1.1 634,206 + ybdM b0601 0.37 +0.1 634,214 - ybdM b0601 0.39 +1.4 634,214 - ybdM b0601 0.39 +0.6 634,214 - ybdM b0601 0.39 -1.1 634,214 - ybdM b0601 0.39 +2.1 634,214 - ybdM b0601 0.39 -0.5 634,309 + ybdM b0601 0.54 -1.8 634,335 - ybdM b0601 0.58 -2.2 634,335 - ybdM b0601 0.58 +0.4 634,337 - ybdM b0601 0.58 +0.3 634,464 - ybdM b0601 0.78 +0.2 634,477 - ybdM b0601 0.80 +0.7 634,486 - ybdM b0601 0.82 +0.6 634,486 - ybdM b0601 0.82 +0.8 634,593 + +0.4 634,672 + +1.9 634,687 + -1.4 634,720 - ybdN b0602 0.12 +2.2 634,963 + ybdN b0602 0.32 +1.6
Or see this region's nucleotide sequence