Experiment: butyrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS29025 and ABID97_RS29030 are separated by 21 nucleotides ABID97_RS29030 and ABID97_RS29035 are separated by 26 nucleotides ABID97_RS29035 and ABID97_RS29040 overlap by 4 nucleotides ABID97_RS29040 and ABID97_RS29045 are separated by 168 nucleotides
ABID97_RS29025: ABID97_RS29025 - 3-isopropylmalate dehydratase large subunit, at 722,891 to 724,312
_RS29025
ABID97_RS29030: ABID97_RS29030 - 3-isopropylmalate dehydratase small subunit, at 724,334 to 724,936
_RS29030
ABID97_RS29035: ABID97_RS29035 - 3-isopropylmalate dehydrogenase, at 724,963 to 726,042
_RS29035
ABID97_RS29040: ABID97_RS29040 - IclR family transcriptional regulator, at 726,039 to 726,845
_RS29040
ABID97_RS29045: ABID97_RS29045 - tripartite tricarboxylate transporter substrate binding protein, at 727,014 to 728,030
_RS29045
Position (kb) 724
725
726
727 Strain fitness (log2 ratio) -2
-1
0
1 at 724.454 kb on + strand, within ABID97_RS29030 at 724.970 kb on + strand at 724.971 kb on - strand at 725.375 kb on - strand, within ABID97_RS29035 at 725.782 kb on + strand, within ABID97_RS29035 at 725.782 kb on + strand, within ABID97_RS29035 at 725.782 kb on + strand, within ABID97_RS29035 at 726.040 kb on + strand at 726.463 kb on + strand, within ABID97_RS29040 at 726.464 kb on - strand, within ABID97_RS29040 at 726.933 kb on + strand at 726.940 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction butyrate (C) remove 724,454 + ABID97_RS29030 0.20 -2.0 724,970 + +0.3 724,971 - -0.4 725,375 - ABID97_RS29035 0.38 -1.7 725,782 + ABID97_RS29035 0.76 -1.1 725,782 + ABID97_RS29035 0.76 -0.9 725,782 + ABID97_RS29035 0.76 +0.5 726,040 + -0.7 726,463 + ABID97_RS29040 0.53 -0.2 726,464 - ABID97_RS29040 0.53 -0.6 726,933 + -0.4 726,940 + +1.3
Or see this region's nucleotide sequence