Experiment: L-Malic (C) 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFR28_00269 and PFR28_00270 are separated by 19 nucleotides PFR28_00270 and PFR28_00271 are separated by 189 nucleotides
PFR28_00269: PFR28_00269 - 4-hydroxy-tetrahydrodipicolinate synthase, at 297,795 to 298,682
_00269
PFR28_00270: PFR28_00270 - 4-hydroxy-3-prenylphenylpyruvate oxygenase/4-hydroxy-3-prenylbenzoate synthase, at 298,702 to 299,484
_00270
PFR28_00271: PFR28_00271 - hypothetical protein, at 299,674 to 300,201
_00271
Position (kb) 299
300
301 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 298.745 kb on + strand at 298.745 kb on + strand at 298.745 kb on + strand at 298.746 kb on - strand at 298.748 kb on + strand at 298.748 kb on + strand at 298.748 kb on + strand at 298.749 kb on - strand at 298.749 kb on - strand at 299.045 kb on + strand, within PFR28_00270 at 299.045 kb on + strand, within PFR28_00270 at 299.159 kb on + strand, within PFR28_00270 at 299.288 kb on + strand, within PFR28_00270 at 299.288 kb on + strand, within PFR28_00270 at 299.288 kb on + strand, within PFR28_00270 at 299.289 kb on - strand, within PFR28_00270 at 299.289 kb on - strand, within PFR28_00270 at 299.289 kb on - strand, within PFR28_00270 at 299.289 kb on - strand, within PFR28_00270 at 299.289 kb on - strand, within PFR28_00270 at 299.289 kb on - strand, within PFR28_00270 at 299.289 kb on - strand, within PFR28_00270 at 299.291 kb on + strand, within PFR28_00270 at 299.291 kb on + strand, within PFR28_00270 at 299.291 kb on + strand, within PFR28_00270 at 299.291 kb on + strand, within PFR28_00270 at 299.292 kb on - strand, within PFR28_00270 at 299.292 kb on - strand, within PFR28_00270 at 299.292 kb on - strand, within PFR28_00270 at 299.292 kb on - strand, within PFR28_00270 at 299.292 kb on - strand, within PFR28_00270 at 299.292 kb on - strand, within PFR28_00270 at 299.292 kb on - strand, within PFR28_00270 at 299.292 kb on - strand, within PFR28_00270 at 299.392 kb on + strand, within PFR28_00270 at 299.403 kb on + strand, within PFR28_00270 at 299.403 kb on + strand, within PFR28_00270 at 299.403 kb on + strand, within PFR28_00270 at 299.404 kb on - strand, within PFR28_00270 at 299.407 kb on - strand at 299.484 kb on + strand at 299.634 kb on + strand at 299.634 kb on + strand at 299.634 kb on + strand at 299.634 kb on + strand at 299.634 kb on + strand at 299.634 kb on + strand at 299.634 kb on + strand at 299.634 kb on + strand at 299.634 kb on + strand at 299.634 kb on + strand at 299.634 kb on + strand at 299.635 kb on - strand at 299.635 kb on - strand at 299.635 kb on - strand at 299.635 kb on - strand at 299.635 kb on - strand at 299.635 kb on - strand at 299.635 kb on - strand at 299.635 kb on - strand at 299.635 kb on - strand at 299.719 kb on + strand at 299.719 kb on + strand at 299.719 kb on + strand at 299.720 kb on - strand at 299.720 kb on - strand at 299.842 kb on + strand, within PFR28_00271 at 299.842 kb on + strand, within PFR28_00271 at 299.842 kb on + strand, within PFR28_00271 at 299.842 kb on + strand, within PFR28_00271 at 299.843 kb on - strand, within PFR28_00271 at 299.843 kb on - strand, within PFR28_00271 at 299.843 kb on - strand, within PFR28_00271 at 299.843 kb on - strand, within PFR28_00271 at 299.843 kb on - strand, within PFR28_00271 at 299.843 kb on - strand, within PFR28_00271 at 299.843 kb on - strand, within PFR28_00271 at 299.843 kb on - strand, within PFR28_00271 at 299.843 kb on - strand, within PFR28_00271 at 299.843 kb on - strand, within PFR28_00271 at 300.200 kb on - strand at 300.274 kb on - strand at 300.359 kb on - strand at 300.359 kb on - strand at 300.420 kb on - strand at 300.425 kb on + strand at 300.425 kb on + strand at 300.426 kb on - strand at 300.426 kb on - strand at 300.426 kb on - strand at 300.467 kb on + strand at 300.522 kb on - strand at 300.522 kb on - strand at 300.612 kb on - strand at 300.627 kb on + strand at 300.693 kb on + strand at 300.693 kb on + strand at 300.693 kb on + strand at 300.694 kb on - strand at 300.694 kb on - strand at 300.694 kb on - strand at 300.694 kb on - strand at 300.694 kb on - strand at 300.694 kb on - strand at 300.694 kb on - strand at 300.712 kb on - strand at 300.729 kb on + strand at 300.729 kb on + strand at 300.729 kb on + strand at 300.730 kb on - strand at 300.730 kb on - strand at 300.730 kb on - strand at 300.811 kb on + strand at 300.812 kb on - strand at 300.812 kb on - strand at 300.815 kb on - strand at 300.815 kb on - strand at 300.832 kb on - strand at 301.047 kb on + strand at 301.063 kb on + strand at 301.064 kb on - strand at 301.110 kb on + strand at 301.140 kb on + strand at 301.140 kb on + strand at 301.140 kb on + strand at 301.160 kb on - strand at 301.179 kb on + strand at 301.180 kb on - strand at 301.182 kb on + strand at 301.183 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) 10 mM remove 298,745 + +0.8 298,745 + -0.2 298,745 + +0.8 298,746 - +0.5 298,748 + +0.5 298,748 + +0.2 298,748 + -0.2 298,749 - +0.5 298,749 - -0.1 299,045 + PFR28_00270 0.44 +0.8 299,045 + PFR28_00270 0.44 -1.3 299,159 + PFR28_00270 0.58 +0.6 299,288 + PFR28_00270 0.75 +0.7 299,288 + PFR28_00270 0.75 +1.0 299,288 + PFR28_00270 0.75 +0.6 299,289 - PFR28_00270 0.75 -1.1 299,289 - PFR28_00270 0.75 +0.6 299,289 - PFR28_00270 0.75 +1.0 299,289 - PFR28_00270 0.75 +0.8 299,289 - PFR28_00270 0.75 -0.4 299,289 - PFR28_00270 0.75 -0.1 299,289 - PFR28_00270 0.75 -0.5 299,291 + PFR28_00270 0.75 +1.7 299,291 + PFR28_00270 0.75 -0.2 299,291 + PFR28_00270 0.75 -0.4 299,291 + PFR28_00270 0.75 +0.0 299,292 - PFR28_00270 0.75 -0.1 299,292 - PFR28_00270 0.75 -0.4 299,292 - PFR28_00270 0.75 +0.7 299,292 - PFR28_00270 0.75 -0.4 299,292 - PFR28_00270 0.75 -0.2 299,292 - PFR28_00270 0.75 -0.3 299,292 - PFR28_00270 0.75 -1.1 299,292 - PFR28_00270 0.75 -1.1 299,392 + PFR28_00270 0.88 -0.8 299,403 + PFR28_00270 0.90 -0.1 299,403 + PFR28_00270 0.90 -0.3 299,403 + PFR28_00270 0.90 -0.9 299,404 - PFR28_00270 0.90 -0.7 299,407 - -0.2 299,484 + +0.1 299,634 + +0.4 299,634 + -2.6 299,634 + -0.3 299,634 + -2.5 299,634 + +1.4 299,634 + -2.4 299,634 + +0.5 299,634 + +0.7 299,634 + +1.0 299,634 + +1.5 299,634 + +0.8 299,635 - -1.7 299,635 - -0.4 299,635 - -2.9 299,635 - -2.3 299,635 - -3.5 299,635 - -1.3 299,635 - -1.1 299,635 - -3.3 299,635 - -0.1 299,719 + +1.6 299,719 + +0.1 299,719 + +0.5 299,720 - -3.7 299,720 - +1.2 299,842 + PFR28_00271 0.32 -1.6 299,842 + PFR28_00271 0.32 +1.0 299,842 + PFR28_00271 0.32 -1.1 299,842 + PFR28_00271 0.32 -0.3 299,843 - PFR28_00271 0.32 -0.7 299,843 - PFR28_00271 0.32 +1.5 299,843 - PFR28_00271 0.32 -0.5 299,843 - PFR28_00271 0.32 -0.9 299,843 - PFR28_00271 0.32 -0.3 299,843 - PFR28_00271 0.32 -1.0 299,843 - PFR28_00271 0.32 -1.2 299,843 - PFR28_00271 0.32 -1.7 299,843 - PFR28_00271 0.32 +1.5 299,843 - PFR28_00271 0.32 -1.4 300,200 - -0.5 300,274 - -1.0 300,359 - +1.8 300,359 - +0.6 300,420 - -0.1 300,425 + +1.5 300,425 + -0.1 300,426 - -0.2 300,426 - +0.5 300,426 - +0.2 300,467 + -0.8 300,522 - +2.2 300,522 - +0.4 300,612 - +0.4 300,627 + +0.1 300,693 + -0.3 300,693 + +0.3 300,693 + +0.7 300,694 - +0.0 300,694 - +0.6 300,694 - +0.4 300,694 - +0.3 300,694 - -0.1 300,694 - +1.0 300,694 - -0.1 300,712 - +0.0 300,729 + -0.1 300,729 + +2.0 300,729 + +0.6 300,730 - +0.3 300,730 - -1.9 300,730 - -0.3 300,811 + -1.2 300,812 - -2.2 300,812 - +0.4 300,815 - +0.1 300,815 - +1.8 300,832 - +0.7 301,047 + +0.4 301,063 + +0.0 301,064 - +0.6 301,110 + +1.6 301,140 + +2.0 301,140 + +0.0 301,140 + +0.1 301,160 - -0.6 301,179 + -0.5 301,180 - +0.6 301,182 + -0.3 301,183 - +0.8
Or see this region's nucleotide sequence