Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yahF and yahG overlap by 1 nucleotides yahG and yahI are separated by 421 nucleotides
b0320: yahF - predicted acyl-CoA synthetase with NAD(P)-binding domain and succinyl-CoA synthetase domain (NCBI), at 336,002 to 337,549
yahF
b0321: yahG - hypothetical protein (NCBI), at 337,549 to 338,967
yahG
b0323: yahI - predicted carbamate kinase-like protein (NCBI), at 339,389 to 340,339
yahI
Position (kb) 337
338
339 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 336.554 kb on - strand, within yahF at 337.024 kb on - strand, within yahF at 337.163 kb on - strand, within yahF at 337.169 kb on + strand, within yahF at 337.169 kb on + strand, within yahF at 337.236 kb on - strand, within yahF at 337.603 kb on + strand at 337.603 kb on + strand at 337.763 kb on + strand, within yahG at 337.763 kb on + strand, within yahG at 337.821 kb on + strand, within yahG at 337.829 kb on - strand, within yahG at 337.974 kb on - strand, within yahG at 337.974 kb on - strand, within yahG at 338.157 kb on + strand, within yahG at 338.157 kb on + strand, within yahG at 338.178 kb on + strand, within yahG at 338.183 kb on + strand, within yahG at 338.183 kb on + strand, within yahG at 338.188 kb on + strand, within yahG at 338.196 kb on - strand, within yahG at 338.196 kb on - strand, within yahG at 338.231 kb on + strand, within yahG at 338.237 kb on - strand, within yahG at 338.266 kb on + strand, within yahG at 338.365 kb on + strand, within yahG at 338.365 kb on + strand, within yahG at 338.424 kb on - strand, within yahG at 338.429 kb on - strand, within yahG at 338.551 kb on - strand, within yahG at 338.656 kb on - strand, within yahG at 338.902 kb on + strand at 338.902 kb on + strand at 339.006 kb on - strand at 339.184 kb on - strand at 339.195 kb on + strand at 339.195 kb on + strand at 339.203 kb on - strand at 339.203 kb on - strand at 339.378 kb on - strand at 339.390 kb on - strand at 339.390 kb on - strand at 339.411 kb on - strand at 339.431 kb on + strand at 339.444 kb on - strand at 339.565 kb on + strand, within yahI at 339.565 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.630 kb on + strand, within yahI at 339.741 kb on - strand, within yahI at 339.798 kb on + strand, within yahI at 339.806 kb on - strand, within yahI
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 336,554 - yahF b0320 0.36 +3.9 337,024 - yahF b0320 0.66 -0.8 337,163 - yahF b0320 0.75 +0.5 337,169 + yahF b0320 0.75 +0.2 337,169 + yahF b0320 0.75 -2.0 337,236 - yahF b0320 0.80 -0.0 337,603 + -1.3 337,603 + -0.5 337,763 + yahG b0321 0.15 -0.2 337,763 + yahG b0321 0.15 -0.4 337,821 + yahG b0321 0.19 -0.8 337,829 - yahG b0321 0.20 +0.5 337,974 - yahG b0321 0.30 +1.6 337,974 - yahG b0321 0.30 -0.3 338,157 + yahG b0321 0.43 +0.5 338,157 + yahG b0321 0.43 -1.9 338,178 + yahG b0321 0.44 -0.8 338,183 + yahG b0321 0.45 -1.0 338,183 + yahG b0321 0.45 -0.1 338,188 + yahG b0321 0.45 -0.1 338,196 - yahG b0321 0.46 +0.9 338,196 - yahG b0321 0.46 -0.5 338,231 + yahG b0321 0.48 -1.6 338,237 - yahG b0321 0.48 +0.6 338,266 + yahG b0321 0.51 -0.7 338,365 + yahG b0321 0.58 -1.0 338,365 + yahG b0321 0.58 -2.0 338,424 - yahG b0321 0.62 +0.8 338,429 - yahG b0321 0.62 +0.6 338,551 - yahG b0321 0.71 +0.6 338,656 - yahG b0321 0.78 +1.3 338,902 + -1.0 338,902 + -1.0 339,006 - -0.0 339,184 - +0.8 339,195 + -0.4 339,195 + +0.6 339,203 - +1.6 339,203 - +2.0 339,378 - -3.0 339,390 - -1.5 339,390 - +3.3 339,411 - +0.7 339,431 + -1.7 339,444 - +1.6 339,565 + yahI b0323 0.19 -1.3 339,565 + yahI b0323 0.19 -1.3 339,630 + yahI b0323 0.25 -1.9 339,630 + yahI b0323 0.25 -0.9 339,741 - yahI b0323 0.37 +1.0 339,798 + yahI b0323 0.43 -0.1 339,806 - yahI b0323 0.44 -0.5
Or see this region's nucleotide sequence