Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt entD and fepA are separated by 174 nucleotides fepA and fes are separated by 320 nucleotides
b0583: entD - phosphopantetheinyltransferase component of enterobactin synthase multienzyme complex (NCBI), at 608,682 to 609,302
entD
b0584: fepA - iron-enterobactin outer membrane transporter (NCBI), at 609,477 to 611,717
fepA
b0585: fes - enterobactin/ferric enterobactin esterase (NCBI), at 612,038 to 613,162
fes
Position (kb) 609
610
611
612 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 608.621 kb on + strand at 608.621 kb on + strand at 608.621 kb on + strand at 608.629 kb on - strand at 608.669 kb on - strand at 608.750 kb on - strand, within entD at 608.761 kb on + strand, within entD at 608.766 kb on + strand, within entD at 608.834 kb on + strand, within entD at 609.183 kb on + strand, within entD at 609.246 kb on - strand at 609.246 kb on - strand at 609.253 kb on + strand at 609.253 kb on + strand at 609.266 kb on + strand at 609.266 kb on + strand at 609.277 kb on - strand at 609.277 kb on - strand at 609.284 kb on - strand at 609.303 kb on - strand at 609.304 kb on - strand at 609.338 kb on - strand at 609.338 kb on - strand at 609.498 kb on + strand at 609.536 kb on - strand at 609.549 kb on - strand at 609.580 kb on + strand at 609.597 kb on - strand at 609.597 kb on - strand at 609.724 kb on - strand, within fepA at 609.966 kb on - strand, within fepA at 610.025 kb on + strand, within fepA at 610.193 kb on - strand, within fepA at 610.193 kb on - strand, within fepA at 610.202 kb on - strand, within fepA at 610.337 kb on + strand, within fepA at 610.443 kb on - strand, within fepA at 610.445 kb on + strand, within fepA at 610.456 kb on + strand, within fepA at 610.510 kb on - strand, within fepA at 610.719 kb on + strand, within fepA at 610.719 kb on + strand, within fepA at 610.719 kb on + strand, within fepA at 610.730 kb on - strand, within fepA at 610.737 kb on + strand, within fepA at 610.793 kb on + strand, within fepA at 610.840 kb on + strand, within fepA at 610.840 kb on - strand, within fepA at 610.878 kb on - strand, within fepA at 610.958 kb on - strand, within fepA at 610.964 kb on + strand, within fepA at 611.112 kb on + strand, within fepA at 611.148 kb on - strand, within fepA at 611.188 kb on - strand, within fepA at 611.322 kb on + strand, within fepA at 611.500 kb on + strand at 611.500 kb on + strand at 611.500 kb on + strand at 612.036 kb on - strand at 612.036 kb on - strand at 612.112 kb on + strand at 612.112 kb on + strand at 612.117 kb on - strand at 612.132 kb on + strand at 612.132 kb on + strand at 612.203 kb on - strand, within fes at 612.203 kb on - strand, within fes at 612.234 kb on + strand, within fes at 612.272 kb on - strand, within fes at 612.424 kb on + strand, within fes at 612.424 kb on + strand, within fes at 612.629 kb on - strand, within fes at 612.629 kb on - strand, within fes at 612.676 kb on - strand, within fes
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 608,621 + -1.6 608,621 + -1.2 608,621 + -0.9 608,629 - +1.0 608,669 - +1.8 608,750 - entD b0583 0.11 -0.1 608,761 + entD b0583 0.13 -1.2 608,766 + entD b0583 0.14 -0.2 608,834 + entD b0583 0.24 +0.8 609,183 + entD b0583 0.81 -1.2 609,246 - -1.2 609,246 - -0.2 609,253 + -0.8 609,253 + +0.2 609,266 + +0.4 609,266 + -1.2 609,277 - -0.9 609,277 - +0.4 609,284 - +0.9 609,303 - -1.6 609,304 - -2.8 609,338 - +0.8 609,338 - +1.6 609,498 + +0.8 609,536 - -1.9 609,549 - +1.0 609,580 + -1.8 609,597 - +1.2 609,597 - -0.2 609,724 - fepA b0584 0.11 -1.5 609,966 - fepA b0584 0.22 -1.6 610,025 + fepA b0584 0.24 +1.1 610,193 - fepA b0584 0.32 -1.4 610,193 - fepA b0584 0.32 -3.3 610,202 - fepA b0584 0.32 -1.7 610,337 + fepA b0584 0.38 +0.5 610,443 - fepA b0584 0.43 +0.1 610,445 + fepA b0584 0.43 -0.9 610,456 + fepA b0584 0.44 +0.5 610,510 - fepA b0584 0.46 -0.2 610,719 + fepA b0584 0.55 -1.3 610,719 + fepA b0584 0.55 -0.6 610,719 + fepA b0584 0.55 -1.9 610,730 - fepA b0584 0.56 -2.2 610,737 + fepA b0584 0.56 +0.8 610,793 + fepA b0584 0.59 +1.4 610,840 + fepA b0584 0.61 -0.9 610,840 - fepA b0584 0.61 +0.4 610,878 - fepA b0584 0.63 -1.6 610,958 - fepA b0584 0.66 +1.4 610,964 + fepA b0584 0.66 +0.1 611,112 + fepA b0584 0.73 +3.4 611,148 - fepA b0584 0.75 +0.3 611,188 - fepA b0584 0.76 +0.2 611,322 + fepA b0584 0.82 -1.1 611,500 + -0.5 611,500 + +3.2 611,500 + +3.0 612,036 - -0.7 612,036 - -1.3 612,112 + +0.4 612,112 + -1.6 612,117 - +0.4 612,132 + -1.8 612,132 + +1.1 612,203 - fes b0585 0.15 -0.6 612,203 - fes b0585 0.15 -2.1 612,234 + fes b0585 0.17 -2.9 612,272 - fes b0585 0.21 +0.4 612,424 + fes b0585 0.34 -0.2 612,424 + fes b0585 0.34 -1.8 612,629 - fes b0585 0.53 -0.6 612,629 - fes b0585 0.53 -0.6 612,676 - fes b0585 0.57 -1.4
Or see this region's nucleotide sequence