Strain Fitness in Escherichia coli BW25113 around b0474

Experiment: Benzalkonium Chloride 0.004 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthtpG and adk are separated by 180 nucleotidesadk and hemH are separated by 235 nucleotides b0473: htpG - heat shock protein 90 (NCBI), at 494,344 to 496,218 htpG b0474: adk - adenylate kinase (NCBI), at 496,399 to 497,043 adk b0475: hemH - ferrochelatase (NCBI), at 497,279 to 498,241 hemH Position (kb) 496 497 498Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 495.497 kb on - strand, within htpGat 495.526 kb on - strand, within htpGat 495.622 kb on - strand, within htpGat 495.643 kb on + strand, within htpGat 495.643 kb on + strand, within htpGat 495.651 kb on - strand, within htpGat 495.653 kb on + strand, within htpGat 495.661 kb on + strand, within htpGat 495.661 kb on + strand, within htpGat 495.763 kb on - strand, within htpGat 495.783 kb on - strand, within htpGat 495.968 kb on + strand, within htpGat 496.118 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml
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495,497 - htpG b0473 0.61 -2.1
495,526 - htpG b0473 0.63 -0.3
495,622 - htpG b0473 0.68 -1.2
495,643 + htpG b0473 0.69 -0.8
495,643 + htpG b0473 0.69 -0.7
495,651 - htpG b0473 0.70 -2.1
495,653 + htpG b0473 0.70 -2.7
495,661 + htpG b0473 0.70 +0.2
495,661 + htpG b0473 0.70 -2.8
495,763 - htpG b0473 0.76 -1.0
495,783 - htpG b0473 0.77 +0.5
495,968 + htpG b0473 0.87 +1.8
496,118 + +1.1

Or see this region's nucleotide sequence