Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF68 and GFF69 are separated by 107 nucleotides GFF69 and GFF70 overlap by 1 nucleotides GFF70 and GFF71 overlap by 1 nucleotides GFF71 and GFF72 overlap by 4 nucleotides
GFF68 - Glutamate-1-semialdehyde aminotransferase (EC 5.4.3.8), at 50,598 to 51,878
GFF68
GFF69 - Uncharacterized protein YadU in stf fimbrial cluster, at 51,986 to 52,825
GFF69
GFF70 - Minor fimbrial subunit StfG, at 52,825 to 53,355
GFF70
GFF71 - Minor fimbrial subunit StfF, at 53,355 to 53,831
GFF71
GFF72 - Minor fimbrial subunit StfE, at 53,828 to 54,340
GFF72
Position (kb)
52
53
54 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 51.902 kb on - strand at 52.001 kb on - strand at 52.003 kb on + strand at 52.003 kb on + strand at 52.005 kb on + strand at 52.005 kb on + strand at 52.069 kb on + strand at 52.156 kb on + strand, within GFF69 at 52.160 kb on - strand, within GFF69 at 52.239 kb on + strand, within GFF69 at 52.239 kb on + strand, within GFF69 at 52.239 kb on + strand, within GFF69 at 52.240 kb on - strand, within GFF69 at 52.244 kb on - strand, within GFF69 at 52.247 kb on + strand, within GFF69 at 52.248 kb on - strand, within GFF69 at 52.248 kb on - strand, within GFF69 at 52.327 kb on + strand, within GFF69 at 52.449 kb on + strand, within GFF69 at 52.449 kb on + strand, within GFF69 at 52.449 kb on + strand, within GFF69 at 52.559 kb on + strand, within GFF69 at 52.560 kb on - strand, within GFF69 at 52.560 kb on - strand, within GFF69 at 52.648 kb on - strand, within GFF69 at 52.648 kb on - strand, within GFF69 at 52.650 kb on + strand, within GFF69 at 52.651 kb on - strand, within GFF69 at 52.714 kb on + strand, within GFF69 at 52.719 kb on + strand, within GFF69 at 52.720 kb on - strand, within GFF69 at 52.720 kb on - strand, within GFF69 at 52.723 kb on - strand, within GFF69 at 52.828 kb on + strand at 52.828 kb on + strand at 53.031 kb on - strand, within GFF70 at 53.034 kb on + strand, within GFF70 at 53.034 kb on + strand, within GFF70 at 53.036 kb on + strand, within GFF70 at 53.146 kb on - strand, within GFF70 at 53.490 kb on - strand, within GFF71 at 53.730 kb on - strand, within GFF71 at 53.835 kb on + strand at 54.257 kb on - strand, within GFF72
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 51,902 - +0.1 52,001 - -0.2 52,003 + +0.1 52,003 + -0.0 52,005 + +0.6 52,005 + +0.6 52,069 + +0.4 52,156 + GFF69 0.20 +0.5 52,160 - GFF69 0.21 -2.4 52,239 + GFF69 0.30 -0.3 52,239 + GFF69 0.30 +0.4 52,239 + GFF69 0.30 +0.4 52,240 - GFF69 0.30 +0.2 52,244 - GFF69 0.31 +0.1 52,247 + GFF69 0.31 -0.7 52,248 - GFF69 0.31 -0.4 52,248 - GFF69 0.31 -0.0 52,327 + GFF69 0.41 -0.0 52,449 + GFF69 0.55 +0.2 52,449 + GFF69 0.55 +0.1 52,449 + GFF69 0.55 +1.8 52,559 + GFF69 0.68 +0.3 52,560 - GFF69 0.68 -0.2 52,560 - GFF69 0.68 +0.5 52,648 - GFF69 0.79 +0.0 52,648 - GFF69 0.79 +0.3 52,650 + GFF69 0.79 +0.6 52,651 - GFF69 0.79 -0.1 52,714 + GFF69 0.87 -0.6 52,719 + GFF69 0.87 +0.2 52,720 - GFF69 0.87 -0.3 52,720 - GFF69 0.87 +0.3 52,723 - GFF69 0.88 -0.1 52,828 + -0.3 52,828 + +0.8 53,031 - GFF70 0.39 +0.5 53,034 + GFF70 0.39 -0.1 53,034 + GFF70 0.39 +0.4 53,036 + GFF70 0.40 +0.1 53,146 - GFF70 0.60 +0.4 53,490 - GFF71 0.28 +0.1 53,730 - GFF71 0.79 +1.1 53,835 + +0.2 54,257 - GFF72 0.84 -0.4
Or see this region's nucleotide sequence