Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF721 and GFF722 are separated by 77 nucleotides GFF722 and GFF723 are separated by 5 nucleotides
GFF721 - HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217), at 77,524 to 78,333
GFF721
GFF722 - Pyruvate formate-lyase (EC 2.3.1.54), at 78,411 to 80,843
GFF722
GFF723 - Pyruvate formate-lyase activating enzyme (EC 1.97.1.4), at 80,849 to 81,748
GFF723
Position (kb) 78
79
80
81 Strain fitness (log2 ratio) -1
0
1
2 at 77.520 kb on - strand at 77.520 kb on - strand at 77.524 kb on - strand at 77.584 kb on + strand at 77.595 kb on - strand at 77.632 kb on - strand, within GFF721 at 77.668 kb on + strand, within GFF721 at 77.741 kb on - strand, within GFF721 at 77.820 kb on + strand, within GFF721 at 77.860 kb on + strand, within GFF721 at 77.861 kb on - strand, within GFF721 at 77.931 kb on - strand, within GFF721 at 77.964 kb on + strand, within GFF721 at 78.023 kb on + strand, within GFF721 at 78.024 kb on - strand, within GFF721 at 78.096 kb on + strand, within GFF721 at 78.205 kb on + strand, within GFF721 at 78.364 kb on - strand at 78.387 kb on + strand at 78.419 kb on + strand at 78.419 kb on + strand at 78.420 kb on - strand at 78.420 kb on - strand at 78.440 kb on + strand at 78.442 kb on + strand at 78.575 kb on + strand at 78.708 kb on + strand, within GFF722 at 78.755 kb on + strand, within GFF722 at 78.791 kb on + strand, within GFF722 at 78.821 kb on + strand, within GFF722 at 78.874 kb on + strand, within GFF722 at 78.874 kb on + strand, within GFF722 at 78.875 kb on - strand, within GFF722 at 78.912 kb on - strand, within GFF722 at 78.937 kb on + strand, within GFF722 at 78.937 kb on + strand, within GFF722 at 78.938 kb on - strand, within GFF722 at 78.938 kb on - strand, within GFF722 at 79.313 kb on - strand, within GFF722 at 79.362 kb on - strand, within GFF722 at 79.543 kb on + strand, within GFF722 at 79.866 kb on + strand, within GFF722 at 79.866 kb on + strand, within GFF722 at 80.038 kb on + strand, within GFF722 at 80.039 kb on - strand, within GFF722 at 80.039 kb on - strand, within GFF722 at 80.111 kb on + strand, within GFF722 at 80.112 kb on - strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.115 kb on + strand, within GFF722 at 80.116 kb on - strand, within GFF722 at 80.116 kb on - strand, within GFF722 at 80.116 kb on - strand, within GFF722 at 80.124 kb on - strand, within GFF722 at 80.435 kb on - strand, within GFF722 at 80.490 kb on - strand, within GFF722 at 80.574 kb on - strand, within GFF722 at 80.651 kb on + strand at 80.652 kb on - strand at 80.725 kb on + strand at 80.737 kb on + strand at 80.778 kb on - strand at 80.847 kb on - strand at 80.847 kb on - strand at 80.890 kb on - strand at 80.909 kb on + strand at 80.946 kb on + strand, within GFF723 at 80.960 kb on + strand, within GFF723 at 80.971 kb on + strand, within GFF723 at 81.226 kb on + strand, within GFF723 at 81.227 kb on - strand, within GFF723 at 81.228 kb on + strand, within GFF723 at 81.228 kb on + strand, within GFF723 at 81.440 kb on + strand, within GFF723 at 81.615 kb on - strand, within GFF723 at 81.692 kb on + strand at 81.693 kb on - strand at 81.727 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 77,520 - -0.0 77,520 - +0.1 77,524 - -0.3 77,584 + +0.2 77,595 - -0.2 77,632 - GFF721 0.13 -1.3 77,668 + GFF721 0.18 -0.4 77,741 - GFF721 0.27 -0.0 77,820 + GFF721 0.37 -0.0 77,860 + GFF721 0.41 -0.1 77,861 - GFF721 0.42 -0.4 77,931 - GFF721 0.50 -0.1 77,964 + GFF721 0.54 +0.1 78,023 + GFF721 0.62 -0.5 78,024 - GFF721 0.62 -0.1 78,096 + GFF721 0.71 +0.2 78,205 + GFF721 0.84 +0.8 78,364 - +0.1 78,387 + -0.1 78,419 + +0.0 78,419 + +0.0 78,420 - -0.1 78,420 - -0.0 78,440 + -0.6 78,442 + -0.3 78,575 + +0.4 78,708 + GFF722 0.12 +0.7 78,755 + GFF722 0.14 -0.1 78,791 + GFF722 0.16 -0.7 78,821 + GFF722 0.17 +0.4 78,874 + GFF722 0.19 +2.3 78,874 + GFF722 0.19 -0.1 78,875 - GFF722 0.19 +0.0 78,912 - GFF722 0.21 +0.2 78,937 + GFF722 0.22 +0.6 78,937 + GFF722 0.22 -0.2 78,938 - GFF722 0.22 -0.7 78,938 - GFF722 0.22 +0.3 79,313 - GFF722 0.37 -0.6 79,362 - GFF722 0.39 +1.3 79,543 + GFF722 0.47 -0.0 79,866 + GFF722 0.60 -0.2 79,866 + GFF722 0.60 -1.0 80,038 + GFF722 0.67 -0.2 80,039 - GFF722 0.67 -0.1 80,039 - GFF722 0.67 -0.4 80,111 + GFF722 0.70 -0.6 80,112 - GFF722 0.70 +0.5 80,115 + GFF722 0.70 -0.1 80,115 + GFF722 0.70 -1.7 80,115 + GFF722 0.70 +0.4 80,115 + GFF722 0.70 +0.3 80,116 - GFF722 0.70 -0.0 80,116 - GFF722 0.70 -0.2 80,116 - GFF722 0.70 -0.2 80,124 - GFF722 0.70 -0.1 80,435 - GFF722 0.83 +0.1 80,490 - GFF722 0.85 -0.7 80,574 - GFF722 0.89 +0.1 80,651 + +0.2 80,652 - -1.3 80,725 + +0.8 80,737 + -0.6 80,778 - +0.2 80,847 - -0.6 80,847 - +0.3 80,890 - +0.6 80,909 + -0.0 80,946 + GFF723 0.11 +0.5 80,960 + GFF723 0.12 -0.5 80,971 + GFF723 0.14 -0.1 81,226 + GFF723 0.42 -0.2 81,227 - GFF723 0.42 -0.6 81,228 + GFF723 0.42 -0.1 81,228 + GFF723 0.42 +0.4 81,440 + GFF723 0.66 +2.4 81,615 - GFF723 0.85 -0.1 81,692 + +0.3 81,693 - -0.5 81,727 - -0.0
Or see this region's nucleotide sequence