Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4841 and GFF4842 are separated by 20 nucleotides GFF4842 and GFF4843 overlap by 4 nucleotides
GFF4841 - Glucarate dehydratase (EC 4.2.1.40), at 140,505 to 141,845
GFF4841
GFF4842 - Glucarate dehydratase (EC 4.2.1.40), at 141,866 to 143,206
GFF4842
GFF4843 - D-glucarate permease, at 143,203 to 144,561
GFF4843
Position (kb) 141
142
143
144 Strain fitness (log2 ratio) -1
0
1
2 at 140.877 kb on + strand, within GFF4841 at 140.945 kb on + strand, within GFF4841 at 140.978 kb on + strand, within GFF4841 at 141.094 kb on + strand, within GFF4841 at 141.094 kb on + strand, within GFF4841 at 141.099 kb on - strand, within GFF4841 at 141.525 kb on - strand, within GFF4841 at 141.544 kb on - strand, within GFF4841 at 141.710 kb on + strand, within GFF4841 at 141.710 kb on + strand, within GFF4841 at 141.716 kb on + strand at 141.719 kb on + strand at 141.776 kb on + strand at 141.777 kb on - strand at 141.777 kb on - strand at 141.777 kb on - strand at 141.831 kb on - strand at 141.839 kb on + strand at 141.840 kb on - strand at 141.845 kb on + strand at 141.857 kb on + strand at 142.118 kb on - strand, within GFF4842 at 142.118 kb on - strand, within GFF4842 at 142.157 kb on - strand, within GFF4842 at 142.343 kb on - strand, within GFF4842 at 142.445 kb on + strand, within GFF4842 at 142.445 kb on + strand, within GFF4842 at 142.445 kb on + strand, within GFF4842 at 142.446 kb on - strand, within GFF4842 at 142.446 kb on - strand, within GFF4842 at 142.516 kb on + strand, within GFF4842 at 142.752 kb on + strand, within GFF4842 at 142.753 kb on - strand, within GFF4842 at 142.826 kb on - strand, within GFF4842 at 142.992 kb on - strand, within GFF4842 at 142.992 kb on - strand, within GFF4842 at 143.026 kb on - strand, within GFF4842 at 143.136 kb on - strand at 143.140 kb on + strand at 143.141 kb on - strand at 143.239 kb on - strand at 143.244 kb on + strand at 143.268 kb on - strand at 143.304 kb on - strand at 143.345 kb on + strand, within GFF4843 at 143.346 kb on - strand, within GFF4843 at 143.394 kb on + strand, within GFF4843 at 143.446 kb on + strand, within GFF4843 at 143.496 kb on + strand, within GFF4843 at 143.528 kb on + strand, within GFF4843 at 143.529 kb on - strand, within GFF4843 at 143.772 kb on + strand, within GFF4843 at 143.772 kb on + strand, within GFF4843 at 143.772 kb on + strand, within GFF4843 at 143.778 kb on - strand, within GFF4843 at 143.805 kb on + strand, within GFF4843 at 143.805 kb on + strand, within GFF4843 at 143.806 kb on - strand, within GFF4843 at 143.806 kb on - strand, within GFF4843 at 143.855 kb on - strand, within GFF4843 at 143.883 kb on + strand, within GFF4843 at 143.912 kb on + strand, within GFF4843 at 143.946 kb on + strand, within GFF4843 at 143.947 kb on - strand, within GFF4843 at 143.977 kb on - strand, within GFF4843
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 140,877 + GFF4841 0.28 +0.1 140,945 + GFF4841 0.33 +0.0 140,978 + GFF4841 0.35 -0.4 141,094 + GFF4841 0.44 +1.8 141,094 + GFF4841 0.44 -0.1 141,099 - GFF4841 0.44 +0.5 141,525 - GFF4841 0.76 -0.3 141,544 - GFF4841 0.77 +0.8 141,710 + GFF4841 0.90 -0.2 141,710 + GFF4841 0.90 +0.2 141,716 + -0.1 141,719 + +0.2 141,776 + +0.8 141,777 - +0.5 141,777 - +0.4 141,777 - -0.3 141,831 - -0.2 141,839 + +0.1 141,840 - +1.4 141,845 + +0.0 141,857 + +0.3 142,118 - GFF4842 0.19 +0.2 142,118 - GFF4842 0.19 +0.1 142,157 - GFF4842 0.22 -0.4 142,343 - GFF4842 0.36 -0.3 142,445 + GFF4842 0.43 -0.4 142,445 + GFF4842 0.43 +0.2 142,445 + GFF4842 0.43 -0.1 142,446 - GFF4842 0.43 +0.1 142,446 - GFF4842 0.43 -0.3 142,516 + GFF4842 0.48 -0.3 142,752 + GFF4842 0.66 +0.1 142,753 - GFF4842 0.66 -0.3 142,826 - GFF4842 0.72 -0.1 142,992 - GFF4842 0.84 -0.0 142,992 - GFF4842 0.84 -0.5 143,026 - GFF4842 0.87 -0.1 143,136 - +0.5 143,140 + +1.3 143,141 - +0.0 143,239 - +0.4 143,244 + +0.2 143,268 - -0.6 143,304 - -0.2 143,345 + GFF4843 0.10 -0.4 143,346 - GFF4843 0.11 -0.6 143,394 + GFF4843 0.14 +0.2 143,446 + GFF4843 0.18 -0.6 143,496 + GFF4843 0.22 -0.3 143,528 + GFF4843 0.24 -0.2 143,529 - GFF4843 0.24 +0.4 143,772 + GFF4843 0.42 -0.2 143,772 + GFF4843 0.42 +0.1 143,772 + GFF4843 0.42 -0.2 143,778 - GFF4843 0.42 +0.4 143,805 + GFF4843 0.44 +0.2 143,805 + GFF4843 0.44 -0.3 143,806 - GFF4843 0.44 -0.4 143,806 - GFF4843 0.44 +0.4 143,855 - GFF4843 0.48 +0.3 143,883 + GFF4843 0.50 +0.4 143,912 + GFF4843 0.52 -0.2 143,946 + GFF4843 0.55 +0.0 143,947 - GFF4843 0.55 -0.0 143,977 - GFF4843 0.57 -0.2
Or see this region's nucleotide sequence