Experiment: Benzalkonium Chloride 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaaU and kefF are separated by 107 nucleotides kefF and kefC overlap by 8 nucleotides
b0045: yaaU - predicted transporter (NCBI), at 45,807 to 47,138
yaaU
b0046: kefF - glutathione-regulated potassium-efflux system ancillary protein (NCBI), at 47,246 to 47,776
kefF
b0047: kefC - glutathione-regulated potassium-efflux system protein (NCBI), at 47,769 to 49,631
kefC
Position (kb) 47
48 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 46.284 kb on - strand, within yaaU at 46.329 kb on + strand, within yaaU at 46.329 kb on + strand, within yaaU at 46.463 kb on + strand, within yaaU at 46.463 kb on + strand, within yaaU at 46.564 kb on + strand, within yaaU at 46.564 kb on + strand, within yaaU at 46.575 kb on + strand, within yaaU at 46.707 kb on - strand, within yaaU at 46.707 kb on - strand, within yaaU at 46.800 kb on + strand, within yaaU at 46.920 kb on - strand, within yaaU at 47.003 kb on + strand, within yaaU at 47.067 kb on + strand at 47.097 kb on - strand at 47.097 kb on - strand at 47.113 kb on - strand at 47.113 kb on - strand at 47.202 kb on - strand at 47.225 kb on + strand at 47.225 kb on + strand at 47.247 kb on - strand at 47.269 kb on + strand at 47.359 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.402 kb on - strand, within kefF at 47.412 kb on - strand, within kefF at 47.446 kb on + strand, within kefF at 47.454 kb on + strand, within kefF at 47.503 kb on + strand, within kefF at 47.504 kb on + strand, within kefF at 47.504 kb on + strand, within kefF at 47.639 kb on + strand, within kefF at 47.651 kb on + strand, within kefF at 47.675 kb on + strand, within kefF at 47.681 kb on + strand, within kefF at 47.681 kb on + strand, within kefF at 47.730 kb on + strand at 47.762 kb on + strand at 47.774 kb on - strand at 47.784 kb on - strand at 47.823 kb on + strand at 47.823 kb on + strand at 47.832 kb on + strand at 47.832 kb on + strand at 47.836 kb on + strand at 47.848 kb on + strand at 47.859 kb on + strand at 47.921 kb on + strand at 47.948 kb on + strand at 47.948 kb on + strand at 47.958 kb on + strand, within kefC at 47.958 kb on + strand, within kefC at 47.977 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.031 kb on + strand, within kefC at 48.227 kb on + strand, within kefC at 48.227 kb on - strand, within kefC at 48.228 kb on + strand, within kefC at 48.235 kb on - strand, within kefC at 48.253 kb on - strand, within kefC at 48.348 kb on + strand, within kefC at 48.394 kb on + strand, within kefC at 48.408 kb on + strand, within kefC at 48.408 kb on + strand, within kefC at 48.569 kb on + strand, within kefC at 48.569 kb on + strand, within kefC at 48.614 kb on + strand, within kefC at 48.760 kb on + strand, within kefC at 48.760 kb on + strand, within kefC
Per-strain Table
Position Strand Gene LocusTag Fraction Benzalkonium Chloride 0.004 mg/ml remove 46,284 - yaaU b0045 0.36 +0.5 46,329 + yaaU b0045 0.39 +0.5 46,329 + yaaU b0045 0.39 -1.6 46,463 + yaaU b0045 0.49 +0.8 46,463 + yaaU b0045 0.49 +1.4 46,564 + yaaU b0045 0.57 +0.2 46,564 + yaaU b0045 0.57 -2.0 46,575 + yaaU b0045 0.58 -1.4 46,707 - yaaU b0045 0.68 -2.3 46,707 - yaaU b0045 0.68 +0.0 46,800 + yaaU b0045 0.75 +4.3 46,920 - yaaU b0045 0.84 -0.4 47,003 + yaaU b0045 0.90 +3.1 47,067 + +0.6 47,097 - -3.4 47,097 - +2.9 47,113 - -1.4 47,113 - +0.4 47,202 - +1.4 47,225 + -1.0 47,225 + +0.0 47,247 - +1.9 47,269 + -0.9 47,359 - kefF b0046 0.21 +1.0 47,364 - kefF b0046 0.22 +2.4 47,364 - kefF b0046 0.22 +0.7 47,364 - kefF b0046 0.22 +0.7 47,364 - kefF b0046 0.22 +0.4 47,402 - kefF b0046 0.29 -0.0 47,412 - kefF b0046 0.31 +0.8 47,446 + kefF b0046 0.38 -1.7 47,454 + kefF b0046 0.39 -0.7 47,503 + kefF b0046 0.48 +4.0 47,504 + kefF b0046 0.49 -1.8 47,504 + kefF b0046 0.49 -0.3 47,639 + kefF b0046 0.74 -0.6 47,651 + kefF b0046 0.76 -2.5 47,675 + kefF b0046 0.81 -0.4 47,681 + kefF b0046 0.82 -0.2 47,681 + kefF b0046 0.82 -1.4 47,730 + -0.7 47,762 + +1.2 47,774 - +0.3 47,784 - -0.6 47,823 + -2.0 47,823 + -2.0 47,832 + -0.6 47,832 + +5.8 47,836 + +1.1 47,848 + -0.1 47,859 + +0.5 47,921 + -1.6 47,948 + +0.0 47,948 + +0.4 47,958 + kefC b0047 0.10 +1.0 47,958 + kefC b0047 0.10 +1.8 47,977 - kefC b0047 0.11 -0.3 48,009 - kefC b0047 0.13 +1.8 48,009 - kefC b0047 0.13 -1.4 48,009 - kefC b0047 0.13 +0.4 48,031 + kefC b0047 0.14 -0.2 48,227 + kefC b0047 0.25 -1.2 48,227 - kefC b0047 0.25 +2.0 48,228 + kefC b0047 0.25 -3.0 48,235 - kefC b0047 0.25 +0.5 48,253 - kefC b0047 0.26 +0.4 48,348 + kefC b0047 0.31 +0.1 48,394 + kefC b0047 0.34 +0.5 48,408 + kefC b0047 0.34 -2.1 48,408 + kefC b0047 0.34 +1.4 48,569 + kefC b0047 0.43 +0.0 48,569 + kefC b0047 0.43 +2.2 48,614 + kefC b0047 0.45 +1.7 48,760 + kefC b0047 0.53 +0.6 48,760 + kefC b0047 0.53 -1.0
Or see this region's nucleotide sequence