Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF952 and GFF953 are separated by 63 nucleotides GFF953 and GFF954 are separated by 61 nucleotides GFF954 and GFF955 are separated by 24 nucleotides
GFF952 - Positive regulator of L-idonate catabolism, at 128,083 to 129,081
GFF952
GFF953 - L-idonate, D-gluconate, 5-keto-D-gluconate transporter, at 129,145 to 130,464
GFF953
GFF954 - 5-keto-D-gluconate 5-reductase (EC 1.1.1.69), at 130,526 to 131,290
GFF954
GFF955 - L-idonate 5-dehydrogenase (EC 1.1.1.264), at 131,315 to 132,346
GFF955
Position (kb)
129
130
131 Strain fitness (log2 ratio)
-1
0
1 at 128.206 kb on - strand, within GFF952 at 128.315 kb on - strand, within GFF952 at 128.392 kb on + strand, within GFF952 at 128.393 kb on - strand, within GFF952 at 128.421 kb on + strand, within GFF952 at 128.724 kb on + strand, within GFF952 at 128.729 kb on + strand, within GFF952 at 128.729 kb on + strand, within GFF952 at 128.729 kb on + strand, within GFF952 at 128.729 kb on + strand, within GFF952 at 128.729 kb on + strand, within GFF952 at 128.730 kb on - strand, within GFF952 at 128.775 kb on - strand, within GFF952 at 128.806 kb on - strand, within GFF952 at 128.913 kb on + strand, within GFF952 at 128.914 kb on - strand, within GFF952 at 128.933 kb on - strand, within GFF952 at 128.933 kb on - strand, within GFF952 at 128.941 kb on - strand, within GFF952 at 129.353 kb on - strand, within GFF953 at 129.413 kb on - strand, within GFF953 at 129.421 kb on - strand, within GFF953 at 129.451 kb on + strand, within GFF953 at 129.492 kb on + strand, within GFF953 at 129.492 kb on + strand, within GFF953 at 129.492 kb on + strand, within GFF953 at 129.493 kb on - strand, within GFF953 at 129.493 kb on - strand, within GFF953 at 129.494 kb on + strand, within GFF953 at 129.494 kb on + strand, within GFF953 at 129.495 kb on - strand, within GFF953 at 129.654 kb on + strand, within GFF953 at 129.663 kb on + strand, within GFF953 at 129.664 kb on - strand, within GFF953 at 129.743 kb on - strand, within GFF953 at 129.837 kb on + strand, within GFF953 at 129.928 kb on + strand, within GFF953 at 129.936 kb on - strand, within GFF953 at 130.052 kb on + strand, within GFF953 at 130.053 kb on - strand, within GFF953 at 130.195 kb on - strand, within GFF953 at 130.244 kb on - strand, within GFF953 at 130.478 kb on - strand at 130.753 kb on + strand, within GFF954 at 130.897 kb on + strand, within GFF954 at 131.053 kb on + strand, within GFF954 at 131.096 kb on - strand, within GFF954 at 131.117 kb on + strand, within GFF954 at 131.182 kb on + strand, within GFF954 at 131.224 kb on - strand at 131.293 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 128,206 - GFF952 0.12 -0.5 128,315 - GFF952 0.23 -0.1 128,392 + GFF952 0.31 +0.2 128,393 - GFF952 0.31 -1.4 128,421 + GFF952 0.34 +0.6 128,724 + GFF952 0.64 +0.0 128,729 + GFF952 0.65 +0.2 128,729 + GFF952 0.65 -0.1 128,729 + GFF952 0.65 +0.2 128,729 + GFF952 0.65 +0.1 128,729 + GFF952 0.65 +0.4 128,730 - GFF952 0.65 -0.3 128,775 - GFF952 0.69 +0.0 128,806 - GFF952 0.72 +0.3 128,913 + GFF952 0.83 -1.0 128,914 - GFF952 0.83 -0.2 128,933 - GFF952 0.85 -1.2 128,933 - GFF952 0.85 +0.2 128,941 - GFF952 0.86 +0.0 129,353 - GFF953 0.16 -1.2 129,413 - GFF953 0.20 -0.1 129,421 - GFF953 0.21 -0.1 129,451 + GFF953 0.23 +0.1 129,492 + GFF953 0.26 +0.5 129,492 + GFF953 0.26 -1.4 129,492 + GFF953 0.26 +0.2 129,493 - GFF953 0.26 +0.1 129,493 - GFF953 0.26 -0.2 129,494 + GFF953 0.26 +0.6 129,494 + GFF953 0.26 -0.8 129,495 - GFF953 0.27 +0.1 129,654 + GFF953 0.39 -0.2 129,663 + GFF953 0.39 -1.1 129,664 - GFF953 0.39 +0.3 129,743 - GFF953 0.45 +1.2 129,837 + GFF953 0.52 +0.3 129,928 + GFF953 0.59 -0.0 129,936 - GFF953 0.60 -1.1 130,052 + GFF953 0.69 +0.3 130,053 - GFF953 0.69 +0.0 130,195 - GFF953 0.80 -0.3 130,244 - GFF953 0.83 -0.2 130,478 - +1.6 130,753 + GFF954 0.30 +0.5 130,897 + GFF954 0.48 +0.4 131,053 + GFF954 0.69 +0.4 131,096 - GFF954 0.75 +0.4 131,117 + GFF954 0.77 -0.0 131,182 + GFF954 0.86 -0.2 131,224 - +0.7 131,293 + +0.8
Or see this region's nucleotide sequence