Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafK and yafQ are separated by 155 nucleotides yafQ and dinJ are separated by 2 nucleotides dinJ and yafL are separated by 209 nucleotides yafL and yafM are separated by 175 nucleotides yafM and lfhA are separated by 223 nucleotides
b0224: yafK - hypothetical protein (NCBI), at 245,065 to 245,805
yafK
b0225: yafQ - predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI), at 245,961 to 246,239
yafQ
b0226: dinJ - predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI), at 246,242 to 246,502
dinJ
b0227: yafL - predicted lipoprotein and C40 family peptidase (NCBI), at 246,712 to 247,461
yafL
b0228: yafM - hypothetical protein (NCBI), at 247,637 to 248,134
yafM
b0229: lfhA - flagellar biosynthesis (VIMSS), at 248,358 to 250,070
lfhA
Position (kb) 246
247
248 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 245.735 kb on - strand at 245.743 kb on - strand at 245.743 kb on - strand at 245.837 kb on + strand at 245.928 kb on - strand at 245.928 kb on - strand at 245.964 kb on + strand at 245.964 kb on + strand at 245.971 kb on - strand at 246.048 kb on + strand, within yafQ at 246.048 kb on + strand, within yafQ at 246.048 kb on + strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.086 kb on - strand, within yafQ at 246.097 kb on - strand, within yafQ at 246.294 kb on - strand, within dinJ at 246.295 kb on - strand, within dinJ at 246.304 kb on - strand, within dinJ at 246.330 kb on - strand, within dinJ at 246.338 kb on - strand, within dinJ at 246.338 kb on - strand, within dinJ at 246.411 kb on + strand, within dinJ at 246.411 kb on + strand, within dinJ at 246.742 kb on - strand at 246.821 kb on - strand, within yafL at 246.854 kb on + strand, within yafL at 246.859 kb on + strand, within yafL at 247.129 kb on - strand, within yafL at 247.129 kb on - strand, within yafL at 247.227 kb on - strand, within yafL at 247.227 kb on - strand, within yafL at 247.288 kb on + strand, within yafL at 247.312 kb on - strand, within yafL at 247.409 kb on - strand at 247.493 kb on + strand at 247.502 kb on - strand at 247.729 kb on + strand, within yafM at 247.786 kb on - strand, within yafM at 247.786 kb on - strand, within yafM at 247.797 kb on - strand, within yafM at 247.824 kb on - strand, within yafM at 247.831 kb on - strand, within yafM at 248.035 kb on + strand, within yafM at 248.067 kb on + strand, within yafM at 248.185 kb on - strand at 248.185 kb on - strand at 248.336 kb on + strand at 248.337 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 245,735 - +0.5 245,743 - +1.8 245,743 - +0.8 245,837 + +1.2 245,928 - -0.1 245,928 - -0.6 245,964 + -2.9 245,964 + -0.4 245,971 - -0.2 246,048 + yafQ b0225 0.31 +1.6 246,048 + yafQ b0225 0.31 -0.7 246,048 + yafQ b0225 0.31 +2.5 246,086 - yafQ b0225 0.45 +3.5 246,086 - yafQ b0225 0.45 -1.2 246,086 - yafQ b0225 0.45 -0.4 246,086 - yafQ b0225 0.45 +0.4 246,097 - yafQ b0225 0.49 -0.5 246,294 - dinJ b0226 0.20 +0.4 246,295 - dinJ b0226 0.20 -1.4 246,304 - dinJ b0226 0.24 +0.2 246,330 - dinJ b0226 0.34 -0.4 246,338 - dinJ b0226 0.37 +0.4 246,338 - dinJ b0226 0.37 -2.1 246,411 + dinJ b0226 0.65 -0.6 246,411 + dinJ b0226 0.65 -0.6 246,742 - +0.5 246,821 - yafL b0227 0.15 -2.4 246,854 + yafL b0227 0.19 -0.3 246,859 + yafL b0227 0.20 -1.2 247,129 - yafL b0227 0.56 -1.3 247,129 - yafL b0227 0.56 -0.6 247,227 - yafL b0227 0.69 +2.6 247,227 - yafL b0227 0.69 +0.4 247,288 + yafL b0227 0.77 -0.4 247,312 - yafL b0227 0.80 -0.1 247,409 - -0.4 247,493 + -1.2 247,502 - +0.4 247,729 + yafM b0228 0.18 -1.8 247,786 - yafM b0228 0.30 -1.6 247,786 - yafM b0228 0.30 +0.9 247,797 - yafM b0228 0.32 +2.6 247,824 - yafM b0228 0.38 +1.0 247,831 - yafM b0228 0.39 +0.9 248,035 + yafM b0228 0.80 +0.6 248,067 + yafM b0228 0.86 +0.9 248,185 - +1.3 248,185 - +0.4 248,336 + -2.9 248,337 - +0.5
Or see this region's nucleotide sequence