Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fhuB and hemL are separated by 157 nucleotides hemL and clcA are separated by 224 nucleotides
b0153: fhuB - fused iron-hydroxamate transporter subunits of ABC superfamily: membrane components (NCBI), at 171,462 to 173,444
fhuB
b0154: hemL - glutamate-1-semialdehyde aminotransferase (NCBI), at 173,602 to 174,882
hemL
b0155: clcA - chloride channel protein (NCBI), at 175,107 to 176,528
clcA
Position (kb) 173
174
175 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 172.687 kb on + strand, within fhuB at 172.695 kb on - strand, within fhuB at 172.723 kb on + strand, within fhuB at 172.731 kb on - strand, within fhuB at 172.734 kb on + strand, within fhuB at 172.755 kb on + strand, within fhuB at 172.767 kb on + strand, within fhuB at 172.767 kb on + strand, within fhuB at 172.823 kb on - strand, within fhuB at 172.823 kb on - strand, within fhuB at 172.922 kb on - strand, within fhuB at 172.930 kb on + strand, within fhuB at 172.946 kb on - strand, within fhuB at 172.946 kb on - strand, within fhuB at 173.030 kb on + strand, within fhuB at 173.122 kb on - strand, within fhuB at 173.122 kb on - strand, within fhuB at 173.126 kb on - strand, within fhuB at 173.209 kb on + strand, within fhuB at 173.232 kb on - strand, within fhuB at 173.241 kb on + strand, within fhuB at 173.280 kb on + strand at 173.280 kb on + strand at 173.288 kb on - strand at 173.288 kb on - strand at 173.329 kb on + strand at 173.378 kb on - strand at 173.392 kb on + strand at 173.401 kb on - strand at 173.401 kb on - strand at 173.463 kb on + strand at 173.463 kb on - strand at 173.468 kb on + strand at 173.478 kb on + strand at 173.600 kb on + strand at 173.600 kb on - strand at 174.690 kb on - strand, within hemL at 174.958 kb on + strand at 174.968 kb on + strand at 174.968 kb on + strand at 174.993 kb on - strand at 174.993 kb on - strand at 175.096 kb on - strand at 175.167 kb on - strand at 175.227 kb on + strand at 175.273 kb on - strand, within clcA at 175.273 kb on - strand, within clcA at 175.273 kb on - strand, within clcA at 175.284 kb on + strand, within clcA at 175.308 kb on + strand, within clcA at 175.316 kb on - strand, within clcA at 175.316 kb on - strand, within clcA at 175.345 kb on - strand, within clcA at 175.345 kb on - strand, within clcA at 175.483 kb on - strand, within clcA at 175.491 kb on - strand, within clcA at 175.531 kb on - strand, within clcA at 175.536 kb on + strand, within clcA at 175.536 kb on + strand, within clcA at 175.536 kb on + strand, within clcA at 175.569 kb on + strand, within clcA at 175.569 kb on + strand, within clcA at 175.579 kb on - strand, within clcA at 175.582 kb on + strand, within clcA at 175.583 kb on + strand, within clcA at 175.607 kb on + strand, within clcA at 175.627 kb on + strand, within clcA at 175.635 kb on - strand, within clcA at 175.648 kb on + strand, within clcA at 175.648 kb on + strand, within clcA at 175.758 kb on + strand, within clcA at 175.762 kb on + strand, within clcA at 175.781 kb on + strand, within clcA at 175.818 kb on - strand, within clcA at 175.850 kb on + strand, within clcA at 175.851 kb on + strand, within clcA at 175.851 kb on + strand, within clcA at 175.864 kb on + strand, within clcA
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 172,687 + fhuB b0153 0.62 +3.4 172,695 - fhuB b0153 0.62 -1.8 172,723 + fhuB b0153 0.64 +0.6 172,731 - fhuB b0153 0.64 +1.7 172,734 + fhuB b0153 0.64 +1.3 172,755 + fhuB b0153 0.65 +0.1 172,767 + fhuB b0153 0.66 +1.1 172,767 + fhuB b0153 0.66 +1.6 172,823 - fhuB b0153 0.69 -0.1 172,823 - fhuB b0153 0.69 -2.0 172,922 - fhuB b0153 0.74 -0.5 172,930 + fhuB b0153 0.74 +1.0 172,946 - fhuB b0153 0.75 +0.8 172,946 - fhuB b0153 0.75 -1.1 173,030 + fhuB b0153 0.79 -2.3 173,122 - fhuB b0153 0.84 +0.9 173,122 - fhuB b0153 0.84 -1.7 173,126 - fhuB b0153 0.84 -2.0 173,209 + fhuB b0153 0.88 -0.1 173,232 - fhuB b0153 0.89 +1.5 173,241 + fhuB b0153 0.90 +2.8 173,280 + -1.6 173,280 + +0.3 173,288 - -2.7 173,288 - +0.1 173,329 + -0.6 173,378 - -0.4 173,392 + +0.6 173,401 - +0.8 173,401 - -1.8 173,463 + +2.3 173,463 - -1.8 173,468 + +2.1 173,478 + +0.7 173,600 + +0.1 173,600 - +0.6 174,690 - hemL b0154 0.85 +1.4 174,958 + +2.6 174,968 + -1.0 174,968 + -0.3 174,993 - +1.6 174,993 - +0.4 175,096 - -1.8 175,167 - +0.9 175,227 + +1.4 175,273 - clcA b0155 0.12 -1.4 175,273 - clcA b0155 0.12 +1.4 175,273 - clcA b0155 0.12 +1.9 175,284 + clcA b0155 0.12 -1.0 175,308 + clcA b0155 0.14 +1.5 175,316 - clcA b0155 0.15 +0.2 175,316 - clcA b0155 0.15 -0.1 175,345 - clcA b0155 0.17 -0.7 175,345 - clcA b0155 0.17 -1.0 175,483 - clcA b0155 0.26 -1.2 175,491 - clcA b0155 0.27 +1.0 175,531 - clcA b0155 0.30 +1.0 175,536 + clcA b0155 0.30 -1.4 175,536 + clcA b0155 0.30 +2.4 175,536 + clcA b0155 0.30 +0.4 175,569 + clcA b0155 0.32 -1.6 175,569 + clcA b0155 0.32 +1.1 175,579 - clcA b0155 0.33 +2.2 175,582 + clcA b0155 0.33 +0.2 175,583 + clcA b0155 0.33 -2.2 175,607 + clcA b0155 0.35 +0.5 175,627 + clcA b0155 0.37 +0.1 175,635 - clcA b0155 0.37 +1.6 175,648 + clcA b0155 0.38 +0.3 175,648 + clcA b0155 0.38 -1.4 175,758 + clcA b0155 0.46 -0.4 175,762 + clcA b0155 0.46 +0.4 175,781 + clcA b0155 0.47 -0.8 175,818 - clcA b0155 0.50 +1.8 175,850 + clcA b0155 0.52 -2.4 175,851 + clcA b0155 0.52 +0.5 175,851 + clcA b0155 0.52 +0.4 175,864 + clcA b0155 0.53 +1.0
Or see this region's nucleotide sequence