Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4779 and GFF4780 are separated by 4 nucleotides GFF4780 and GFF4781 overlap by 4 nucleotides GFF4781 and GFF4782 overlap by 7 nucleotides
GFF4779 - Hydroxypyruvate isomerase (EC 5.3.1.22), at 87,815 to 88,591
GFF4779
GFF4780 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 88,596 to 89,234
GFF4780
GFF4781 - FIG00641944: hypothetical protein, at 89,231 to 90,493
GFF4781
GFF4782 - D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 90,487 to 91,410
GFF4782
Position (kb)
89
90
91 Strain fitness (log2 ratio)
-1
0
1 at 88.436 kb on - strand, within GFF4779 at 88.446 kb on - strand, within GFF4779 at 88.541 kb on - strand at 88.629 kb on + strand at 88.695 kb on - strand, within GFF4780 at 88.752 kb on + strand, within GFF4780 at 88.820 kb on - strand, within GFF4780 at 88.820 kb on - strand, within GFF4780 at 88.944 kb on + strand, within GFF4780 at 88.945 kb on - strand, within GFF4780 at 88.945 kb on - strand, within GFF4780 at 89.003 kb on + strand, within GFF4780 at 89.511 kb on + strand, within GFF4781 at 89.512 kb on - strand, within GFF4781 at 90.111 kb on + strand, within GFF4781 at 90.112 kb on - strand, within GFF4781 at 90.112 kb on - strand, within GFF4781 at 90.247 kb on - strand, within GFF4781 at 90.255 kb on + strand, within GFF4781 at 90.601 kb on - strand, within GFF4782 at 90.601 kb on - strand, within GFF4782 at 90.605 kb on - strand, within GFF4782 at 90.708 kb on - strand, within GFF4782 at 90.708 kb on - strand, within GFF4782 at 90.782 kb on + strand, within GFF4782 at 90.782 kb on + strand, within GFF4782 at 90.782 kb on + strand, within GFF4782 at 90.782 kb on + strand, within GFF4782 at 90.783 kb on - strand, within GFF4782 at 90.846 kb on + strand, within GFF4782 at 91.440 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 88,436 - GFF4779 0.80 +1.2 88,446 - GFF4779 0.81 -0.9 88,541 - +0.3 88,629 + -0.2 88,695 - GFF4780 0.15 -0.2 88,752 + GFF4780 0.24 +0.7 88,820 - GFF4780 0.35 -0.1 88,820 - GFF4780 0.35 +0.9 88,944 + GFF4780 0.54 -0.3 88,945 - GFF4780 0.55 +0.9 88,945 - GFF4780 0.55 +0.3 89,003 + GFF4780 0.64 +0.6 89,511 + GFF4781 0.22 +0.3 89,512 - GFF4781 0.22 +0.2 90,111 + GFF4781 0.70 +1.3 90,112 - GFF4781 0.70 -0.0 90,112 - GFF4781 0.70 +0.3 90,247 - GFF4781 0.80 +0.5 90,255 + GFF4781 0.81 +0.3 90,601 - GFF4782 0.12 -0.2 90,601 - GFF4782 0.12 -0.2 90,605 - GFF4782 0.13 +0.2 90,708 - GFF4782 0.24 +0.3 90,708 - GFF4782 0.24 -0.2 90,782 + GFF4782 0.32 +0.1 90,782 + GFF4782 0.32 +0.1 90,782 + GFF4782 0.32 -0.9 90,782 + GFF4782 0.32 -0.2 90,783 - GFF4782 0.32 -0.0 90,846 + GFF4782 0.39 +0.7 91,440 - +0.1
Or see this region's nucleotide sequence