Experiment: L-Malic (C) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_03719 and PFR28_03720 are separated by 3 nucleotides PFR28_03720 and PFR28_03721 are separated by 267 nucleotides PFR28_03721 and PFR28_03722 overlap by 4 nucleotides
PFR28_03719: PFR28_03719 - Poly(A) polymerase I, at 4,286,855 to 4,288,252
_03719
PFR28_03720: PFR28_03720 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 4,288,256 to 4,288,741
_03720
PFR28_03721: PFR28_03721 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 4,289,009 to 4,289,809
_03721
PFR28_03722: PFR28_03722 - Pantothenate synthetase, at 4,289,806 to 4,290,666
_03722
Position (kb)
4289
4290 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4288.268 kb on + strand at 4288.268 kb on + strand at 4288.268 kb on + strand at 4288.268 kb on + strand at 4288.268 kb on + strand at 4288.268 kb on + strand at 4288.268 kb on + strand at 4288.268 kb on + strand at 4288.268 kb on + strand at 4288.822 kb on + strand at 4288.823 kb on - strand at 4288.900 kb on + strand at 4288.900 kb on + strand at 4288.900 kb on + strand at 4288.901 kb on - strand at 4288.901 kb on - strand at 4288.901 kb on - strand at 4288.902 kb on + strand at 4288.902 kb on + strand at 4288.903 kb on - strand at 4288.903 kb on - strand at 4288.903 kb on - strand at 4288.903 kb on - strand at 4288.952 kb on + strand at 4288.952 kb on + strand at 4288.952 kb on + strand at 4288.952 kb on + strand at 4288.953 kb on - strand at 4288.953 kb on - strand at 4288.953 kb on - strand at 4288.953 kb on - strand at 4288.953 kb on - strand at 4288.953 kb on - strand at 4288.967 kb on + strand at 4288.967 kb on + strand at 4288.967 kb on + strand at 4288.968 kb on - strand at 4288.983 kb on - strand at 4289.017 kb on + strand at 4289.017 kb on + strand at 4289.017 kb on + strand at 4289.017 kb on + strand at 4289.018 kb on - strand at 4289.018 kb on - strand at 4289.020 kb on + strand at 4289.081 kb on + strand at 4289.081 kb on + strand at 4289.081 kb on + strand at 4289.081 kb on + strand at 4289.107 kb on + strand, within PFR28_03721 at 4289.130 kb on + strand, within PFR28_03721 at 4289.207 kb on + strand, within PFR28_03721 at 4289.207 kb on + strand, within PFR28_03721 at 4289.207 kb on + strand, within PFR28_03721 at 4289.207 kb on + strand, within PFR28_03721 at 4289.207 kb on + strand, within PFR28_03721 at 4289.207 kb on + strand, within PFR28_03721 at 4289.207 kb on + strand, within PFR28_03721 at 4289.207 kb on + strand, within PFR28_03721 at 4289.208 kb on - strand, within PFR28_03721 at 4289.276 kb on + strand, within PFR28_03721 at 4289.276 kb on + strand, within PFR28_03721 at 4289.276 kb on + strand, within PFR28_03721 at 4289.276 kb on + strand, within PFR28_03721 at 4289.277 kb on - strand, within PFR28_03721 at 4289.277 kb on - strand, within PFR28_03721 at 4289.646 kb on + strand, within PFR28_03721 at 4289.646 kb on + strand, within PFR28_03721 at 4289.646 kb on + strand, within PFR28_03721 at 4289.647 kb on - strand, within PFR28_03721 at 4289.647 kb on - strand, within PFR28_03721 at 4289.750 kb on + strand at 4289.750 kb on + strand at 4289.750 kb on + strand at 4289.751 kb on - strand at 4289.751 kb on - strand at 4289.826 kb on - strand at 4290.785 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) 10 mM remove 4,288,268 + -0.8 4,288,268 + -3.2 4,288,268 + -2.6 4,288,268 + -0.1 4,288,268 + +1.0 4,288,268 + -0.9 4,288,268 + -1.0 4,288,268 + -2.3 4,288,268 + -2.8 4,288,822 + -1.3 4,288,823 - -0.1 4,288,900 + +0.6 4,288,900 + +0.8 4,288,900 + +2.7 4,288,901 - +0.6 4,288,901 - +0.6 4,288,901 - -0.8 4,288,902 + +1.0 4,288,902 + -1.8 4,288,903 - -1.6 4,288,903 - -1.5 4,288,903 - -0.5 4,288,903 - -2.0 4,288,952 + +0.6 4,288,952 + +0.0 4,288,952 + -0.8 4,288,952 + +0.9 4,288,953 - -0.4 4,288,953 - +0.1 4,288,953 - +0.5 4,288,953 - -0.1 4,288,953 - +1.8 4,288,953 - -1.0 4,288,967 + +2.1 4,288,967 + +1.3 4,288,967 + -0.4 4,288,968 - +0.4 4,288,983 - +0.8 4,289,017 + -1.1 4,289,017 + +0.5 4,289,017 + -2.3 4,289,017 + -3.0 4,289,018 - -1.8 4,289,018 - -2.8 4,289,020 + -0.7 4,289,081 + -1.7 4,289,081 + -3.0 4,289,081 + +0.5 4,289,081 + +0.9 4,289,107 + PFR28_03721 0.12 -1.5 4,289,130 + PFR28_03721 0.15 -3.3 4,289,207 + PFR28_03721 0.25 -0.8 4,289,207 + PFR28_03721 0.25 +0.0 4,289,207 + PFR28_03721 0.25 +0.4 4,289,207 + PFR28_03721 0.25 -1.6 4,289,207 + PFR28_03721 0.25 -0.9 4,289,207 + PFR28_03721 0.25 -2.6 4,289,207 + PFR28_03721 0.25 -2.8 4,289,207 + PFR28_03721 0.25 -3.0 4,289,208 - PFR28_03721 0.25 -2.6 4,289,276 + PFR28_03721 0.33 -2.4 4,289,276 + PFR28_03721 0.33 -1.4 4,289,276 + PFR28_03721 0.33 -1.3 4,289,276 + PFR28_03721 0.33 -1.5 4,289,277 - PFR28_03721 0.33 -1.8 4,289,277 - PFR28_03721 0.33 -0.2 4,289,646 + PFR28_03721 0.80 +0.1 4,289,646 + PFR28_03721 0.80 -3.7 4,289,646 + PFR28_03721 0.80 -2.7 4,289,647 - PFR28_03721 0.80 -1.8 4,289,647 - PFR28_03721 0.80 -2.3 4,289,750 + +0.4 4,289,750 + +0.6 4,289,750 + -2.3 4,289,751 - -3.0 4,289,751 - -2.9 4,289,826 - -2.4 4,290,785 - -0.3
Or see this region's nucleotide sequence